investigation of a listeriosis epizootic in sheep in new york investigate potential sources of an epizootic of listerial encephalitis, using molecular diagnostic and typing methods.19979215449
risk factors associated with salmonella and listeria monocytogenes contamination of produce fields.identification of management practices associated with preharvest pathogen contamination of produce fields is crucial to the development of effective good agricultural practices (gaps). a cross-sectional study was conducted to (i) determine management practices associated with a salmonella- or listeria monocytogenes-positive field and (ii) quantify the frequency of these pathogens in irrigation and nonirrigation water sources. over 5 weeks, 21 produce farms in new york state were visited. field- ...201324077713
geographical and meteorological factors associated with isolation of listeria species in new york state produce production and natural environments.listeria species have been isolated from diverse environments, often at considerable prevalence, and are known to persist in food processing facilities. the presence of listeria spp. has been suggested to be a marker for listeria monocytogenes contamination. therefore, a study was conducted to (i) determine the prevalence and diversity of listeria spp. in produce production and natural environments and (ii) identify geographical and/or meteorological factors that affect the isolation of listeria ...201425364926
spatial and temporal factors associated with an increased prevalence of listeria monocytogenes in spinach fields in new york state.while rain and irrigation events have been associated with an increased prevalence of foodborne pathogens in produce production environments, quantitative data are needed to determine the effects of various spatial and temporal factors on the risk of produce contamination following these events. this study was performed to quantify these effects and to determine the impact of rain and irrigation events on the detection frequency and diversity of listeria species (including l. monocytogenes) and ...201526116668
listeria monocytogenes infections: laboratory investigation of an unexpected increase in incidence.listeria monocytogenes is a facultative intracellular pathogen causing mainly meningitis and septicemia in immunocompromised hosts. from july 1988 through december 1989, 16 patients at the mount sinai hospital were diagnosed as having listeriosis shortly after admission, 14 within a one-year period (july 1988-july 1989). because this incidence was almost double the incidence in previous years (< 8 annually), an epidemiologic and microbiologic investigation was undertaken to determine a potential ...19957616977
molecular ecology of listeria monocytogenes: evidence for a reservoir in milking equipment on a dairy farm.a longitudinal study aimed to detect listeria monocytogenes on a new york state dairy farm was conducted between february 2004 and july 2007. fecal samples were collected every 6 months from all lactating cows. approximately 20 environmental samples were obtained every 3 months. bulk tank milk samples and in-line milk filter samples were obtained weekly. samples from milking equipment and the milking parlor environment were obtained in may 2007. fifty-one of 715 fecal samples (7.1%) and 22 of 30 ...200919114514
low prevalence of listeria monocytogenes in human stool.listeria monocytogenes is a foodborne pathogen that is found widely in the environment and in a variety of ready-to-eat foods, yet human invasive infection is relatively rare (five cases per million people annually in the united states). despite wide exposure to this organism, little is known about the prevalence of l. monocytogenes in human stool, and it is not known whether human fecal dispersal contributes to human foodborne transmission. we cultured 827 stool specimens (well formed and loose ...200515690823
multistate outbreak of listeriosis--united states, 1998.since early august 1998, 40 illnesses caused by a single strain of listeria monocytogenes (lm) have been identified in 10 states: ohio (13 cases); new york; tennessee, massachusetts, and west virginia (three each); michigan (two); and connecticut, oregon, vermont, and georgia (one each). dates of illness onset or lm isolation ranged from august 2 through december 2. all lm isolates from these cases are serotype 4b and share an unusual pattern when subtyped either by pulsed-field gel electrophore ...19989883769
diagnosis and epidemiological association of listeria monocytogenes strains in two outbreaks of listerial encephalitis in small ruminants.two outbreaks of epizootic listerial encephalitis, one in sheep and one in goats, were investigated through pathology, microbiology, and dna amplification-based techniques. efforts were made to survey the diversity of listeria monocytogenes strains in the silage consumed by affected animals and to verify the causal relationship between silage and disease outbreak. in both outbreaks, l. monocytogenes was isolated from silage and brain tissue samples. random amplified polymorphic dna patterns reve ...19948027356
multistate outbreak of listeriosis--united states, 2000.since may 2000, 29 illnesses caused by a strain of listeria monocytogenes (lm) have been identified in 10 states: new york (15 cases); georgia (three); connecticut, ohio, and michigan (two each); and california, pennsylvania, tennessee, utah, and wisconsin (one each). dates of lm isolation ranged from may 17 through november 26 with 26 (90%) infections occurring since july 15. when subtyped, the lm isolates from these cases were indistinguishable by pulsed-field gel electrophoresis (pulsenet pat ...200011190115
identification of potential on-farm sources of listeria monocytogenes in herds of dairy elucidate the ecology of listeria monocytogenes on dairy cattle farms by determining the prevalence of the organism in various samples.200919254151
molecular epidemiology and cluster analysis of human listeriosis cases in three u.s. better understand the transmission and epidemiology of human listeriosis, 647 listeria monocytogenes isolates obtained from human listeriosis cases in four u.s. locations (michigan, ohio, new york state, and new york city) over 61 months (1998 to 2003) were characterized by automated ecori ribotyping. a total of 65 ribotypes were differentiated among the characterized isolates; 393, 227, and 24 isolates were classified into lineages i, ii, and iii, respectively, and 3 isolates were not classi ...200616865904
microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the united states to contaminated hot dogs and establishment of molecular subtyping-based surveillance for listeria monocytogenes in the pulsenet network.a multistate outbreak of listeriosis occurred in the united states in 1998 with illness onset dates between august and december. the outbreak caused illness in 108 persons residing in 24 states and caused 14 deaths and four miscarriages or stillbirths. this outbreak was detected by public health officials in tennessee and new york who observed significant increases over expected listeriosis cases in their states. subsequently, the centers for disease control and prevention (cdc) began laboratory ...200515872265
a cross-sectional study on the prevalence of listeria monocytogenes and salmonella in new york dairy part of our long-term objective of assessing risk for listeria monocytogenes and salmonella spp. in dairy herds, we carried out a cross-sectional study to determine the prevalence of the two organisms. the study population consisted of a sample of dairy herds enrolled in the quality milk promotion services at cornell during the period of april 1998 to march 1999. the sample was stratified by geographical region to assure representation. four hundred and four dairy farms were enrolled in the s ...200011104262
molecular subtyping to detect human listeriosis clusters.we analyzed the diversity (simpson's index, d) and distribution of listeria monocytogenes in human listeriosis cases in new york state (excluding new york city) from november 1996 to june 2000 by using automated ribotyping and pulsed-field gel electrophoresis (pfge). we applied a scan statistic (p<or=0.05) to detect listeriosis clusters caused by a specific listeria monocytogenes subtype. among 131 human isolates, 34 (d=0.923) ribotypes and 74 (d=0.975) pfge types were found. nine (31% of cases) ...200312781006
distribution of listeria monocytogenes molecular subtypes among human and food isolates from new york state shows persistence of human disease--associated listeria monocytogenes strains in retail environments.while there is considerable information available regarding listeria monocytogenes contamination patterns in food processing plants, our understanding of l. monocytogenes contamination and transmission in retail operations is limited. we characterized 125 food, 40 environmental, and 342 human clinical l. monocytogenes isolates collected in new york state from 1997 to 2002 using automated ribotyping and hly allelic variation. all environmental isolates were obtained from retail establishments and ...200415270495
validation of a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in new york state produce fields.technological advancements, particularly in the field of geographic information systems (gis), have made it possible to predict the likelihood of foodborne pathogen contamination in produce production environments using geospatial models. yet, few studies have examined the validity and robustness of such models. this study was performed to test and refine the rules associated with a previously developed geospatial model that predicts the prevalence of listeria monocytogenes in produce farms in n ...201526590280
outbreak of listeriosis--northeastern united states, 2002.a multistate outbreak of listeria monocytogenes infections with 46 culture-confirmed cases, seven deaths, and three stillbirths or miscarriages in eight states has been linked to eating sliceable turkey deli meat. cases have been reported from pennsylvania (14 cases), new york (11 in new york city and seven in other locations), new jersey (five), delaware (four), maryland (two), connecticut (one), massachusetts (one), and michigan (one). culture dates ranged from july 18 to september 30, 2002; c ...200212437035
landscape and meteorological factors affecting prevalence of three food-borne pathogens in fruit and vegetable farms.produce-related outbreaks have been traced back to the preharvest environment. a longitudinal study was conducted on five farms in new york state to characterize the prevalence, persistence, and diversity of food-borne pathogens in fresh produce fields and to determine landscape and meteorological factors that predict their presence. produce fields were sampled four times per year for 2 years. a total of 588 samples were analyzed for listeria monocytogenes, salmonella, and shiga toxin-producing ...201323144137
listeria monocytogenes fecal shedding in dairy cattle shows high levels of day-to-day variation and includes outbreaks and sporadic cases of shedding of specific l. monocytogenes subtypes.fecal shedding of listeria monocytogenes poses a risk for contamination of animal feed and agricultural environments and raw food at the pre-harvest stages of food production. to be able to reduce these risks it is critical to improve understanding of the epidemiology of l. monocytogenes shedding in feces. the objective of this study was to assess the daily variability of fecal shedding and its association with individual animal (lactation number and the day of current lactation) and environment ...200717481754
farm-management and milking practices associated with the presence of listeria monocytogenes in new york state dairy herds.knowledge of the relationship between dairy herd management and milking practices with the occurrence of listeria monocytogenes on dairy farms might assist in the development of intervention strategies aimed at eliminating this organism at the pre-harvest dairy-food-production level. this paper represents a first step towards that goal. we carried out a cross-sectional study to identify farm factors that were associated with isolation of l. monocytogenes from on-farm in-line milk-filters. data o ...200111530195
listeriosis in seven bushy-tailed jirds.listeriosis was diagnosed in 7 bushy-tailed jirds (sekeetamys calurus). death in all cases was acute, and there were no clinical signs. necropsy revealed necrotizing lesions in the liver, intestines, spleen, and hepatic and mesenteric lymph nodes. on histologic examination large numbers of gram-positive coccobacilli were seen in the lesions. pneumonitis was seen in 6 jirds, and small numbers of bacteria were seen in the lungs in all cases except one. diagnosis was based on bacterial culture of t ...19846511584
multistate outbreak of listeria monocytogenes infection linked to delicatessen turkey meat.despite a decreasing incidence of listeriosis in the united states, molecular subtyping has increased the number of recognized outbreaks. in september 2000, the new york city department of health identified a cluster of infections caused by listeria monocytogenes isolates with identical molecular subtypes by pulsed-field gel electrophoresis (pfge) and ribotyping.200515824987
central nervous system infections in patients with cancer. changing patterns.central nervous system infections in patients with cancer at memorial sloan-kettering cancer center from 1971 to 1974 were surveyed and compared with a previous survey from 1955 to 1970. the two periods were similar in that: 1) there was a high incidence of cns infection in patients with lymphoma, leukemia, and head and spine tumors, 2) specific organisms tended to infect patients with certain primary neoplasms, and 3) fungal infections were common and difficult to diagnose. the two periods diff ...1977880555
irrigation is significantly associated with an increased prevalence of listeria monocytogenes in produce production environments in new york state.environmental (i.e., meteorological and landscape) factors and management practices can affect the prevalence of foodborne pathogens in produce production environments. this study was conducted to determine the prevalence of listeria monocytogenes, listeria species (including l. monocytogenes), salmonella, and shiga toxin-producing escherichia coli (stec) in produce production environments and to identify environmental factors and management practices associated with their isolation. ten produce ...201526038903
recurrent listeria monocytogenes infection: relapse or reinfection with a unique strain confirmed by molecular subtyping.we report a case of recurrent listeriosis for which molecular subtyping by automated ribotyping and pulsed-field gel electrophoresis confirmed either relapse of infection or reinfection due to a common source almost 9 months after initial infection due to a unique listeria monocytogenes strain in a patient with colorectal cancer. this case report illustrates the potential use of molecular subtyping to further understand the pathogenesis and epidemiology of listeriosis and the potential for relap ...200111418887
a multiplex pcr/ldr assay for simultaneous detection and identification of the niaid category b bacterial food and water-borne pathogens.enteric pathogens that cause gastroenteritis remain a major global health concern. the goal of this study was to develop a multiplex pcr/ligation detection reaction (ldr) assay for the detection of all niaid category b bacterial food and water-borne pathogens directly from stool specimens. to validate the pcr/ldr assay, clinical isolates of campylobacter spp., vibrio spp., shigella spp., salmonella spp., listeria monocytogenes, yersinia enterocolitica, and diarrheagenic escherichia coli were tes ...201424709368
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