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herd-level prevalence and associated risk factors for toxoplasma gondii, neospora caninum, chlamydia abortus and bovine viral diarrhoea virus in commercial dairy and beef cattle in eastern, northern and northeastern china.although the seroprevalence of toxoplasma gondii, neospora caninum, chlamydia abortus and bovine viral diarrhea virus infection in cattle have been reported in some areas in china, most of them were conducted with small number of cattle samples and very limited districts and neglected the assessment of herd management factors associated with herd-level prevalence of these pathogen infections. thus, from september 2013 to december 2014, a large-scale seroprevalence study was conducted to determin ...201526231838
detection of neospora caninum-dna in brain tissues from pigeons in changchun, jilin (china).neospora caninum is an intracellular protozoan infecting many domestic and wild animals. the domestic chicken (gallus domesticus) and the sparrow (passer domesticus) are known as natural intermediate hosts of n. caninum, whereas the role of other birds such as pigeons is still unclear. in the present study, pigeon brain tissues collected in jilin of china were screened by n. caninum specific-nested pcr to determine whether pigeons functioned as the natural intermediate hosts of n. caninum. the p ...201526385650
low prevalence of neospora caninum and toxoplasma gondii antibodies in dogs in jilin, henan and anhui provinces of the people's republic of china.neospora caninum and toxoplasma gondii are important pathogens of worldwide distribution. n. caninum is a major cause of abortion in cattle and dogs are main reservoirs because they excrete the environmentally resistant oocysts. toxoplasmosis is a worldwide zoonosis and dogs are considered as sentinels for this parasite because of their close contact with people and cats; additionally dog meat is also used for human consumption in china. the aim of the present study was to assess the prevalence ...201425495616
seroepidemiology of human toxoplasma gondii infection in china.toxoplasmosis is an important zoonotic parasitic disease worldwide. in immune competent individuals, toxoplasma gondii preferentially infects tissues of central nervous systems, which might be an adding factor of certain psychiatric disorders. congenital transmission of t. gondii during pregnancy has been regarded as a risk factor for the health of newborn infants. while in immune-compromised individuals, the parasite can cause life-threatening infections. this study aims to investigate the prev ...201020055991
prevalence and genetic characterization of toxoplasma gondii in donkeys in northeastern china.toxolasma gondii is one of the most important obligate intracellular protozoan parasites. recently, toxoplasmosis is of increasing concerns because t. gondii not only has a worldwide distribution but also can infect virtually all warm-blooded hosts including donkeys. however, limited information is available concerning the genetic characterization of t. gondii in donkeys in northeastern china. in this study, a total of 302 brain tissue samples collected from donkeys from jilin (n=108) and liaoni ...201728807857
high prevalence of toxoplasma gondii infection in microtus fortis by seminested pcr from jilin province, northeastern china.toxoplasma gondii is an important protozoan parasite that can infect virtually all warm-blooded animals, including humans. oocysts in the environment are an important infection source. microtus fortis can be used as a sentinel reflecting the distribution of t. gondii oocysts in the environment indirectly. in the present study, we developed a seminested polymerase chain reaction (pcr) targeting t. gondii 529-base-pair repeat sequence, with high sensitivity and specificity. two hundred seventy-two ...201323050695
first report of genotyping of toxoplasma gondii in free-living microtus fortis in northeastern china.toxoplasma gondii is an intracellular protozoan parasite which imperils the health of almost all warm-blooded animals, including humans. the objective of this study was to determine genetic characterization of t. gondii in free-living microtus fortis (reed vole) in jilin province, northeastern china. a total of 104 dna samples, 74 from gongzhuling and 30 from baicheng, were extracted from lung tissues of m. fortis , and 56 (53.8%) of them were positive for t. gondii by semi-nested polymerase cha ...201424684624
prevalence and genotype of toxoplasma gondii infection in cattle from jilin province, northeastern china.abstract the prevalence and genotype of toxoplasma gondii infection in cattle in jilin province, northeastern china was investigated. a total of 1040 serum samples were collected from eight administrative regions from september to october, 2011, and antibodies to t. gondii were examined by enzyme-linked immunosorbent assay (elisa). the results showed that the overall seroprevalence of t. gondii in cattle was 12.8% (95% confidence interval [ci], 10.8-14.8%), with a higher prevalence of 22.2% (95% ...201424746235
molecular detection and genotypic characterization of toxoplasma gondii in wild waterfowls in jilin province, northeastern china.toxoplasma gondii can infect almost warm-blooded animals, including humans. limited information about t. gondii infection in wild waterfowls is available in china. the present study was conducted to determine prevalence and genotype t. gondii infection in 11 wild waterfowl species in jilin province, northeastern china. a total of 249 wild waterfowls were sampled between april and july 2013 from jilin province, and the tissue samples were collected for the detection of t. gondii by a semi-nested ...201526292258
seroprevalence and risk factors of toxoplasma gondii infection in stray dogs in northern china.toxoplasma gondii is a zoonotic protozoan of almost all species of mammals and birds. however, no information is available about seroprevalence of the pathogen in stray dogs in seven cities from inner mongolia, jilin, liaoning province, china. using the indirect hemagglutination antibody (iha) test with a cutoff titer of 1:64, 129 (14.05 %) out of 918 stray dogs were surveyed to be t. gondii seropositive. analysis of the risk factor showed that there was no statistical difference of seroprevalen ...201526403980
first report of toxoplasma gondii seroprevalence in farmed arctic foxes (vulpes lagopus) in eastern and northeastern china.until now, no information on toxoplasma gondii seroprevalence in arctic foxes (vulpes lagopus) was available in china. a serological survey was undertaken to assess t. gondii seroprevalence in farmed arctic foxes in eastern and northeastern china. antibodies to t. gondii were examined in 1346 farmed arctic foxes using the modified agglutination test (mat). a total of 113 (8.39%, 95% confidence interval [ci] 6.91-9.87) serum samples were positive to t. gondii at a 1:25 cutoff. seroprevalence of t ...201526684525
toxoplasma gondii infection in pregnant women in china.infection with toxoplasma gondii is common and usually asymptomatic, but it can have serious consequences in pregnant women if passed to the developing fetus. the aims of this study were to determine the prevalence of toxoplasmosis in pregnant women and to identify the possible risk factors associated with t. gondii infection in china. of a sample of 235 pregnant women in changchun, china, 25 (10.6%) were found by elisa to be positive for igg and none (0%) for igm. major risk factors were found ...200918822439
genetic characterization of toxoplasma gondii isolates from pigs in jilin province, northeastern china.toxoplasma gondii is prevalent in humans and animals worldwide. the present study aimed to determine the genetic diversity of t. gondii isolates from pigs in jilin province, northeastern china. a total of 100 dna samples were extracted from the hilar lymph nodes of slaughtered pigs, and 9 (9.0%, 95% confidence interval: 3.4-14.6%) were detected positive for t. gondii b1 gene by a nested polymerase chain reaction (pcr). the positive dna samples were typed at 11 genetic markers, including 10 nucle ...201626682614
first genetic characterization of toxoplasma gondii infection in arctic foxes (vulpes lagopus) in china.toxoplasma gondii can infect virtually all warm-blooded animals including foxes. however, little is known of the molecular epidemiology and genotypes of t. gondii infecting foxes in china. therefore, the present study characterized t. gondii genotypes in foxes in china for the first time. during november 2014 to october 2015, brain tissue samples collected from 264 arctic foxes (vulpes lagopus) in jilin, heilongjiang and shandong provinces were used to detect the t. gondii b1 gene by a semi-nest ...201627343765
seroprevalence and risk factors of toxoplasma gondii infection in farmed raccoon dogs (nyctereutes procyonoides) in china.toxoplasma gondii, an obligate intracellular apicomplexan parasite, can infect homoiothermal vertebrate animals, including raccoon dogs (nyctereutes procyonoides). regretfully, data on t. gondii infection in raccoon dogs were limited in china. therefore, to investigate the seroprevalence and to evaluate risk factors for t. gondii infection in raccoon dogs, a total of 1181 raccoon dog blood samples were collected from jilin and shandong provinces, china, from september to december 2014. the antib ...201727893338
molecular detection and genotypic characterization of toxoplasma gondii infection in bats in four provinces of china.toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. limited information about t. gondii infection in bats is available in china. the objective of the present study was to determine prevalence and genetic characterization of t. gondii infection in bats in jilin, liaoning, jiangxi and guangdong provinces, china.201425465220
seroprevalence of toxoplasma gondii antibodies and associated risk factors among children in shandong and jilin provinces, china.infection by the protozoan parasite toxoplasma gondii is generally prevalent in animals and humans worldwide. however, little is known about t. gondii infection among children in china. thus, the present study was conducted to detect the seroprevalence of t. gondii infection and estimate associated risk factors among children in eastern china.201525461653
seroprevalence of toxoplasma gondii infection in pigs in jilin province, northeastern china.in the present study, a total of 1,235 porcine serum samples were collected from 9 counties in jilin province (40°52'~46°18'n,121°38'~131°19'e), northeastern china from august to october 2013, and the seroprevalence of toxoplasma gondii infection was tested by indirect haemagglutination assay (iha). the results showed that antibodies to t. gondii were found in 19.1% (95% confidence interval [ci], 16.9% to 21.3%), with higher seroprevalence in the breeding boars (28.6%, 95% ci, 20.0% to 37.2%), a ...201525801260
seroprevalence and risk factors of toxoplasma gondii infection in domestic sika deer (cervus nippon) in northeastern china.toxoplasmosis is a worldwide zoonosis caused by toxoplasma gondii, which can infect warm-blooded animals and humans. a serological survey was undertaken to examine the seroprevalence and risk factors associated with t. gondii infection in sika deer in northeastern china. 114 (13.46%, 95% ci 11.16-15.76) out of 847 serum samples were positive to t. gondii by modiļ¬ed agglutination test (mat) at a 1:25 cut-off, with titers of 1:25 in 44, 1:50 in 32, 1:100 in 17, 1:500 in 11, 1:1500 or higher in 10. ...201425201489
first report of toxoplasma gondii infection in common quails (coturnix coturnix) intended for human consumption in three provinces of northeastern china.common quails are nutritious food and are also used as medicine in some parts of the world. until now, no information on toxoplasma gondii infection in common quails intended for human consumption in china is available. therefore, this study was carried out to determine the prevalence of t. gondii infection in common quails in six cities from shandong, liaoning, and jilin provinces, china. out of 620 common quails examined, 59 (9.52%) were seropositive for t. gondii by the modified agglutination ...201728437182
toxoplasma gondii infection in farmed wild boars (sus scrofa) in three cities of northeast china.the apicomplexan protozoan parasite toxoplasma gondii is a widely distributed etiological agent of foodborne illness. this parasite can cause production losses in livestock and serious disease in humans through consumption of contaminated meat. pig meat is the most likely source of human infection, and wild boars may play a role in the transmission of t. gondii by serving as a reservoir host. this study aimed to investigate the seroprevalence of antibodies to t. gondii among farmed wild boars in ...201728387529
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