sensitivity of test strategies used in the voluntary johne's disease herd status program for detection of mycobacterium paratuberculosis infection in dairy cattle evaluate sensitivities at the herd level of test strategies used in the voluntary johne's disease herd status program (vjdhsp) and alternative test strategies for detecting dairy cattle herds infected with mycobacterium paratuberculosis.200212420786
a retrospective study of mortality in pennsylvania captive white-tailed deer (odocoileus virginianus): 20000--2003.the postmortem records of 160 white-tailed deer (odocoileus virginianus) submitted for necropsy examination from 59 separate pennsylvania captive deer farms over a 3.5-year period were reviewed to determine the primary cause of death of each animal. the most common causes of death were bronchopneumonia (39 cases), enterocolitis (30 cases), malnutrition (13 cases), and trauma (11 cases). other causes of mortality included severe gastrointestinal parasitism (6 cases), cellulitis with septicemia (5 ...200415586566
fecal shedding of mycobacterium avium subsp. paratuberculosis by dairy cows.between 1982 and 2000, fecal samples were obtained from 786 cows that were shedding mycobacterium avium subsp. paratuberculosis (map). these cows were resident on 93 pennsylvania dairies (mean herd size, 64 milk cows) that had no or minimal previous testing for map. feces were cultured on four tubes of herrold's egg yolk medium and the distribution of mean map colony forming units (cfu) was evaluated. most cows were light (< 10 cfu/tube, 51.4%) or high (> 50 cfu/tube, 30.8%) fecal shedders with ...200515863285
transmission parameters of mycobacterium avium subspecies paratuberculosis infections in a dairy herd going through a control program.a johne's disease control program, including stringent management practices and a test-and-cull program (whole-herd fecal-samples taken twice a year), was implemented on a medium-sized pennsylvania dairy farm that was suffering losses from clinical johne's disease. the data that emerged from the control program, combined with birthdates, culling dates, lactation information and pedigrees, yielded an extensive longitudinal dataset. the dataset was processed through sas 9.1 for statistical analysi ...200817868937
a whole genome association analysis identifies loci associated with mycobacterium avium subsp. paratuberculosis infection status in us holstein cattle.the purpose of this study was to identify loci associated with mycobacterium avium subspecies paratuberculosis (map) infection status in us holsteins using the illumina bovinesnp50 beadchip whole genome single nucleotide polymorphism (snp) assay. two hundred forty-five cows from dairies in new york, pennsylvania and vermont enrolled in longitudinal herd studies between january 1999 and november 2007 were assessed for the presence of map in both faecal and tissue samples. an animal was considered ...200919422364
correlation between herrold egg yolk medium culture and real-time quantitative polymerase chain reaction results for mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental samples.real-time quantitative polymerase chain reaction (qpcr) testing for mycobacterium avium subspecies paratuberculosis (map) in fecal samples is a rapid alternative to culture on herrold egg yolk medium (heym), the traditional antemortem reference test for map. although the sensitivity and specificity of these 2 tests have been estimated based on dichotomized test results, the correlation between real-time qpcr threshold cycle (ct) values and colony-forming units (cfu) on heym for fresh and thawed ...201020807920
environmental contamination with mycobacterium avium subsp. paratuberculosis in endemically infected dairy herds.environmental contamination with mycobacterium avium subsp. paratuberculosis (map) is thought to be one of the primary sources of infection for dairy cattle. the exact link between fecal shedding of map by individual cows and environmental contamination levels at the herd level was explored with a cross-sectional analysis of longitudinally collected samples on 3 dairy farms. composite samples from multiple environmental sites in 3 commercial dairy herds in the northeast us were cultured quarterl ...201121775002
producer survey of bird-livestock interactions in commercial dairies.the objective of this producer survey was to identify and estimate damage caused by bird-livestock interactions in commercial dairies. the interactions between birds and livestock have previously been implicated in causing economic damage while contributing to the environmental dissemination of microorganisms pathogenic to livestock and humans. very little research exists to help producers understand what bird species use dairies, why they use dairies, or the scope and nature of damage created a ...201222981584
evaluation of performance of bacterial culture of feces and serum elisa across stages of johne's disease in cattle using a bayesian latent class model.the objective of this study was to evaluate the performance of bacterial culture of feces and serum elisa to correctly identify cows with mycobacterium avium ssp. paratuberculosis (map) at heavy, light, and non-fecal-shedding levels. a total of 29,785 parallel test results from bacterial culture of feces and serum elisa were collected from 17 dairy herds in minnesota, pennsylvania, and colorado. samples were obtained from adult cows from dairy herds enrolled for up to 10 yr in the national johne ...201526364104
notes from the field: mycobacterium chimaera contamination of heater-cooler devices used in cardiac surgery - united the spring of 2015, investigators in switzerland reported a cluster of six patients with invasive infection with mycobacterium chimaera, a species of nontuberculous mycobacterium ubiquitous in soil and water. the infected patients had undergone open-heart surgery that used contaminated heater-cooler devices during extracorporeal circulation (1). in july 2015, a pennsylvania hospital also identified a cluster of invasive nontuberculous mycobacterial infections among open-heart surgery patients ...201627740609
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