pcr survey of vectorborne pathogens in dogs living in and around barcelona, an area endemic for leishmaniasis.blood samples from 153 dogs living in and around barcelona were assayed for leishmania infantum and ehrlichia, anaplasma, rickettsia, bartonella, hepatozoon, babesia and theileria species by pcr amplification of dna, and the amplicons obtained were sequenced. the prevalence of the infectious agents was l infantum (29.4 per cent), ehrlichia and anaplasma species (4.0 per cent), hepatozoon canis (3.3 per cent), babesia canis vogeli (2.0 per cent), babesia gibsoni (2.0 per cent), babesia canis cani ...200919168881
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
the life-cycle of leishmania infantum mon-77 in the priorat (catalonia, spain) involves humans, dogs and sandflies; also literature review of distribution and hosts of l. infantum zymodemes in the old world. 200111490994
epidemiology of canine leishmaniosis in catalonia (spain) the example of the priorat epidemiological survey of canine leishmaniosis was conducted in the priorat, a rural region in the northeast of spain, for 10 years (1985-1994). seroprevalence throughout the region, determined by dot-elisa and ifi, was 10.2% (8-12%). forty percent of the dogs studied had a low level of anti-leishmania antibodies, whereas only 50% were seronegative. only one-third of the seropositive dogs had evident symptoms of the disease. annual incidence of the disease was 5.7% and the level of endemicity ...199910392965
diagnostic potential of western blot analysis of sera from dogs with leishmaniasis in endemic areas and significance of the pattern.serum samples collected from 237 dogs in catalonia (northeastern spain) were screened by western blot analysis to detect the presence of antibodies specific to different leishmania infantum polypeptide fractions. leishmaniasis was confirmed in 72 of these dogs by direct examination and/or culture. another 165 animals from the priorat region were studied periodically for 2-8 years between 1987 and 1995, giving a total of 565 determinations. a control group of 93 dogs from nonendemic areas was als ...19989502597
leishmania infantum-specific igg, igg1 and igg2 antibody responses in healthy and ill dogs from endemic areas. evolution in the course of infection and after treatment.the expression of igg, igg1 and igg2 specific antibodies for leishmania infantum was studied in five groups of dogs in catalonia (spain): i, 99 asymptomatic dogs (infected and uninfected) from a highly endemic area for leishmaniosis; ii, 139 untreated dogs with clinically patent leishmaniosis; iii, 11 naturally infected asymptomatic dogs monitored for up to 5 years since they were found seropositive to leishmania antigen and without treatment; iv, 25 naturally infected dogs with clinically paten ...200111267753
vector-borne infections in cats: molecular study in barcelona area (spain).previous serological surveys have reported the presence of different organisms in cats from spain but little reports exist about the exact identity of these organisms. the purpose of the study reported here was to assess the presence of dna of several vector-borne infections in a population of cats from barcelona area. one hundred blood samples obtained from cats admitted to the uab-vth were entered into the study and classified as healthy (n=48) or unhealthy (n=52). edta-blood samples were assa ...200818079064
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