incidence of listeria monocytogenes in cheese manufacturing plants from the northeast region of são paulo, brazil.the incidence of listeria monocytogenes in three cheese manufacturing plants from the northeastern region of são paulo, brazil, was evaluated from october 2008 to september 2009. l. monocytogenes was found in samples from two plants, at percentages of 13.3% (n = 128) and 9.6% (n = 114). samples of raw and pasteurized milk, water, and minas frescal cheese were negative for l. monocytogenes, although the pathogen was isolated from the surface of prato cheese and in brine from one of the plants eva ...201121549054
risky consumption habits and safety of fluid milk available in retail sales outlets in viçosa, minas gerais state, brazil.this study aimed to assess raw milk consumption habits in the urban population of viçosa, minas gerais, brazil, and the microbiological safety and quality of the fluid milk available in retail sales outlets in the same region. a simplified questionnaire regarding raw milk consumption was applied to the persons responsible for food acquisition in 411 residences. the regular consumption of raw milk was observed by 18.5% of the interviewers, and lack of knowledge of possible risks related to this f ...201424697652
microbiological aspects of the biofilm on wooden utensils used to make a brazilian artisanal cheese.the artisanal minas cheese is produced from raw cow's milk and wooden utensils were employed in its manufacture, which were replaced by other materials at the request of local laws. this substitution caused changes in the traditional characteristics of cheese. due to the absence of scientific studies indicating the microbial composition of biofilms formed on wooden forms, tables and shelves used in these cheese production, the present work evaluated the counts of staphylococcus aureus, escherich ...201425242963
determining the minimum ripening time of artisanal minas cheese, a traditional brazilian cheese.physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal minas cheese from eight producers from serro region (minas gerais, brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by brazilian legislation. the cheeses were produced between dry season (april-september) and rainy season (october-march); 128 cheeses were ripened at room temperature (25 ± 4 °c), and 128 were ri ...201526221111
yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil.the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ...201324516436
bacteriological quality of raw milk used for production of a brazilian farmstead raw milk cheese.abstract the objective of this study was to evaluate the bacteriological quality of raw cow's milk utilized for the production of traditional minas serro cheese, a brazilian farmstead raw milk cheese. raw milk samples were collected from six farmstead cheese operations manufacturing raw milk cheese from cow's milk. coliform count (cc) and escherichia coli counts were determined using petrifilm(™) ec plates, and staphylococcus aureus counts were determined using petrifilm(™) staph express count ...201222217108
incidence of listeria monocytogenes in cheese produced in rio de janeiro, brazil.the present study evaluated the incidence of listeria spp. in some brazilian cheeses obtained from retail stores in rio de janeiro, of 103 samples of various types of cheese examined as recommended in the listeria isolation protocol of the health protection branch of canada, 11 (10.68%) were contaminated by listeria monocytogenes, 13 (12.62%) by listeria innocua, 6 (5.83%) by listeria grayi, and 1 (0.97%) by listeria welshimeri. a higher incidence of l. monocytogenes as observed mainly in the ho ...19989708310
[occurrence of listeria monocytogenes in raw milk, pasteurized type c milk and minas frescal cheese commercialized in piracicaba-são paulo].samples of raw milk, pasteurized c type milk and minas frescal cheese were analyzed for the presence of listeria monocytogenes by a method developed for dairy products by lovett (1) and by lovett & hitchins (2). a total of 20 samples of each product and from different commercial brands were analyzed, obtained from various retail stores in piracicaba, sp. according to the pattern of sampling and methodology adopted, none of the samples of the aforesaid products was positive for the presence of l. ...19947786098
using nested pcr to detect the hlya gene of listeria monocytogenes in minas frescal cow's milk evaluate a nested pcr protocol for listeria monocytogenes detection, minas frescal cow's milk cheeses were produced, artificially inoculated with this pathogen at concentrations ranging from 1 to 1,000 cfu/g, and stored at 4°c for 10 days. the international organization for standardization (iso) standardized method 11290-1/a1 was used to detect l. monocytogenes in the inoculated samples, and dna was extracted from aliquots (1, 5, and 10 ml) of 1:10 dilution, followed by a nested pcr protocol ...201222980018
occurrence of salmonella, listeria monocytogenes, and enterotoxigenic staphylococcus in goat milk from small and medium-sized farms located in minas gerais state, brazil.consumption of goat milk has been increasing due to its nutritional characteristics and health benefits. therefore, assessment of the presence of foodborne pathogens in this product is critical to ensure its safety to consumers. the present study aimed to identify common foodborne pathogens in raw goat milk. fifty-three samples of raw goat milk from 11 farms were collected and cultured for the presence of salmonella spp. and listeria monocytogenes, as well as for enumeration and isolation of coa ...201526476939
retail survey of brazilian milk and minas frescal cheese and a contaminated dairy plant to establish prevalence, relatedness, and sources of listeria monocytogenes isolates.a study was designed to recover listeria monocytogenes from pasteurized milk and minas frescal cheese (mfc) sampled at retail establishments (res) and to identify the contamination source(s) of these products in the corresponding dairy processing plant. fifty milk samples (9 brands) and 55 mfc samples (10 brands) were tested from res located in juiz de fora, minas gerais, brazil. all milk samples and 45 samples from 9 of 10 mfc brands tested negative for l. monocytogenes; however, "brand f" of m ...200818502929
occurrence of listeria spp. in critical control points and the environment of minas frescal cheese processing.critical control points (ccps) associated with minas frescal cheese (a brazilian soft white cheese, eaten fresh) processing in two dairy factories were determined using flow diagrams and microbiological tests for detection of listeria monocytogenes and other species of listeria. a total of 218 samples were collected along the production line and environment. the ccps identified were reception of raw milk, pasteurization, coagulation and storage. thirteen samples were positive for listeria; 9 sam ...200312485750
pulsed-field gel electrophoresis characterization of listeria monocytogenes isolates from cheese manufacturing plants in são paulo, brazil.this study aimed to evaluate the occurrence of listeria monocytogenes in cheese and in the environment of three small-scale dairy plants (a, b, c) located in the northern region state of são paulo, brazil, and to characterize the isolates using conventional serotyping and pfge. a total of 393 samples were collected and analyzed from october 2008 to september 2009. from these, 136 came from dairy plant a, where only l. seeligeri was isolated. in dairy plant b, 136 samples were analyzed, and l. in ...201424412413
low occurrence of listeria monocytogenes on bovine hides and carcasses in minas gerais state, brazil: molecular characterization and antimicrobial resistance.listeria monocytogenes occurrence was assessed in three slaughterhouses located in minas gerais state, brazil, by analysis of 209 bovine carcasses. four sponge samples were obtained from each carcass in different steps (a, from hide, before bleeding; b, after hide removal; c, after evisceration; and d, after end washing), resulting in a total of 836 samples. the samples were tested for the presence of l. monocytogenes according to the international organization for standardization 11290-1, and p ...201424988021
identification and evaluation of the probiotic potential of lactobacillus paraplantarum ft259, a bacteriocinogenic strain isolated from brazilian semi-hard artisanal cheese.this study aimed to identify a bacteriocinogenic lactobacillus isolate (ft259) obtained from brazilian semi-hard minas type cheese and to evaluate its probiotic and antimicrobial potentials. the strain was identified by biochemical tests (at genus level), and by 16s rdna sequencing combined with reca gene amplification (for species). to determine the inhibitory spectrum towards food borne pathogens and lactic acid bacteria, the spot-on-the-lawn assay was carried out. moreover, the proteinaceous ...201323792229
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