Publications

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detection of viable mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and pcr.cattle with johne's disease can shed live mycobacterium avium subsp. paratuberculosis (map) in their milk, and map can survive under simulated commercial pasteurization conditions. in several studies conducted in the united kingdom and canada, map dna has been detected in retail pasteurized milk samples; however, in one study in the united kingdom viable map was identified in commercially pasteurized milk. a double-blind study involving two laboratories was undertaken to evaluate retail pasteuri ...200515895728
selenium status and antibodies to selected pathogens in white-tailed deer (odocoileus virginianus) in southern minnesota.to determine exposure to a variety of infectious diseases potentially important for native ungulates, livestock, and humans, serum samples from 114 (94 adults, 20 fawns) female white-tailed deer (odocoileus virginianus) were collected during january 2000-03 from multiple locations in southeast (se) and southwest (sw) minnesota. antibody prevalence was determined for the following pathogens: mycobacterium avium subsp. paratuberculosis, leptospira interrogans (six serovars), anaplasma marginale, b ...200818263838
decrease of johne's disease prevalence and incidence in six minnesota, usa, dairy cattle herds on a long-term management program.the objective of this prospective longitudinal field study was to describe changes in prevalence of seroconversion and fecal shedding and changes in incidence rate of seroconversion, fecal shedding and culling of milk cows for clinical signs of johne's disease (jd) in six minnesota (usa) herds participating to the jd demonstration herd project (jddhp) from 2000 to december 2005. changes in prevalence and incidence rate were evaluated in light of the owner's compliance to the jddhp using a risk a ...200918848365
the associations between culling due to clinical johne's disease or the detection of mycobacterium avium subsp. paratuberculosis fecal shedding and the diagnosis of clinical or subclinical diseases in two dairy herds in minnesota, usa.our objectives were to identify associations between clinical or subclinical diseases and subsequent culling because of clinical johne's disease (jd) or the detection of fecal shedding of mycobacterium paratuberculosis (map) after 305 days in milk (dim). a total of 1297 cows from two minnesota dairies were enrolled in the study. from study cows, fecal samples were obtained prior to calving (close-up period) and after at least 305 dim or at the time of leaving the herd (sold/dead). between 3 and ...200717368838
evaluation of the voluntary johne's disease herd status program as a source of replacement cattle.the objectives of this study were to evaluate the perceived value that enrolled producers gained from participation in the voluntary johne's disease herd status program (vjdhsp), and to evaluate the risk of infection with mycobacterium avium ssp. paratuberculosis (map) of cattle reared in a presumed johne's free environment vs. cattle raised in an environment of unknown johne's status. producers enrolled in level 3 or 4 of the vjdhsp (98 and 99% probability, respectively, of being free from map ...200616899680
detection of mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in wisconsin and minnesota by pcr.research has been focused on the detection of mycobacterium avium subspecies paratuberculosis (map) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. therefore, map contamination in milk-derived products must be investigated. we undertook a six-month study to investigate the presence of viable map and map genetic components in cheese curds purchased from retail outlets in the northern and southern regions of wisconsin and minnesota. a total of 98 r ...200616545540
characterization of minnesota dairy herds participating in a johne's disease control program and evaluation of the program risk assessment tool.the objectives of this study were to: (1) characterize minnesota dairy herds participating in a johne's disease control program (jdcp) based on herd size, milk production, and clinical johne's disease (jd) history, (2) evaluate if change in farm management practices, expressed in risk assessment (ra) total score, is associated with the change between the first and most recent elisa test herd seroprevalence or change in clinical jd culling rate, and (3) identify farm factors associated with elisa ...200616504319
the distribution of mycobacterium avium ssp. paratuberculosis in the environment surrounding minnesota dairy farms.the objective of this study was to characterize the distribution of mycobacterium paratuberculosis (map) in the environment of infected and uninfected minnesota dairy farms. eighty herds known to be infected from minnesota's johne's disease control program (jdcp) and 28 herds known to be uninfected from minnesota voluntary johne's disease herd status program (vjdhsp) were sampled. fecal samples from up to 100 cows in each herd were cultured in pools of 5 cows. two environmental samples were obta ...200415375057
evaluation of progress made by dairy and beef herds enrolled in the minnesota johne's disease control program.to evaluate progress made by cattle herds in the control of paratuberculosis (johne's disease) through participation in the minnesota johne's disease control program (mnjdcp).200819072610
clinical trial on type of calving pen and the risk of disease in holstein calves during the first 90 d of life.the objective of this study was to evaluate the efficacy of single cow calving pens that are cleaned between calvings vs. multiple cow calving pens for the prevention of calf diarrhea (scours), respiratory disease (pneumonia) and morbidity attributable to any cause. every other pregnant cow or heifer was moved to either the single cow calving pen (treatment) or the multiple cow calving pen (control) within 48-72 h prior to actual calving. the calves born in the single cow calving pens were assig ...200919193464
Evaluation of the risk of paratuberculosis in adult cows fed Mycobacterium avium subsp paratuberculosis DNA-positive or -negative colostrum as calves.To estimate the risk of subclinical Mycobacterium avium subsp paratuberculosis (MAP) infection in cows that ingested MAP DNA-positive raw colostrum as calves, compared with risk in cows that ingested MAP DNA-negative raw colostrum as calves.201122023123
mycobacterium avium subsp. paratuberculosis from free-ranging deer and rabbits surrounding minnesota dairy herds.the objectives of this study were to estimate the prevalence of mycobacterium avium subsp. paratuberculosis (map) among deer and rabbits surrounding infected and noninfected minnesota dairy farms using fecal culture, and to describe the frequency that farm management practices were used that could potentially lead to transmission of infection between these species. fecal samples from cows and the cow environment were collected from 108 minnesota dairy herds, and fecal pellets from free-ranging w ...200515745220
sensitivity of test strategies used in the voluntary johne's disease herd status program for detection of mycobacterium paratuberculosis infection in dairy cattle herds.to evaluate sensitivities at the herd level of test strategies used in the voluntary johne's disease herd status program (vjdhsp) and alternative test strategies for detecting dairy cattle herds infected with mycobacterium paratuberculosis.200212420786
amplified fragment length polymorphism to detect clonal diversity and distribution of mycobacterium avium subspecies paratuberculosis in selected minnesota dairy cattle.the molecular ecology of mycobacterium avium subspecies paratuberculosis (map), the causative agent of johne's disease, is not well understood in the united states. in this study, a dna fingerprinting method, amplified fragment length polymorphism (aflp), was used to subtype the pathogen and assess the clonal diversity of map in minnesota dairy herds. fifty-six fecal culture test-positive isolates from various minnesota counties and culture dates were analyzed in this study. the aflp identified ...200516130987
Estimated within-herd prevalence (WHP) of Mycobacterium avium subsp. paratuberculosis in a sample of Minnesota dairy herds using bacterial culture of pooled fecal samples.The objective of this study was to describe the estimated within-herd prevalence (WHP) of Mycobacterium avium subsp. paratuberculosis (Map) in a sample of infected dairy herds in Minnesota (N = 66) using test results from bacterial culture of pooled fecal samples. Fecal samples were collected from up to 100 cows in each herd and were tested using bacterial culture in pools of 5 cows based on age order. The mean herd size was 222 (44 to 1500) milking cows; the cows were predominantly Holstein. Us ...201121731181
loss of income from cows shedding mycobacterium avium subspecies paratuberculosis prior to calving compared with cows not shedding the organism on two minnesota dairy farms.quantification of the financial effect of mycobacterium paratuberculosis infection on lactation performance is essential to encourage participation of dairy cattle producers in johne's disease (jd) control programs. the objective of this study was to evaluate the differences in net income per lactation of cows shedding mycobacterium paratuberculosis before calving compared with test-negative cows. two minnesota dairies were enrolled in the study and fecal samples were collected from 1,048 cows d ...200919762809
efficacy of feeding plasma-derived commercial colostrum replacer for the prevention of transmission of mycobacterium avium subsp paratuberculosis in holstein calves.to estimate the relative risk of paratuberculosis (johne's disease [jd]) in calves fed a plasma-derived colostrum-replacement (cr) product versus raw bovine maternal colostrum (mc).200919405889
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