Publications

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[factors associated the seropositivity for babesia, toxoplasma, neospora e leishmania in dogs attended at nine veterinary clinics in the municipality of lavras, mg].the aim of the present study was to determine the frequency and evaluate the infuence of age, sex and breed in seropositivity anti-babesia canis, toxoplasma gondii, leishmania (l.) chagasi and neospora caninum, by means of the indirect immunofuorescence antibody test (ifat), in serum samples collected from dogs attended in nine private veterinary clinics in municipality of lavras, minas gerais, brazil, from august 2000 to april 2002. of 300 dogs, 73.3% were seropositive (ifat>or=1:80) to b. cani ...200920040191
evaluation of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, by different serological methods.toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. considering the scarce information on the occurrence of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, this study aimed to investigate the frequency of antibodies against these parasites in sheep sera from this region by using different serological methods. a total of 155 sheep serum samples were analyze ...201021075529
pathogens of wild maned wolves (chrysocyon brachyurus) in brazil.the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ...201223060508
seroprevalence and risk factors of caprine toxoplasmosis in minas gerais, brazil.the objective of this work was to carry out a study on caprine toxoplasmosis in the state of minas gerais, brazil. to determine the prevalence of toxoplasmosis in goats in minas gerais, 767 sera from goats were tested by elisa (enzyme-linked immunosorbent assay) and ifat (indirect fluorescence antibody test). the prevalence of antibodies to toxoplasma gondii was 43.0% and 46.0% by elisa and ifat, respectively. it was observed that 26.8% of the goats show low-avidity igg to t. gondii. these resul ...200919091475
characterization of toxoplasma gondii from domestic animals from minas gerais, brazil.in an attempt to isolate and characterize toxoplasma gondii from the state of minas gerais, brazil, musculature samples from 72 pigs, 25 dogs, 28 free-range chickens and 50 chickens produced in industrialized farms were collected. antibodies to t. gondii have not been detected in pigs, but were found in nine (40.9 %) out of 22 dogs, and in 15 (53.6 %) of 28 free range chickens. t. gondii was not isolated from pigs and industrialized chickens, but from eight dogs and 11 free range chickens. in or ...200616800123
a multihousehold study reveals a positive correlation between age, severity of ocular toxoplasmosis, and levels of glycoinositolphospholipid-specific immunoglobulin a.a cross-sectional household study involving 499 individuals was undertaken in an area of minas gerais state, brazil, where infection with toxoplasma gondii is endemic. nearly 50% (n=247) of the sample had t. gondii-specific antibodies, even individuals in the 5-9-year-old age group. approximately 12.5% (n=28) of a random subsample of participants who were positive for t. gondii antibodies had ocular lesions associated with t. gondii infection. the frequency of ocular toxoplasmosis increased sign ...200415195258
a comparative study of congenital toxoplasmosis between public and private hospitals from uberlândia, mg, brazil.the main purpose of the present study was to examine if there is difference in terms of incidence rates of congenital toxoplasmosis among populations assisted in public and private hospitals from uberlândia, state of minas gerais, brazil. a total of 805 serum samples from cord blood were collected, being 500 from public hospital and 305 from private hospital, and all patients answered a questionnaire about pregnancy and newborns. an indirect enzyme linked immunosorbent assay (elisa) was performe ...200415057340
seroprevalence of toxoplasma gondii infection in goats by the indirect haemagglutination, immunofluorescence and immunoenzymatic tests in the region of uberlândia, brazil.a comparative study of the indirect haemagglutination (iha), immunofluorescence (ifat) and immunoenzymatic (elisa) tests was carried out to determine the prevalence of toxoplasma gondii antibodies in goats. one hundred seventy-four serum samples were obtained from four goat herds from the region of uberlândia, state of minas gerais. the distribution of the animals, according to their origin, was as follow: 71 from herd i; 39 from herd ii; 37 from herd iii; and 27 from herd iv. serum samples were ...200111500772
toxoplasmosis and mental retardation--report of a case-control study.a case-control study evaluating the association between mental retardation and toxoplasmosis was conducted among 845 school children in belo horizonte, mg, brazil. cases (450) were mentally retarded children attending a public school for special education. controls (395) were children from the regular public school system. clinical and anthropometric examinations and interviews were carried out to determine risk factors for toxoplasmosis and mental retardation. diagnosis of toxoplasma gondii inf ...19938107587
[epidemiology of toxoplasma gondii infection in the municipality of ribeirâo das neves, mg. i. importance of domestic animals as sources of t. gondii infection in humans].during a six month period (june 1983 to january 1984), a case-control study was carried out to determine if the variables associated with domestic animals contact would be correlated with the prevalence of toxoplasma gondii infection. a randomized sample of 500 subjects from the population of ribeirão das neves, minas gerais, brazil was studied. a statistically significant correlation was found with cat, chicken and pig contact but none with consumption of meat, milk and eggs.19957480915
toxoplasma gondii and coxiella burneti antibodies among brazilian slaughterhouse employees.previous studies have indicated toxoplasmosis and q fever are potential hazards to persons who handle raw meat or who work in slaughterhouses. the prevalence of antibodies to t. gondii and c. burneti was studied among 144 employees of an abattoir in belo horizonte, brazil, to determine what environmental factors may be associated with the occurrence of these agents among persons who handle meat but who have not reported having toxoplasmosis or q fever. seventy-two per cent of the 144 employees w ...19751200023
seroprevalence and risk factors of toxoplasmosis in cattle from extensive and semi-intensive rearing systems at zona da mata, minas gerais state, southern brazil.concerning the infection of humans by t. gondii, limited efforts have been directed to the elucidation of the role of horizontal transmission between hosts. one of the main routes of transmission from animals to humans occurs through the ingestion of raw or insufficiently cooked meat. however, even though the detection of t. gondii in meat constitutes an important short-term measure, control strategies can only be accomplished by a deeper understanding of the epidemiology of toxoplasmosis. the p ...201323800302
adverse socioeconomic conditions and oocyst-related factors are associated with congenital toxoplasmosis in a population-based study in minas gerais, brazil.congenital toxoplasmosis is a public health problem in brazil. this study aimed to determine risk factors associated with congenital toxoplasmosis in minas gerais which is the second largest brazilian state based on number of inhabitants, and its territorial extension is larger than that of france.201424523920
overlapping toxoplasma gondii genotypes circulating in domestic animals and humans in southeastern brazil.although several toxoplasma gondii genotyping studies have been performed in brazil, studies of isolates from animals in the state of minas gerais are rare. the objective of this study was to conduct a genotypic characterization of t. gondii isolates obtained from dogs, free-range chickens, and humans in minas gerais and to verify whether the t. gondii genotypes circulating in domestic animals correspond to the genotypes detected in humans. genetic variability was assessed by restricted fragment ...201424587295
experimental infection of calomys callosus with atypical strains of toxoplasma gondii shows gender differences in severity of infection.there is a significant genetic diversity of toxoplasma gondii in brazil. two parasite isolates were recently obtained from chickens in uberlândia, minas gerais state, brazil, namely, tgchbrud1 and tgchbrud2. in this study, we investigated calomys callosus susceptibility to these atypical t. gondii strains. male and female animals were intraperitoneally infected with tachyzoites and monitored to evaluate body weight change, morbidity, and mortality. immunohistochemical assay and qpcr were perform ...201424781027
phenotypic and genotypic characterization of two toxoplasma gondii isolates in free-range chickens from uberlândia, brazil.the aim of this study was to determine the seroprevalence of toxoplasma gondii infection in free-range chickens from uberlândia, minas gerais state, brazil, and characterize the genotypic and phenotypic features of two isolates of this parasite, considering the importance of these hosts in the epidemiology of toxoplasmosis. serum samples from 108 free-range chickens were obtained from ten different districts, and submitted to the modified agglutination test (mat) for the presence of anti-t. gond ...201626743347
seroepidemiology of sarcocystis neurona, toxoplasma gondii and neospora spp. among horses in the south of the state of minas gerais, brazil.the present study used the indirect fluorescent antibody test (ifat) to determine the seroprevalence of sarcocystis neurona, toxoplasma gondii and neospora spp., and evaluated the variables associated with these infections among 506 apparently healthy horses, reared in the south of the state of minas gerais, brazil. this study was conducted between april 2012 and october 2013. among the horses, the true prevalence of s. neurona was 26% (95% ci: 22.0-30.4%), t. gondii 19.9% (95% ci: 15.5-24.8%) a ...201627334814
genetic characterization of toxoplasma gondii revealed highly diverse genotypes for isolates from newborns with congenital toxoplasmosis in southeastern brazil.recent studies of toxoplasma gondii isolates from animals in brazil have revealed high genetic diversity. many of these isolates are virulent to mice. it is speculated that these isolates may also be virulent to humans. however, there is very limited data regarding t. gondii strains from human infection. therefore, it is not clear whether there is any association between parasite genotypes and disease phenotypes. in this study, a total of 27 t. gondii strains were isolated from humans with conge ...201323284022
association between igg subclasses against toxoplasma gondii and clinical signs in newborns with congenital toxoplasmosis.the aim of this study was to evaluate the association between clinical signs of congenital toxoplasmosis and igg subclasses found in newborns participating in the minas gerais state neonatal screening program.201322935868
genetic polymorphisms and phenotypic profiles of sulfadiazine-resistant and sensitive toxoplasma gondii isolates obtained from newborns with congenital toxoplasmosis in minas gerais, brazil.previous toxoplasma gondii studies revealed that mutations in the dhps (dihydropteroate synthase) gene are associated with resistance to sulfonamides. although brazilian strains are genotypically different, very limited data are available regarding the susceptibility of strains obtained from human to sulfonamides. the aim of this study was to evaluate the efficacy of sulfadiazine (sdz) against brazilian isolates of t. gondii and verify whether isolates present polymorphisms in the dhps gene. we ...201728118394
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