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prospective field study of transovarial dengue-virus transmission by two different forms of aedes aegypti in an urban area of bangkok, thailand.a prospective field study was conducted to determine transovarial dengue-virus transmission in two forms of aedes aegypti mosquitoes in an urban district of bangkok, thailand. immature aedes mosquitoes were collected monthly for one year and reared continuously until adulthood in the laboratory. mosquitoes assayed for dengue virus were processed in pools and their dengue virus infection status was determined by one-step rt-pcr and nested-pcr methods. of a total 15,457 newly emerged adult ae. aeg ...201121635652
control of mosquito vectors of tropical infectious diseases: (2) pyrethroid susceptibility of aedes aegypti ( l) collected from different sites in thailand.four strains (ss, bs, a and b) of aedes aegypti collected from different sites in bangkok and at different times were examined for their pyrethroid susceptibility. mosquito coils containing dl, d-t80-allethrin, d, d-t-prallethrin and methoxymethyl-tetrafluorobenzyl tetramethyl-cyclopropanecarboxylate (k-3050) with or without a synergist were tested by the 25 m3 semi-field test method. one strain (ss) was the most susceptible with kt50 values of about < 30 minutes for all mosquito coils, while th ...200818564707
trend of temephos resistance in aedes (stegomyia) mosquitoes in thailand during 2003-2005.the diagnostic dose for temephos susceptibility test was established based on aedes aegypti, the susceptible bora (french polynesia) strain, for practical and routine use. the diagnostic dose was subsequently used to evaluate the susceptibility/resistance status in f1 progenies of field-collected samples from bangkok and various parts of thailand. it appeared that ae. aegypti mosquitoes of one collection site each in bangkok, nakhon sawan (northcentral), and nakhon ratchasrima (northeast) were r ...200717540057
insecticide susceptible/resistance status in aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in thailand during 2003-2005.susceptibility baselines and diagnostic doses of the technical grade insecticides deltamethrin, permethrin, fenitrothion, and propoxur were established based on aedes aegypti (l.), bora (french polynesia), a reference susceptible strain. field-collected aedes mosquitoes from each part of thailand were subjected to bioassay for their susceptibility to the diagnostic doses of each insecticide. almost all ae. aegypti collected were incipient resistant or resistant to deltamethrin and permethrin, ex ...200717461081
genetic structure and gene flow among aedes aegypti (diptera: culicidae) populations from central thailand.isozymes from five wild-caught populations of aedes aegypti (l.) were compared using starch gel electrophoresis to estimate rates of gene flow between and among geographically close mosquito populations. ae. aegypti were collected from five different locations in bangkok, thailand. one collection was obtained from central bangkok (huai-khwang); the other four samples were obtained from surrounding areas (districts latkrabang, ratburana, laksi, and bangkok noi). based on 24 loci (17 enzyme system ...200516119549
prevalence of dengue virus in aedes mosquitoes during dry season by semi-nested reverse transcriptase-polymerase chain reaction (semi-nested rt-pcr).dengue hemorrhagic fever remains a major health concern in thailand. much effort has focused on the prevention and control of the disease. detection of dengue virus infection rate in mosquitoes would evaluate dengue control programs and predict the epidemics of dengue hemorrhagic fever. to determine dengue virus infection rate in mosquitoes by semi-nested rt-pcr. a total of 400 mosquitoes were collected from rom kao community representing a crowded community and another 9 non-crowded communities ...200416083176
characterization of deltamethrin resistance in field populations of aedes aegypti in thailand.five field collections of adult aedes aegypti mosquitoes from different areas in bangkok and pathum thani provinces were subjected to susceptibility tests against deltamethrin. low levels of resistance were detected among all populations tested (rr50 = 8-17.2) compared to the susceptible strain, bora (french polynesia). among the five populations tested, the bkh (bang khen, bangkok) and psc (phasicharoen, bangkok) populations showed a higher level of deltamethrin resistance than the other three ...200516007969
dengue and its vectors in thailand: introduction to the study and seasonal distribution of aedes larvae.a study was conducted from 1989 to 1994 that included surveillance for dengue and its mosquito vectors in 3 villages and 2 schools in chachoengsao province, 100 km east of bangkok, thailand. the study is introduced, and results of larval surveys for aedes mosquitoes (predominantly aedes aegypti (l.)), with 94, 86, and 90% of positive containers infested by this species in the hot, wet, and cool seasons, respectively), are described. these surveys were conducted in 1990-1991 during each of the 3 ...200212408663
transmission thresholds for dengue in terms of aedes aegypti pupae per person with discussion of their utility in source reduction efforts.the expense and ineffectiveness of drift-based insecticide aerosols to control dengue epidemics has led to suppression strategies based on eliminating larval breeding sites. with the notable but short-lived exceptions of cuba and singapore, these source reduction efforts have met with little documented success; failure has chiefly been attributed to inadequate participation of the communities involved. the present work attempts to estimate transmission thresholds for dengue based on an easily-de ...200010761719
lessons of aedes aegypti control in thailand.the incidence of dengue haemorrhagic fever (dhf) in thailand has increased cyclically since the first recognized outbreak in 1958. without an effective vaccine against dengue, and considering the clinical difficulty of treating dhf cases, vector control is needed to prevent dengue transmission. since the establishment of the who aedes research unit in 1964, continued since 1973 as the who collaborating centre at the department of medical research in bangkok, much operational research has been ca ...19938435481
dynamic life table model for aedes aegypti (diptera: culicidae): simulation results and validation.the container-inhabiting mosquito simulation model (cimsim) is a weather-driven, dynamic life table simulation model of aedes aegypti (l.) and similar nondiapausing aedes mosquitoes that inhabit artificial and natural containers. this paper presents a validation of cimsim simulating ae. aegypti using several independent series of data that were not used in model development. validation data sets include laboratory work designed to elucidate the role of diet on fecundity and rates of larval devel ...19938271243
studies on mosquitoes and their natural enemies in bangkok. 2. insecticide susceptibility of the larvae of culex pipiens fatigans, aedes aegypti and the mosquito-eating fish lebistes reticulatus. 196514326223
evaluation of mosquito densoviruses for controlling aedes aegypti (diptera: culicidae): variation in efficiency due to virus strain and geographic origin of mosquitoes.four mosquito densovirus strains were assayed for mortality and infectivity against aedes aegypti larvae from different geographic regions. the viral titers were quantified by real-time pcr using taqman technology. firstinstar larvae were exposed to the same titer of each densovirus strain for 48 hours. all strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. aaldnv-exposed larvae had the highest mortality rate. t ...200818458314
a study of biting habits of aedes aegypti in bangkok, thailand. 19705312528
the effectiveness of ultra-low-volume applications of malathion at a rate of 3 us fluid ounces per acre in controlling aedes aegypti in thailand.ultra-low-volume (ulv) aerial applications of malathion at a rate of 3 us fl oz per acre (219 ml/ha) for the control of aedes aegypti and culex pipiens fatigans were evaluated in 2 areas near bangkok, thailand. a housing project of the royal thai air force received 2 applications a week apart while the village of bang khen received 1 application. bioassays, oil-sensitive red dye cards, landing-rate counts, ovitraps, and light-traps were used to evaluate the effects of the treatment. a comparison ...19705309518
the magnitude of seasonal changes in larval populations of aedes aegypti in bangkok, thailand.weekly surveys of aedes aegypti larvae were made during 1966-67 in three localities in bangkok in order to detect and measure any changes in the population which might be correlated with the seasons. the larval habitats were divided into water-jars and miscellaneous containers. only the data from alternate weekly surveys were analysed. analysis of variance showed that the changes in the numbers of habitats occupied varied greatly both by month and by locality. it is concluded that there was some ...19705312256
pilot studies of abate as a larvicide for control of aedes aegypti in bangkok, thailand. 19725028856
development and reversion of ddt resistance in an aedes aegypti population in bangkok, thailand. 19715316918
a field trial of abate larvicide for the control of aedes aegypti in bangkok, thailand. 19724537857
effect of temperature on the vector efficiency of aedes aegypti for dengue 2 virus.the effect of temperature on the ability of aedes aegypti to transmit dengue (den) 2 virus to rhesus monkeys was assessed as a possible explanation for the seasonal variation in the incidence of dengue hemorrhagic fever in bangkok, thailand. in two laboratory experiments, a bangkok strain of ae. aegypti was allowed to feed upon viremic monkeys infected with den-2 virus. blood-engorged mosquitoes were separated into two groups and retained at constant temperatures. virus infection and transmissio ...19873812879
residual effectiveness of ulv aerosols against aedes aegypti in bangkok: a study of sumithion and malathion applied by a portable ulv machine. 19734749075
field studies on the gonotrophic cycle of aedes aegypti in bangkok, thailand. 19734707760
studies on the life budget of aedes aegypti in wat samphaya, bangkok, thailand.for a complete understanding of the epidemiology of a vector-borne disease, a knowledge of the bionomics of the vector is needed. the development of aedes aegypti was studied in wat samphaya, bangkok, thailand, where work on the adult biology had been carried out the previous year (1966-67). particular attention was given to the variation in the numbers of immature stages of the mosquito in relation to the known seasonal incidence of dengue haemorrhagic fever. of the three types of water contain ...19724537483
bionomics of toxorhynchites splendens in the larval habitats of aedes aegypti in bangkok, thailand. 19705313363
effectiveness of different insecticides and formulations against aedes aegypti larvae in ant traps in bangkok, thailand. 19695308706
replicate surveys of larval habitats of aedes aegypti in relation to dengue haemorrhagic fever in bangkok, thailand.dengue haemorrhagic fever in bangkok and thonburi occurs principally during the wet season. the mosquito vector is aedes aegypti. a study was made of the larval habitats of a. aegypti in 14 localities, at three different times of the year, to determine whether there were fluctuations in the a. aegypti population, as measured by the number of occupied habitats, which could be correlated with the incidence of the infection.the habitats were classified into 6 categories and a single larva was colle ...19695307596
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