| characterization of cmy-type beta-lactamases in clinical strains of proteus mirabilis and klebsiella pneumoniae isolated in four hospitals in the paris area. | we isolated five clinical strains (three proteus mirabilis and two klebsiella pneumoniae) with beta-lactam resistance phenotypes consistent with production of an ampc-type beta-lactamase. the predicted amino acid sequences of the enzymes were typical of class c beta-lactamases. the enzymes were identified as cmy-2, cmy-4 and a new cmy-variant beta-lactamase, cmy-12. the ampc beta-lactamases from the two k. pneumoniae isolates were found to be encoded on self-transferable plasmids. the genes enco ... | 2002 | 12407124 |
| occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005. | bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ... | 2006 | 16961639 |
| multidrug-resistant bacteria in hospitalized children: a 5-year multicenter study. | the objective of this study was to determine the incidence of multidrug-resistant bacteria in hospitalized children. | 2007 | 17403822 |
| [implication of extended-spectrum beta-lactamase enterobacteriaceae in nosocomial infections in neonates]. | the objective of this study was to determine the incidence of extended-spectrum beta-lactamase (esbls) enterobacteriaceae colonization and infection in hospitalized children. | 2010 | 20826324 |
| veterinary hospital-acquired infections in pets with a ciprofloxacin-resistant ctx-m-15-producing klebsiella pneumoniae st15 clone. | | 2011 | 22178643 |
| dissemination of ctx-m-type beta-lactamases among clinical isolates of enterobacteriaceae in paris, france. | we analyzed 19 clinical isolates of the family enterobacteriaceae (16 escherichia coli isolates and 3 klebsiella pneumoniae isolates) collected from four different hospitals in paris, france, from 2000 to 2002. these strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. the bla(ctx-m) genes encoding these beta-lactamases were involved in this resistance, with a predominance of bla ... | 2004 | 15047527 |
| clinical and bacteriologic epidemiology of extended-spectrum beta-lactamase-producing strains of klebsiella pneumoniae in a medical intensive care unit. | the epidemiology of extended-spectrum beta-lactamase (esbl)-producing strains of klebsiella pneumoniae was studied over a 16-month period in a medical intensive care unit (icu). a control program involving enhanced isolation procedures, surveillance cultures at admission and then at 1-week intervals, and selective digestive decontamination (sdd) was instituted. phenotypic and genotypic markers (plasmid content and dna macrorestriction polymorphism determined by pulsed-field gel electrophoresis) ... | 1998 | 9798042 |
| secondary carriage with multi-resistant acinetobacter baumannii and klebsiella pneumoniae in an adult icu population: relationship with nosocomial infections and mortality. | a one year prospective, observational survey was performed to evaluate the abnormal carriage of multi-resistant klebsiella pneumoniae and/ or acinetobacter baumannii, to determine associated risk factors for carriage, and to correlate the abnormal carriage with infectious morbidity and mortality in the intensive care unit (icu) of a university hospital. two hundred and ninety-eight patients who stayed in the icu > 48h, and were not neutropenic, were studied. salivary and rectal samples were obta ... | 1996 | 8971617 |
| molecular epidemiology of plasmid spread among extended broad-spectrum beta-lactamase-producing klebsiella pneumoniae isolates in a pediatric hospital. | over a 12-month period, 43 children in eight different wards of our hospital (hôpital robert debré) were infected or colonized with klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. the epidemiology of the outbreak was studied by a molecular approach including the determination of the beta-lactamase physicochemical parameters and plasmid profiles, as well as analysis of the restriction fragment length polymorphisms of the rdna regions (ribotyping). the last approac ... | 1993 | 8432800 |
| dissemination in five french hospitals of klebsiella pneumoniae serotype k25 harbouring a new transferable enzymatic resistance to third generation cephalosporins and aztreonam. | a strain of klebsiella pneumoniae k25 resistant to newer beta-lactam drugs was isolated in clusters in five hospitals in the paris area. the mics of ceftazidime and aztreonam (greater than or equal to 128 mg/l) were higher than that of cefotaxime (16 mg/l) for the strain but, when measured in the presence of clavulanic acid, they were less than or equal to 1 mg/l. the donor strains and derivatives produced a beta-lactamase with a pi of 7.75-7.8 and hydrolysing activity against a wide spectrum of ... | 1988 | 3145863 |
| transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant klebsiella pneumoniae. | klebsiella pneumoniae strains that were resistant to third-generation cephalosporins and amikacin were recovered from 62 of 395 patients (15.7%) during 1986. 25 isolates (40%) caused urinary tract infections. the outbreak involved three intensive care units (54 isolates), and spread from one unit to another and then to four wards (8 isolates). k pneumoniae of various serotypes and strains of different enterobacteriaceae demonstrating the same antibiotic resistance pattern were isolated, which su ... | 1987 | 2886766 |
| intestinal decontamination for control of nosocomial multiresistant gram-negative bacilli. study of an outbreak in an intensive care unit. | to study the efficacy of intestinal decontamination by oral nonabsorbable antibiotic agents to control a nosocomial outbreak of intestinal colonization and infection with multiresistant enterobacteriaceae, and to examine its effects on endemic nosocomial infection rates. | 1989 | 2655521 |
| outbreak of nosocomial infections due to klebsiella pneumoniae producing shv-4 beta-lactamase. | one hundred and fifty-four clinical isolates of klebsiella pneumoniae resistant to broad-spectrum cephalosporins, aztreonam and amikacin were responsible for an outbreak of nosocomial infections lasting eight months in a university hospital in paris. this outbreak occurred in the intensive care unit (39 patients), haematology units (8 patients) and surgical and medical units (11 patients). antibiotic resistant strains were isolated from the urinary tract (48%), wound and drainage fluids (21%), r ... | 1990 | 2086215 |
| high prevalence of blactx-m-1/inci1/st3 and blacmy-2/inci1/st2 plasmids in healthy urban dogs in france. | in the community, close contacts between humans and dogs may promote the transfer of extended-spectrum beta-lactamase/plasmidic ampc cephalosporinase (esbl/pampc) genes. large-scale prevalence studies on esbl/pampc carriage in dogs are rare, and data on esbl/pampc plasmids are even more limited. here, a considerable rate of 18.5% esbl/pampc carriers was found among 368 unrelated healthy dogs in paris, france. this prevalence is much higher than the one found in healthy humans in the same city (6 ... | 2014 | 24982072 |
| clinical and microbiological determinants of severe and fatal outcomes in patients infected with enterobacteriaceae producing extended-spectrum β-lactamase. | although extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae have become a worldwide public health concern, little is known regarding the clinical course of colonized or infected individuals. our objective was to characterize the determinants of fatal outcomes related to esbl-producing microorganisms at a large hospital in paris, france. in 2012-2013, all consecutive patients with clinical samples testing positive for esbl-producing enterobacteriaceae at saint-antoine hospital were ... | 2017 | 28181033 |
| extended-spectrum β-lactamase ctx-m-15-producing klebsiella pneumoniae of sequence type st274 in companion animals. | screening of extended-spectrum β-lactamase (esbl)-producing gram-negative bacteria in companion animals living in the paris area in france identified a high rate of ctx-m-15-producing klebsiella pneumoniae. those isolates were recovered during the 2010-2011 period from both infections and asymptomatic colonizations. sequence typing revealed that most of these isolates belonged to sequence type st274. interestingly, the bla(ctx-m-15) gene was located on a specific and novel plasmid scaffold. thes ... | 2013 | 23422912 |
| [phenotypic detection of carbapenemase associated with extended-spectrum beta-lactamase in klebsiella pneumoniae]. | | 2011 | 22154523 |
| outbreak of oxa-48-positive carbapenem-resistant klebsiella pneumoniae isolates in france. | seventeen klebsiella pneumoniae isolates producing the oxa-48 carbapenemase, obtained from 10 patients hospitalized from april to june 2010, mostly in the medical intensive care unit of the villeneuve-saint-georges hospital in a suburb of paris, france, were analyzed. seven patients were infected, of whom five were treated at least with a carbapenem, and five patients died. molecular analysis showed that the isolates belonged to a single clone that harbored a 70-kb plasmid carrying the blaoxa-48 ... | 2011 | 21343451 |
| control of a multi-hospital outbreak of kpc-producing klebsiella pneumoniae type 2 in france, september to october 2009. | an outbreak of klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae type 2 was detected in september 2009 in two hospitals in a suburb south of paris, france. in total, 13 kpc-producing k. pneumoniae type 2 cases (four with infections and nine with digestive-tract colonisations) were identified, including a source case transferred from a greek hospital. of the 13 cases, seven were secondary cases associated with use of a contaminated duodenoscope used to examine the source c ... | 2010 | 21144448 |
| outbreak of ctx-m-15-producing klebsiella pneumoniae in the intensive care unit of a french hospital. | the ctx-m-15 extended spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae isolates were identified in 36 patients hospitalized from december 2006 to september 2007 in the medical intensive care unit (icu) of the bicêtre hospital, south paris, france. the incidence of colonization and/or infection was 4.8%. eighty-nine percent of the esbl-producing k. pneumoniae isolates were acquired in the icu, and only 8.3% of the patients were infected. pulsed field gel electrophoresis (pfge) analy ... | 2009 | 19231938 |
| the emergence of multidrug-resistant klebsiella pneumoniae of international clones st13, st16, st35, st48 and st101 in a teaching hospital in the paris region. | despite infection control measures, an important increase in the extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae incidence density occurred in our hospital from 2006 onwards. this study, focusing on the 2005-2007 period, was performed in an attempt to explain this increase. esbls were characterized, isolates were typed by eric2-pcr, and sequence type (st) of clustered isolates was determined. temporal-spatial relationships of patients were analysed to assess possible cross-c ... | 2013 | 23034125 |