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comparison of indirect fluorescent antibody test (ifat) and slide enzyme linked immunosorbent assay (selisa) for diagnosis of babesia bigemina infection in bovines.an indirect fluorescent antibody test (ifat) and slide enzyme linked immunosorbent assay (selisa) were standardized for the detection of antibodies specific to babesia bigemina in experimentally infected bovine calves and subsequently used for the screening of naturally infected bovine and bubaline sera. in experimentally infected calves positive reactivity was detected in sera at the earliest on day 7 by both the tests. serological studies for detection of b. bigemina specific antibodies in 180 ...200919115088
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