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characterization of listeria monocytogenes isolates from human listeriosis cases in italy.the objective of this study was to characterize by serotyping, pulsed-field gel electrophoresis (pfge), and pcr amplification of virulence genes and markers of epidemic clones i, ii, and iii (eci, ecii, and eciii) 54 human isolates from apparently sporadic cases of infection occurring in the lombardy region and in the province of florence, tuscany, italy, in the years 1996 to 2007. listeria monocytogenes isolates were provided by the clinical microbiology laboratories of the lombardy region and ...200919605584
molecular typing reveals frequent clustering among human isolates of listeria monocytogenes in italy.in italy, the annual incidence of reported cases of listeriosis amounts in recent years (2004 to 2006) to 0.8 case per million inhabitants. our study is a subtyping analysis by serotyping, ribotyping, and pulsed-field gel electrophoresis analysis of 44 human isolates from apparently sporadic cases of infection in the lombardy region and in the province of florence, italy, in the years 1996 to 2007. based on the results of the different subtyping methods, 10 occasions were detected when strains o ...200919435242
microbial composition, including the incidence of pathogens, of goat milk from the bergamo region of italy during a lactation year.sixty samples of raw goat milk intended for caprino cheese-making were collected from ten farms in the bergamo area over a 6-month period. analyses of main microbial groups, somatic cell count (scc) and ph were performed to determine the effect of origin (farm) and lactation period (april - september) on microbial composition and the incidence of pathogens in milk. overall mean values were: standard plate count (spc), 5.0 x 10(4) cfu/ml; yeasts, 2.5 x 10(2) cfu/ml; coliforms, 91 x 10(2) cfu/ml; ...200212222800
diversity and persistence of listeria monocytogenes within the gorgonzola pdo production chain and comparison with clinical isolates from the same area.listeria monocytogenes causes invasive syndromes with high fatality rates in specific population groups. cheeses have been commonly implicated in outbreaks worldwide. gorgonzola is a cheese only produced in northwestern italy (it is the third italian cheese in terms of production and export) and l. monocytogenes is frequently isolated from the production chain. the aims of this study were to assess the distribution of l. monocytogenes virulence types (vts) in isolates collected in gorgonzola pro ...201728153561
occurrence of hepatitis a and e and norovirus gi and gii in ready-to-eat vegetables in italy.fresh vegetables and their ready-to-eat (rte) salads have become increasingly recognized as potential vehicles for foodborne diseases. the eu reg. 1441/2007 establishes microbiological criteria for bacterial pathogens for products placed on the market during their shelf-life (i.e. salmonella spp., listeria monocytogenes) for pre-cut fruits and vegetables (rte) whilst it does not address the problem of contamination by enteric viruses. in this study we investigated the contamination by hepatitis ...201728319799
identification of a major listeria monocytogenes outbreak clone linked to soft cheese in northern italy - 2009-2011.molecular subtyping and enhanced surveillance in lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. this cluster grouped 31 isolates that belonged to serotype 1/2a and sequence type 38 (st38) as defined by multilocus sequence typing (mlst).201728499357
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