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evaluation of pcr assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods.the aims of this study are to identify leishmania species and compare and validate internal transcribed spacers (its) polymerase chain reaction (pcr) assay against parasitological methods for diagnosing cutaneous leishmaniasis (cl). we used the its-pcr, parasite culture, and microscopic evaluation of stained smears on 155 specimens from suspected cases of (cl) patients who referred to mashhad health centers (northeast iran). the pcr indicated the sensitivity (98.8%), correctly diagnosing 86 of t ...200818651180
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