Publications

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presence, characterization, and genotype profiles of mycobacterium avium subspecies paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized milk, and milk products in india by culture, pcr, and pcr-rea methods.mycobacterium avium subspecies paratuberculosis (map) causes johne's disease in ruminants, a chronic enteritis evocative of human inflammatory bowel disease. in industrialized countries map has been cultured from pasteurized milk, compounding the increasing concern that map may be zoonotic. the purpose of this study was to evaluate commercially available unpasteurized and pasteurized milk and its products for the presence of viable map or map dna from an area of northern india with a population ...201019576834
wildlife (boselaphus tragocamelus)-small ruminant (goat and sheep) interface in the transmission of 'bison type' genotype of mycobacterium avium subspecies paratuberculosis in india.information on mycobacterium avium subspecies paratuberculosis (map) genotypes infecting different animal species in india is limited. presence of map was investigated in free ranging antelopes (locally known as nilgai/blue bulls/boselaphus tragocamelus) using direct microscopy, culture, is900 pcr and is1311 pcr-rea. is900 elements of map from nilgai and previously isolated from goats were sequenced and compared to establish inter-species transmission between free ranging nilgai and closed farm ...201018805585
presence and characterization of mycobacterium avium subspecies paratuberculosis from clinical and suspected cases of crohn's disease and in the healthy human population in india.to investigate and characterize mycobacterium avium subspecies paratuberculosis (map) in patients with crohn's disease, attendants of animals with suspected infection, and healthy humans, using multiple diagnostic tests.200817913536
pathogenic 'bison-type' mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (bubalus bubalis) in north india.despite low per-animal productivity of ruminants in developing countries, johne's disease has not been investigated in buffaloes, which are primarily found in these countries. this is due to lack of expertise, diagnostic kits and priority to production diseases like johne's disease. presence of pathogenic mycobacterium avium subspecies paratuberculosis (map) was investigated by screening of target tissues (mesenteric lymph nodes and large intestine) by culture and is 900 pcr, in 50 sacrificed bu ...200817804069
sero-prevalence of bovine johne's disease in buffaloes and cattle population of north india using indigenous elisa kit based on native mycobacterium avium subspecies paratuberculosis 'bison type' genotype of goat origin.present pilot study is the first attempt in the country to estimate sero-prevalence of bovine johne's disease (bjd) by screening cattle and buffaloes representing large population belonging to farmer's and farm herds in the home tracts (uttar pradesh (up) and punjab) of hariana cattle and murrah buffaloes in north india. indigenous and in-house plate elisa kit (using protoplasmic antigen from native mycobacterium avium subsp. paratuberculosis 'bison type' strain of goat origin), originally devel ...200817854892
molecular identification and characterization of mycobacterium avium subspecies paratuberculosis in free living non-human primate (rhesus macaques) from north india.in recent years, mycobacterium avium subspecies paratuberculosis (map) has emerged as major animal pathogen with significant zoonotic concerns, worldwide. map infection is endemic in domestic and wild ruminant population in india. however, information on map infection in free ranging animal species and non human primates is limited. present study aimed to estimate the status of map infection in free living rhesus macaques suffering with multiple clinical conditions (coughing and loose stool). a ...201121255839
genotype profiles of mycobacterium avium subspecies paratuberculosis isolates recovered from animals, commercial milk, and human beings in north india.to understand the genetic diversity of mycobacterium avium subspecies paratuberculosis (map) isolates recovered from domestic and wild ruminants, commercial milk, and human beings in north india.200919233699
evaluation of indigenous milk elisa with m-culture and m-pcr for the diagnosis of bovine johne's disease (bjd) in lactating indian dairy cattle.present study is the first attempt to evaluate an indigenous milk elisa with milk culture, standardize milk pcr, estimate lacto-prevalence of map and genotype map dna from milk samples in few indian dairy herds. in all 115 cows were sampled from 669 lactating cows in six dairy herds from three districts of north india. fifty milk samples (four herds) were screened by three tests (milk culture, m-elisa and m-pcr). lacto-prevalence of map in four dairy herds was 84.0% (50.0% in fat and 62.0% in se ...200817544046
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