herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in wisconsin.a random sample of wisconsin dairy herds, stratified by herd size, were tested for paratuberculosis by use of an absorbed elisa procedure. the elisa was optimized for overall accuracy by means of receiver operating characteristic curve analysis, and had a sensitivity and specificity of 50.9 and 94.9%, respectively. herd prevalence was analyzed for correlation with responses to a management practices questionnaire completed by the herd owners. one hundred and fifty-eight herds and 4,990 cattle we ...19948163422
sensitivity of test strategies used in the voluntary johne's disease herd status program for detection of mycobacterium paratuberculosis infection in dairy cattle evaluate sensitivities at the herd level of test strategies used in the voluntary johne's disease herd status program (vjdhsp) and alternative test strategies for detecting dairy cattle herds infected with mycobacterium paratuberculosis.200212420786
detection of viable mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and pcr.cattle with johne's disease can shed live mycobacterium avium subsp. paratuberculosis (map) in their milk, and map can survive under simulated commercial pasteurization conditions. in several studies conducted in the united kingdom and canada, map dna has been detected in retail pasteurized milk samples; however, in one study in the united kingdom viable map was identified in commercially pasteurized milk. a double-blind study involving two laboratories was undertaken to evaluate retail pasteuri ...200515895728
detection of mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in wisconsin and minnesota by pcr.research has been focused on the detection of mycobacterium avium subspecies paratuberculosis (map) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. therefore, map contamination in milk-derived products must be investigated. we undertook a six-month study to investigate the presence of viable map and map genetic components in cheese curds purchased from retail outlets in the northern and southern regions of wisconsin and minnesota. a total of 98 r ...200616545540
mycobacterium avium subsp. paratuberculosis in scavenging mammals in wisconsin.the presence of mycobacterium avium subsp. paratuberculosis (map) in non-ruminant wildlife has raised questions regarding the role of these species in johne's disease transmission. in this study we tested 472 tissues from 212 animals of six different species of scavenging mammals. all animals were taken from within a 210-square-mile area in dane and iowa counties of south central wisconsin from september to may in 2003-04 and tested for the presence of map. we detected map-specific dna in 81 of ...200717495318
efficacy of feeding plasma-derived commercial colostrum replacer for the prevention of transmission of mycobacterium avium subsp paratuberculosis in holstein estimate the relative risk of paratuberculosis (johne's disease [jd]) in calves fed a plasma-derived colostrum-replacement (cr) product versus raw bovine maternal colostrum (mc).200919405889
Evaluation of the risk of paratuberculosis in adult cows fed Mycobacterium avium subsp paratuberculosis DNA-positive or -negative colostrum as calves.To estimate the risk of subclinical Mycobacterium avium subsp paratuberculosis (MAP) infection in cows that ingested MAP DNA-positive raw colostrum as calves, compared with risk in cows that ingested MAP DNA-negative raw colostrum as calves.201122023123
producer survey of bird-livestock interactions in commercial dairies.the objective of this producer survey was to identify and estimate damage caused by bird-livestock interactions in commercial dairies. the interactions between birds and livestock have previously been implicated in causing economic damage while contributing to the environmental dissemination of microorganisms pathogenic to livestock and humans. very little research exists to help producers understand what bird species use dairies, why they use dairies, or the scope and nature of damage created a ...201222981584
epizootic of paratuberculosis in farmed elk.after multiple cases of chronic diarrhea and weight loss in a farmed elk herd, 3 yearlings and 1 adult elk with similar clinical signs were euthanatized and necropsied. gross and histologic evidence of paratuberculosis were found in the yearlings. evidence of serum antibody to mycobacterium paratuberculosis was detected in the 2 elk with the most disseminated infection. acid-fast organisms were isolated from multiple organs in all 4 elk and identified as m paratuberculosis by dna probe. thirty-f ...19989810391
evidence of birth seasonality and clustering of mycobacterium avium subspecies paratuberculosis infection in us dairy herds.paratuberculosis (johne's disease) is a contagious intestinal infection of ruminants caused by mycobacterium avium subspecies paratuberculosis (map). in cattle, young calves are at the highest risk for acquiring the infection which occurs mainly through ingestion of map from contaminated milk, colostrum and feces or environmental contacts. data consisted of birth dates and elisa results of 8000 mature cows from 24 jersey herds from throughout the us and 4 wisconsin holstein herds. some herds als ...201323973011
implications of pcr and elisa results on the routes of bulk-tank contamination with mycobacterium avium ssp. paratuberculosis.mycobacterium avium ssp. paratuberculosis (map), the etiologic agent of johne's disease in dairy cattle, may enter the bulk tank via environmental contamination or direct excretion into milk. traditionally, diagnostics to identify map in milk target either map antibodies (by elisa) or the organism itself (by culture or pcr). high elisa titers may be directly associated with excretion of map into milk but only indirectly linked to environmental contamination of the bulk tank. patterns of bulk-mil ...201626686723
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