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characterization of specific alleles in inla and prfa of listeria monocytogenes isolated from foods in osaka, japan and their ability to invade caco-2 cells.listeria monocytogenes expresses the surface protein internalin a (inla), enabling the invasion of human intestinal epithelial cells to cause severe food-borne diseases. full-length sequence analysis of inla of 114 food isolates resulted in the detection of 29 isolates with a premature stop codon (pmsc) mutation and 6 isolates with 3-codon deletion mutations (aa 738 to 740) in inla. the isolates with inla pmscs demonstrated a significantly lower level of invasion than the other food isolates in ...201526143289
molecular typing to trace listeria monocytogenes isolated from cold-smoked fish to a contamination source in a processing plant.in this study, listeria monocytogenes contamination in a cold-smoked fish processing plant in osaka, japan, was examined from 2002 to 2004. a total of 430 samples were collected and divided into five categories: raw fish, materials during processing, processing equipment, environment, and finished products. a total of 59 finished products were examined throughout this study. l. monocytogenes was isolated from four of these samples during summer and autumn but was not found during winter or sprin ...200616629026
listeria monocytogenes isolated from cold-smoked fish products in osaka city, japan.listeria monocytogenes contamination of ready-to-eat seafood products commercially available in osaka was examined between 1999 and 2000. l. monocytogenes was isolated from 12 (13%) of the 95 products tested. all positive samples were from cold-smoked fish with 9 being obtained during the summer. thirteen isolates of l. monocytogenes were typed by pulsed-field gel electrophoresis (pfge) and polymerase chain reaction (pcr)-based typing methods. isolates of the same serotype originating from the s ...200415246243
relationship between bacterial meningitis and acellular pertussis vaccine.we studied case reports of bacterial meningitis and estimated its incidence per 100,000 in three areas of osaka prefecture which differed in population and vaccination schedule for the years of 1987 and 1988. to estimate incidence, we used a simple mathematical method derived from surveillance data for a reported number of exanthem subitum to obtain a coverage constant. with this coverage constant, we estimated the incidence of bacterial meningitis per 100,000 population for each area. in the to ...19911778325
[two cases of listeria monocytogenes infection in osaka municipal hospital].we report two cases of sepsis and meningoencephalitis with listeriosis. they died in despite of administering sensitive antibiotics. a 2 day old girl was admitted to our hospital because of fever and cyanosis. listeria monocytogenes type 4b was cultured from blood, csf, throat, urine, ear. she was treated with twice exchange transfusion and sensitive antibiotics (abpc, tob), but died from dic. a 48 year old man suddenly experienced an unconscious condition. a csf culture grew l. monocytogenes ty ...19968741712
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