mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of mato grosso do sul, brazil.the presence of mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of mato grosso do sul, brazil. seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by ziehl-neelsen staining. polymerase chain reaction (pcr) using primers specific for m. bovis identified m. bovis in 13 (76.5%) isolat ...200616410964
evaluation of the efficiency of nested q-pcr in the detection of mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of mato grosso, brazil.bovine tuberculosis (btb) is a zoonotic disease caused by mycobacterium bovis, a member of the mycobacterium tuberculosis complex (mtc). the quick and specific detection of this species is of extreme importance, since btb may cause economic impacts, in addition to presenting imminent risks to human health. in the present study a nested real-time pcr test (nested q-pcr) was used in post-mortem evaluations to assess cattle carcasses with btb-suspected lesions. a total of 41,193 cattle slaughtered ...201525863190
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