assessing the impact of tailored biosecurity advice on farmer behaviour and pathogen presence in beef herds in england and wales.the term 'biosecurity' encompasses many measures farmers can take to reduce the risk of pathogen incursion or spread. as the best strategy will vary between settings, veterinarians play an important role in assessing risk and providing advice, but effectiveness requires farmer acceptance and implementation. the aim of this study was to assess the effectiveness of specifically-tailored biosecurity advice packages in reducing endemic pathogen presence on uk beef suckler farms. one hundred and sixt ...201627931934
incidence of mycobacterium paratuberculosis in raw sheep and goats' milk in england, wales and northern ireland.a blind survey of 104 raw sheep and goats' milk samples (90 goat, 14 sheeps) from bulk tanks on farms throughout england, wales and northern ireland was carried out over a 5-month period (january-may 1998) in order to determine the incidence of mycobacterium paratuberculosis. each milk sample (100 ml) was divided into two 50ml portions. one portion was decontaminated with 0.75% hexadecylpyridinium chloride for 5h before culture on slopes of herrold's egg yolk medium and in bactec radiometric med ...200111230934
mycobacterium avium subsp. paratuberculosis in the catchment area and water of the river taff in south wales, united kingdom, and its potential relationship to clustering of crohn's disease cases in the city of south wales, united kingdom, a populated coastal region lies beneath hill pastures grazed by livestock in which mycobacterium avium subsp. paratuberculosis is endemic. the taff is a spate river running off the hills and through the principal city of cardiff. we sampled taff water above cardiff twice weekly from november 2001 to november 2002. m. avium subsp. paratuberculosis was detected by is900 pcr and culture. thirty-one of 96 daily samples (32.3%) were is900 pcr positive, and 12 grew m. a ...200515812047
mycobacterium avium subsp. paratuberculosis in lake catchments, in river water abstracted for domestic use, and in effluent from domestic sewage treatment works: diverse opportunities for environmental cycling and human exposure.mycobacterium avium subsp. paratuberculosis from infected animals enters surface waters and rivers in runoff from contaminated pastures. we studied the river tywi in south wales, united kingdom, whose catchment comprises 1,100 km2 containing more than a million dairy and beef cattle and more than 1.3 million sheep. the river tywi is abstracted for the domestic water supply. between august 2002 and april 2003, 48 of 70 (68.8%) twice-weekly river water samples tested positive by is900 pcr. in rive ...200616751517
is900 pcr to detect mycobacterium paratuberculosis in retail supplies of whole pasteurized cows' milk in england and wales.is900 pcr for mycobacterium paratuberculosis was applied to cream, whey, and pellet fractions of centrifuged whole cows' milk. the test and simultaneous control reactions gave correct results for spiked milk and for native milk samples obtained directly from m. paratuberculosis-free, subclinically infected, and clinically infected cows. the test was then applied to units of whole pasteurized cows' milk widely obtained from retail outlets throughout central and southern england from september 199 ...19968795236
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