| four patients in singapore with anti-golgi antibodies. | serum specimens for anti-nuclear fluorescence tests are routinely received in our laboratory. four specimens were spotted to be negative for anti-nuclear fluorescence but positive for fluorescence characteristic of that caused by anti-golgi antibodies. (a) patient a had acute glomerulonephritis; (b) patient b had acute viral hepatitis; (c) patient c had deep vein thrombosis; and (d) patient d had non-hodgkin's lymphoma. the relevance and possible aetiology of anti-golgi antibodies are also discu ... | 1991 | 1788578 |
| hepatitis c virus genotypes in singapore and indonesia. | 5' untranslated and partial core (c) region sequence of hepatitis c virus (hcv) in 21 singaporean and 15 indonesian isolates were amplified by reverse-transcription polymerase chain reaction and sequenced with the use of conserved primer sequences deduced from hcv genomes identified in other geographical regions. the hcv genotypes are predominantly that of simmonds type 1 and less of type 2 and 3 with the latter genotype currently not detected in indonesia. the 5' untranslated sequences are rela ... | 1995 | 7489348 |
| prevalence of antibodies to hepatitis c virus in relation to surrogate markers in a blood donor population of singapore. | prevalence of antibodies to hcv is studied among a blood donor population in singapore and its relationship to surrogate markers was examined. sequential serum samples from 4,091 blood donors were tested for the presence of anti-hcv using the second generation immunoassay (abbott). 275 random serum samples were tested for anti-hbc and alt. all the samples positive for anti-hcv were also tested for anti-hbc and alt. only 22 of the 4,091 donor samples (0.54%) were repeatedly reactive for anti-hcv. ... | 1993 | 7533946 |
| comparative results in detection of hcv antibodies by using a rapid hcv test, elisa and immunoblot. | viral hepatitis c is a major problem of post-transfusion hepatitis. the best measure for preventing hepatitis c infection in transfusion is blood donor screening. there are many methods for detecting hepatitis c virus antibodies. elisa is one of the sensitive screening methods. however it needs equipment, technical skills and it is time consuming. the genelabs diagnostics hcv-spot is a simple, rapid test for the detection of anti-hcv. this paper reports comparative results in detection of hcv an ... | 1994 | 7545315 |
| partial sequence analysis of the hepatitis c viral genome in singapore patients. | the distribution of hepatitis c viral (hcv) genotypes in singapore has not been previously determined. we studied the sera of 40 singapore patients which were pcr-positive for hcv. the hcv genotypes were determined by direct sequencing of amplified sequences of the 5' non-coding region, after reverse transcription. of the 40 samples, 35/40 (87.5%) were of hcv type 1, 2/40 (5.0%) were of type 2 and 3/40 (7.5%) were of type 3. the most common hcv genotype in this study was the type 1 genotype. our ... | 1994 | 8123037 |
| a study on the epidemiology of hepatitis c infection among blood donors in singapore. | hepatitis c viral (hcv) infection has been identified as the main cause of post-transfusion hepatitis (pth) since 1989. despite this, little is known regarding the prevalence and mode of transmission of the disease. the purpose of this study was therefore to study the demographic factors associated with hcv infection among singaporean blood donors. | 1995 | 8639336 |
| hepatitis c, e and g virus--three new viruses identified by molecular biology technique in the last decade. | the development of molecular biology techniques has enabled us to clone the genetic sequence of three new hepatitis viruses, namely, hepatitis c virus, hepatitis e virus and hepatitis g, or gbv-c virus in the last decade. hepatitis c virus (hcv) is now known to account for the majority of the parenterally transmitted non-a, non-b hepatitis and hepatitis e virus (hev) is the major cause of the epidemics of enterally transmitted non-a, non-b hepatitis in the less developed countries, mainly throug ... | 1997 | 9494678 |
| diversity of gb virus c/hepatitis g virus isolates in singapore: predominance of group 2a and the asian group 3 variant. | the occurrence of gbv-c/hgv infection was studied in 160 individuals from two high-risk groups in singapore. rt-pcr of the 5'-utr detected gbv-c/hgv rna in 3/73 (4.1%) of patients undergoing maintenance hemodialysis for chronic renal failure, and in 17/87 (19.5%) of patients coinfected with hcv who tested positive for hcv rna. phylogenetic analysis of 5'-utr sequences from these 20 samples showed that the asian or group 3 variant was found in 45% of the samples sequenced, thus confirming the hig ... | 1999 | 10335862 |
| identification and molecular characterisation of the complete genome of a singapore isolate of hepatitis c virus: sequence comparison with other strains and phylogenetic analysis. | the complete genomic sequence of a singapore isolate of the hepatitis c virus (hcv) was obtained from serum of an individual chronically infected with hcv. nine overlapping cdna clones covering the entire viral genome was amplified by reverse-transcription-polymerase chain reaction (rt-pcr), this isolate (hcv-s1) comprised 9,609 nucleotides (nt), including 341 nt of the complete 5' untranslated region (5' utr), a single open reading frame of 3,011 amino acids (aa) and 235 nt of the complete 3' u ... | 2001 | 11556407 |
| prevalence of hepatitis g virus infection in patients with liver diseases in singapore. | the prevalence of hepatitis g virus (hgv) infection in patients with liver diseases in singapore and its pathogenic role in these patients was studied. one hundred and forty-eight patients who had chronic hepatitis or acute non a-e hepatitis were studied. presence of hgv rna was determined by nested polymerase chain reaction of the 5'non-coding region of the virus in all the patients. hepatitis g igg antibody to the envelope (e2) antigen was tested with an enzyme immunoassay (boehringer mannheim ... | 2002 | 11993892 |
| treatment of chronic hepatitis c in patients with end-stage renal disease and hemophilia--the singapore experience. | the aim of this study was to determine the response to treatment with interferon-alpha (ifn-alpha) in patients with chronic hepatitis c who had end-stage renal disease (esrd) or hemophilia in singapore. | 2006 | 16166798 |
| femoral head banking: nuh tissue bank experience. | national university hospital tissue bank protocol follows guidelines recommended by the american association of tissue banks and the european association of tissue banks using donor selection criteria: medical history, clinical examination, chart review and laboratory tests for acquired immunodeficiency syndrome (aids), hepatitis b, hepatitis c, syphilis, and specimen for culture/sensitivity tests. for living donors, repeat testing is performed for aids and hepatitis c approximately 180 days aft ... | 2007 | 17424696 |
| seroprevalence of hepatitis c in intravenous opioid users presenting in the early phase of injecting drug use in singapore. | all over the world, hepatitis c virus (hcv) accounts for an estimated 130 million chronic infections. injection drug use has become one of the most important risk factors for hcv, and within the injection drug user population, the prevalence of hcv antibody ranges from 70 to 95 percent depending on an individual's length of use and the prevalence of infection in the community. this study was undertaken to determine the prevalence of and the risk factors for hepatitis c antibodies in injecting dr ... | 2007 | 17538746 |
| concomitant use of midazolam and buprenorphine and its implications among drug users in singapore. | the aim of this study was to determine the prevalence and characteristics of benzodiazepine (bzd) abuse among intravenous opioid users in singapore. | 2007 | 17925988 |
| risk factors and time-trends of cytomegalovirus (cmv), syphilis, toxoplasmosis and viral hepatitis infection and seroprevalence in human immunodeficiency virus (hiv) infected patients. | chronic bacterial, viral and parasitic infections contribute to the morbidity and mortality associated with human immunodeficiency virus (hiv) infection. this study investigated risk factors and time-trends of the seroprevalence of cytomegalovirus (cmv), toxoplasmosis and hepatitis a total antibody; and co-infection with syphilis, hepatitis b and hepatitis c among newly diagnosed hiv individuals in singapore. | 2013 | 24463828 |
| ten years of dengue drug discovery: progress and prospects. | to combat neglected diseases, the novartis institute of tropical diseases (nitd) was founded in 2002 through private-public funding from novartis and the singapore economic development board. one of nitd's missions is to develop antivirals for dengue virus (denv), the most prevalent mosquito-borne viral pathogen. neither vaccine nor antiviral is currently available for denv. here we review the progress in dengue drug discovery made at nitd as well as the major discoveries made by academia and ot ... | 2013 | 24076358 |
| dietary fatty acids and risk of hepatocellular carcinoma in the singapore chinese health study. | lipidomic signature of lipid metabolism suggests that omega-6 polyunsaturated fatty acids (pufa) may play a role in oncogenesis of hepatocellular carcinoma (hcc). hence, we examined the association between dietary fatty acids and risk of hcc. | 2016 | 26443688 |
| time for action on viral hepatitis. | the recent outbreak of hepatitis c virus (hcv) at singapore general hospital (sgh) has highlighted the dangers of viral hepatitis. in this case, infection control and environmental contamination were the culprits, particularly, a drop of blood containing 5 million iu hcv. from a broader perspective, there has been a revolution in hcv therapy with the recent rapid evolution of short-term (12 weeks) safe, all oral directly- acting antiviral (daa) therapy leading to cure rates of 90% to 100%, even ... | 2016 | 27118226 |
| targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for il28b genotyping. | a rapid, duplex, high-resolution melting interleukin-28b gene (il28b) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 dna samples from healthy volunteers. a linkage study on 300 healthy singaporeans showed variable haplotypes. when the assay was applied to plasma dna from 50 hepatitis c virus genotype-1 (hcv-1)-infected patients, five compound heterozygous types were detected. | 2012 | 22814458 |
| coffee, alcohol and other beverages in relation to cirrhosis mortality: the singapore chinese health study. | limited experimental and epidemiologic data suggest that coffee may reduce hepatic damage in chronic liver disease. the association between consumption of coffee and other beverages and risk of cirrhosis mortality was evaluated in the singapore chinese health study. this is a prospective population-based cohort of 63,275 middle-aged and older chinese subjects who provided data on diet, lifestyle, and medical histories through in-person interviews using a structured questionnaire at enrollment be ... | 2014 | 24753005 |
| cost-effectiveness of boceprevir co-administration versus pegylated interferon-α2b and ribavirin only for patients with hepatitis c genotype 1 in singapore. | patients infected with chronic hcv genotype 1 experience liver complications as the disease progresses. this study aims to project the long-term reduction of liver complications and cost-effectiveness of treatment strategies, including co-administrating boceprevir (boc) with pegylated interferon-α2b (peg-ifn) and ribavirin compared with standard of care (soc) of peg-ifn and ribavirin only. | 2015 | 25105844 |
| epidemiology and clinical evolution of liver cirrhosis in singapore. | liver cirrhosis is a common cause of morbidity and mortality and an important burden on the healthcare system. there is limited literature on liver cirrhosis in singapore. we aimed to describe the epidemiology and clinical characteristics of cirrhotic patients seen in an ambulatory setting in a tertiary referral centre. | 2015 | 26292950 |
| burden of blood transmitted infections in substance users admitted for inpatient treatment in singapore and the associated factors. | there is paucity of local data on the prevalence of blood transmitted infections (btis), such as hepatitis b, hepatitis c and human immunodeficiency virus (hiv) infections, among illicit drug users. this study aimed to examine the prevalence of btis among substance dependent inpatients and identify the factors associated with btis. | 2015 | 25588571 |
| simple novel bone bank storage: the singapore general hospital experience. | since 1981, the singapore general hospital (sgh) bone bank has proven to be a safe, reliable, and economical source of bone allografts. femoral heads are used exclusively and are procured from patients undergoing hip arthroplasty. screening for hiv, hepatitis b, and hepatitis c is carried out prior to surgery. patients with ongoing infection and past history of malignancy are excluded. the bone graft procured is washed and autoclaved to 134°c for 3 minutes. it is then stored in saline solution c ... | 2012 | 24845139 |
| a ten-year retrospective study on livedo vasculopathy in asian patients. | this study aims to analyse the clinico-epidemiological characteristics of asian patients diagnosed with livedo vasculopathy (lv). | 2012 | 23052434 |
| barcelona clinic liver cancer outperforms hong kong liver cancer staging of hepatocellular carcinoma in multiethnic asians: real-world perspective. | to compare the barcelona clinic liver cancer (bclc) and hong kong liver cancer (hklc) classification systems when applied to hcc patients from the largest tertiary-level centre in singapore. | 2017 | 28652658 |
| pretransplant model for end-stage liver disease score has no impact on posttransplant survival in living donor liver transplantation. | a high model for end-stage liver disease (meld) score≥25 has been reported to be associated with increased posttransplant mortality and morbidity among patients undergoing living donor liver transplantation (ldlt). we reviewed the results of patients undergoing ldlt at our transplant center for decompensated cirrhosis to determine whether a high meld impacted posttransplant survival. | 2012 | 22410026 |
| open-label phase 1b pilot study to assess the antiviral efficacy of simvastatin combined with sertraline in chronic hepatitis c patients. | 3-hydroxy-3-methyl-glutaryl coenzyme a reductase inhibitors inhibit hcv replication in vitro. the combination of sertraline and simvastatin has synergistic antiviral activity in vitro, but there are no prior in vivo studies. our aims were to prospectively assess the antiviral efficacy and safety of this drug combination in chronic hepatitis c (chc) patients. | 2011 | 22155916 |
| outcomes for kidney transplants at the national university health system: comparison with overseas transplants. | the 5-year and 10-year graft survivals for 186 deceased donor (dd) transplants performed at national university health system (nuhs) were 79.9% and 58.4% respectively. 5-year and 10-year patient survivals for dd transplants performed at nuhs were 94.2% and 83.4%. the 5-year and 10-year graft survivals for 128 living donor (ld) transplants performed at nuhs were 90.2% and 72% respectively. 5-year and 10-year patient survivals for dd transplants performed at nuhs were 98.6% and 95.1%. the projecte ... | 2010 | 21696038 |
| musculoskeletal tissue banking in singapore: 15 years of experience (1988-2003). | to report 15 years' experience of musculoskeletal tissue banking by the national university hospital tissue bank. | 2004 | 15621904 |
| seroprevalence of hepatitis b and c viral markers in patients with primary hepatocellular carcinoma in singapore. | the prevalence of hepatitis b and c serological markers were studied in 55 patients (45 males and 10 females) with primary hepatocellular carcinoma (phc). their ages ranged from 28 years to 79 years (mean age: 56 years). fifty-five other patients with non-hepatic diseases were used as age and sex matched controls. forty-one phc patients (74%) had chronic hepatitis b infection alone, 5 patients (9%) had chronic hepatitis c infection alone, 6 patients (11%) had chronic hepatitis b and c co-infecti ... | 1996 | 9046201 |
| hepatitis b surface antigen and antibody status in biopsy proven chronic hepatitis in singapore. | during the period between january 1975 and august 1976, 203 liver biopsies were received at the singapore general hospital from patients with a variety of liver diseases. a histological diagnosis of chronic hepatitis was made in 29 patients: 13 cases of chronic aggressive hepatitis (c.a.h.). 10 cases chronic persistent hepatitis (c.p.h.) and 6 of chronic lobular hepatitis (c.l.h.). c.p.h. and c.l.h. were found mainly in the third and fourth decades. c.a.h. was more common in the fifth to seventh ... | 1979 | 523186 |