Publications

occurrence of co-infection by leishmania (leishmania) chagasi and trypanosoma (trypanozoon) evansi in a dog in the state of mato grosso do sul, brazil.	a natural case of co-infection by leishmania and trypanosoma is reported in a dog (canis familiaris) in south- western state of mato grosso do sul, brazil. both amastigote and trypomastigote forms were observed after giemsa staining of cytological preparations of the dog's bone marrow aspirate. no parasite was detected using medium culture inoculation of the sample. dna obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (pcr) wi ...	2006	16410962
detection of natural infection in lutzomyia cruzi and lutzomyia forattinii (diptera: psychodidae: phlebotominae) by leishmania infantum chagasi in an endemic area of visceral leishmaniasis in brazil using a pcr multiplex assay.	in order to identify lutzomyia spp. naturally infected by leishmania parasites a pcr multiplex assay coupled to non-isotopic hybridization was used for the analysis of insect samples collected by cdc light traps in an endemic area of visceral leishmaniasis (vl) in the municipality of corumbá, mato grosso do sul state, brazil in may/june 2006. wild sand flies were identified and grouped into pools of 10 female specimens and 27 groups in total were collected. positive results were obtained from lu ...	2008	18502392
first report of natural infection of a bush dog (speothos venaticus) with leishmania (leishmania) chagasi in brazil.	we report here the first known case of natural infection of a bush dog with leishmania (leishmania) chagasi in brazil. the specimen was captured in the wild in the state of mato grosso and is currently being held in captivity at fundação jardim zoológico, rio de janeiro, brazil. the leishmaniasis was diagnosed by culture of promastigote forms in intact skin fragments and their characterization by isoenzyme electrophoresis. this report calls attention to the parasitological and etiological contro ...	2008	18036627
tesa-blot for the diagnosis of chagas disease in dogs from co-endemic regions for trypanosoma cruzi, trypanosoma evansi and leishmania chagasi.	we standardized serodiagnosis of dogs infected with trypanosoma cruzi using tesa (trypomastigote excreted-secreted antigen)-blot developed for human chagas disease. tesa-blot showed 100% sensitivity and specificity. in contrast, elisa using tesa (tesa-elisa) or epimastigotes (epi-elisa) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. when used in field studies in an endemic region for chagas disease, visceral leishmaniasis and trypanosoma evansi (mato grosso ...	2009	19426657
[leishmania (leishmania) infantum chagasi in wild canids kept in captivity in the state of mato grosso].	visceral leishmaniasis is a zoonosis that affects many mammals, and domestic canids are the main reservoirs in urban environments. this note describes infection by leishmania (leishmania) infantum chagasi among wild canids kept in captivity in the state of mato grosso, brazil.	2010	20563507
leishmania (leishmania) infantum/chagasi: histopathological aspects of the skin in naturally infected dogs in two endemic areas.	in the new world, visceral leishmaniasis (vl), which is a progressive disease and frequently fatal, is caused by leishmania (leishmania) infantum/chagasi. it is endemic in many regions of brazil and occasionally occurs in non-endemic regions when dogs from an endemic area are introduced. the aim of the present study is to compare different skin infection patterns of dogs from two leishmaniasis endemic areas. a histological analysis of dogs from campo grande, mato grosso do sul state, a region wh ...	2010	19854175
leishmania(leishmania) chagasi in captive wild felids in brazil.	this study used a pcr-rflp test to determine the presence of leishmania (leishmania) chagasi in 16 captive wild felids [seven puma concolor (linnaeus, 1771); five panthera onca (linnaeus, 1758) and four leopardus pardalis (linnaeus, 1758)] at the zoological park of the federal university of mato grosso, brazil. amplification of leishmania spp. dna was seen in samples from five pumas and one jaguar, and the species was characterized as l. chagasi using restriction enzymes. it is already known tha ...	2010	19740501
characterization of leishmania infantum species in dogs from the urban area of cuiabá, state of mato grosso, brazil.	visceral leishmaniasis presents urban behavior in some brazilian cities, with domestic dogs as the main infection source. in cuiabá, mt, canine visceral leishmaniasis was diagnosed and characterized as recommended by the ministry of health.	2011	22231252
[evidence of transmission of visceral leishmaniasis by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil].	the municipality of jaciara was classified in 2003 as a transmission area for visceral leishmaniasis in outbreak situations. this study aimed to establish evidence of transmission of leishmania (leishmania) infantum chagasi by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil.	2011	21340413
the genetic structure of leishmania infantum populations in brazil and its possible association with the transmission cycle of visceral leishmaniasis.	leishmania infantum is the etiologic agent of visceral leishmaniasis (vl) in the americas, mediterranean basin and west and central asia. although the geographic structure of l. infantum populations from the old world have been described, few studies have addressed the population structure of this parasite in the neotropical region. we employed 14 microsatellites to analyze the population structure of the l. infantum strains isolated from humans and dogs from most of the brazilian states endemic ...	2012	22606248
leishmania chagasi in opossums (didelphis albiventris) in an urban area endemic for visceral leishmaniasis, campo grande, mato grosso do sul, brazil.	we investigated the occurrence of leishmania infantum chagasi in didelphis albiventris opossums at a wild animal rehabilitation center in the city of campo grande, brazil. a total of 54 opossums were tested for l. i. chagasi infection in peripheral blood and bone marrow samples. the samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction-restriction fragment length polymorphism. leishmania i. chagasi dna was detected by polymerase chain reaction ...	2012	22802435
behavioral aspects of lutzomyia longipalpis (diptera: psychodidae) in urban area endemic for visceral leishmaniasis.	the study of some of the behavioral aspects of the main vector of leishmania infantum chagasi cunha & chagas in the americas, lutzomyia longipalpis (lutz & neiva), such as dispersion, population size, and vector survival rates, is important for the elucidation of the mechanisms of visceral leishmaniasis transmission. these parameters were studied by means of capture-mark-release-recapture experiments in an urban area of campo grande municipality, an endemic area of visceral leishmaniasis, situat ...	2013	23540114
spatial distribution and population genetics of leishmania infantum genotypes in são paulo state, brazil, employing multilocus microsatellite typing directly in dog infected tissues.	this study investigated the genetic characteristics of leishmania infantum samples from são paulo (sp) state, brazil in order to collaborate with information about the possible origins of the parasites, as well as, the introduction and spread of visceral leishmaniasis in this brazilian state. multilocus microsatellite typing (mlmt) was performed using a set of 17 microsatellite markers. dna was extracted from 250 samples collected from dogs diagnosed with visceral leishmaniasis and 112 (45%) wer ...	2013	23665466
spatial distribution and environmental factors associated to phlebotomine fauna in a border area of transmission of visceral leishmaniasis in mato grosso do sul, brazil.	mato grosso do sul has been undergoing a process of urbanization which results in loss of native vegetation. this withdrawal makes vectors of man and domestic animals closer, causing changes in the epidemiology of diseases such as american visceral leishmaniasis. the aim of the study was to evaluate the phlebotomine fauna and environmental issues related to the transmission of avl in ponta porã, mato grosso do sul, between 2009 and 2010.	2014	24898032
molecular and serological detection of tick-borne pathogens in dogs from an area endemic for leishmania infantum in mato grosso do sul, brazil.	tick-borne pathogens affect a wide range of vertebrate hosts. to identify tick-borne pathogens among dogs from campo grande, ms, brazil testing seropositive for leishmania infantum (syn. l. chagasi), a serological and molecular study was conducted to detect ehrlichia canis, anaplasma platys and babesia vogeli in 60 serum and spleen samples. a confirmatory diagnosis of l. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to ...	2014	24473877
sandflies in an urban area of transmission of visceral leishmaniasis in midwest brazil.	the phlebotomine fauna of campo grande city, capital of mato grosso do sul state in brazil, an endemic area for visceral leishmaniasis, has been thoroughly investigated, but all the insect collections were undertaken with automatic light traps. the present study sought to investigate the fauna in this city using shannon and disney traps, having human beings and hamsters, respectively, as bait. both types of traps were installed in forest fragment and peridomiciliary areas in the period from 2007 ...	2016	27593433
leishmania infantum as a causative agent of cutaneous leishmaniasis in the state of mato grosso do sul, brazil.	cutaneous leishmaniasis is caused by different species of the leishmania genus. leishmania(leishmania) infantum, causing cutaneous leishmaniasis, has been described in patients living in areas where visceral leishmaniasis is endemic. in this study, it was possible to characterize this species in seven slides from cutaneous tissue imprints from patients with cutaneous leishmaniasis in the state of mato grosso do sul, brazil.	2016	27007566
detection of leishmania spp. in bats from an area of brazil endemic for visceral leishmaniasis.	the multihost parasites leishmania spp. infect a broad range of wild mammalian species including bats. several species of bats have adapted to a variety of food resources and shelters in urban areas. this study aimed to detect leishmania spp. dna in bats present in forest fragments located in metropolitan areas endemic for leishmaniasis in campo grande, mato grosso do sul (ms), brazil. blood samples were obtained from 80 individuals, including eight species of phyllostomidae and one species of v ...	2017	28233434
serological detection of toxoplasma gondii, leishmania infantum and neospora caninum in cats from an area endemic for leishmaniasis in brazil.	an investigation was made into the occurrence of antibodies to toxoplasma gondii, leishmania infantum and neospora caninum in 151 domestic cats, based on the indirect fluorescent antibody test (ifat). serum samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats; 55 males and 96 females) in campo grande, mato grosso do sul, brazil between january and april 2013. igg antibodies to t. gondii, l. infantum and n. caninum were found, respectively, in 49 (32.5%), 34 (22.5% ...	2017	25517522
nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil.	recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ...	2017	28082638