| dengue serologic survey in ribeirão preto, são paulo, brazil. | the city of ribeirão preto suffered a dengue 1 epidemic that began in november 1990 and ended in march 1991. a serologic survey designed to detect igg antibodies to the four dengue serotypes and other flaviviruses was carried out in ribeirão preto during september and october of 1992. dengue 1 antibodies were detected in 5.4% of the survey participants. significantly higher seropositivity (9.3%) was found among subjects residing in the northwest sector of ribeirão preto than among those living i ... | 1995 | 7757124 |
| [dengue and its vectors in brazil]. | history of dengue in brazil is covered from the first citations in the xixth century to the great outbreaks of the last ten years. den-1 and den-4 viruses have been isolated for the first time in 1982 during an epidemic in boa vista, roraima state. in 1986-1987, epidemics of dengue type 1 covered an extended area from rio de janeiro/sào paulo states to the north east states of brazil. during 1990-1991, dengue type 2 epidemics have been notified in the south east (rio de janeiro/são paulo) and in ... | 1996 | 8924771 |
| factors favoring houseplant container infestation with aedes aegypti larvae in marília, são paulo, brazil. | since reinvasion of são paulo state by the aedes aegypti (l.) mosquito in 1985, flower pots and vases have been important larval habitats despite educational messages focusing on their control. the objectives of this study were to characterize flower pots and vases as larval habitats with respect to the quantities present and infested, the types of plants involved, and the specific locations of the mosquito larvae; to explore local names for houseplants; and to examine factors affecting acceptan ... | 1997 | 9149524 |
| [epidemiology of dengue infection in ribeirão preto, sp, brazil]. | to assess dengue transmission in a correctional facility for juvenile delinquents in ribeirão preto, state of são paulo, brazil. | 2002 | 12045796 |
| susceptibility of urban and rural populations of aedes albopictus from são paulo state, brazil, to infection by dengue-1 and -2 viruses. | in brazil, dengue viruses (denv) are transmitted by aedes aegypti (l.). however, the recent introduction of aedes albopictus (skuse) has caused concern, because some populations of this species also are capable of denv transmission. ae. albopictus from rural and urban localities of são paulo state, brazil, were artificially exposed to infection with denv-1 and -2 to evaluate their susceptibility. the rates of infection (6.2 and 5.7% for denv-1; 5.3 and 12% for denv-2) and dissemination (22.2 and ... | 2004 | 15535628 |
| application of an alternative aedes species (diptera: culicidae) surveillance method in botucatu city, sao paulo, brazil. | one of the main problems with dengue is the control of aedes aegypti, its major vector. in brazil, the current control program for ae. aegypti and aedes albopictus populations includes larval density surveys. an interesting alternative is the use of a distinct index, the premise condition index (pci). this tool relates conditions of property, such as houses and yards, and the degree of shade with the occurrence of aedes sp. oviposition, and is calculated as scores from 3 to 9. the lowest score i ... | 2005 | 16103596 |
| complete genome characterization of rocio virus (flavivirus: flaviviridae), a brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in sao paulo state. | the flaviviruses of major medical importance in south american countries are yellow fever, dengue, saint louis encephalitis, west nile and rocio viruses. rocio virus (rocv) has been responsible for epidemics of severe encephalitis in brazil with a case-fatality rate of 10 % and development of sequelae in 20 % of the survivors. we have sequenced and characterized the entire genome of rocv for the first time, by determining the general traits of the open reading frame and the characteristics of vi ... | 2007 | 17622628 |
| spatio-temporal tracking and phylodynamics of an urban dengue 3 outbreak in são paulo, brazil. | the dengue virus has a single-stranded positive-sense rna genome of approximately 10.700 nucleotides with a single open reading frame that encodes three structural (c, prm, and e) and seven nonstructural (ns1, ns2a, ns2b, ns3, ns4a, ns4b, and ns5) proteins. it possesses four antigenically distinct serotypes (denv 1-4). many phylogenetic studies address particularities of the different serotypes using convenience samples that are not conducive to a spatio-temporal analysis in a single urban setti ... | 2009 | 19478848 |
| detection of dengue virus in saliva and urine by real time rt-pcr. | early diagnosis of dengue virus (denv) infection is important for patient management and control of dengue outbreaks. the objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of denv infection by real time rt-pcr. two febrile patients, who have been attended at the general hospital of the school of medicine of ribeirao preto, sao paulo university were included in the study. serum, urine and saliva samples collected from both patients were subjecte ... | 2010 | 20105295 |
| [analysis on the spatial-temporal distribution of dengue and larval infestation in the municipality of tupã, state of são paulo]. | the dengue virus is transmitted by the bite of the mosquito aedes aegypti and the current control program has not met the target of preventing its transmission. this study aimed to analyze the relationship between the spatial-temporal distribution of dengue cases and the larval indices in the municipality of tupã between january 2004 and december 2007. | 2010 | 20464143 |
| characterization of dengue virus type 2: new insights on the 2010 brazilian epidemic. | dengue viruses (denv) serotypes 1, 2, and 3 have been causing yearly outbreaks in brazil. in this study, we report the re-introduction of denv2 in the coast of são paulo state. partial envelope viral genes were sequenced from eighteen patients with dengue fever during the 2010 epidemic. phylogenetic analysis showed this strain belongs to the american/asian genotype and was closely related to the virus that circulated in rio de janeiro in 2007 and 2008. the phylogeny also showed no clustering by ... | 2010 | 20676363 |
| spatial distribution of the risk of dengue fever in southeast brazil, 2006-2007. | abstract: background: many factors have been associated with circulation of the dengue fever virus and vector, although the dynamics of transmission are not yet fully understood. the aim of this work is to estimate the spatial distribution of the risk of dengue fever in an area of continuous dengue occurrence. methods: this is a spatial population-based case-control study that analyzed 538 cases and 727 controls in one district of the municipality of campinas, sao paulo, brazil, from 2006-2007, ... | 2011 | 21599980 |
| etiologies of rash and fever illnesses in campinas, brazil. | few population-based studies of infectious etiologies of fever-rash illnesses have been conducted. this study reports on enhanced febrile-rash illness surveillance in campinas, brazil, a setting of low measles and rubella virus transmission. | 2011 | 21954258 |
| dengue virus type 4 phylogenetics in brazil 2011: looking beyond the veil. | dengue fever and dengue hemorrhagic fever are diseases affecting approximately 100 million people/year and are a major concern in developing countries. in the present study, the phylogenetic relationship of six strains of the first autochthonous cases of denv-4 infection occurred in sao paulo state, parana state and rio grande do sul state, brazil, 2011 were studied. nucleotide sequences of the envelope gene were determined and compared with sequences representative of the genotypes i, ii, iii a ... | 2011 | 22216365 |
| microevolution of aedes aegypti. | scientific research into the epidemiology of dengue frequently focuses on the microevolution and dispersion of the mosquito aedes aegypti. one of the world's largest urban agglomerations infested by ae. aegypti is the brazilian megalopolis of sao paulo, where >26,900 cases of dengue were reported until june 2015. unfortunately, the dynamics of the genetic variability of ae. aegypti in the sao paulo area have not been well studied. to reduce this knowledge gap, we assessed the morphogenetic varia ... | 2015 | 26360876 |
| finding aedes aegypti in a natural breeding site in an urban zone, sao paulo, southeastern brazil. | this is the description of how nine aedes aegypti larvae were found in a natural breeding site in the pinheiros neighborhood, city of sao paulo, sp, southeastern brazil. the record was conducted in december 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. findingae. aegypti larvae in a tree hole shows this species' ability to use both artificial and natural environments as breeding sites a ... | 2016 | 26982959 |
| são paulo urban heat islands have a higher incidence of dengue than other urban areas. | urban heat islands are characterized by high land surface temperature, low humidity, and poor vegetation, and considered to favor the transmission of the mosquito-borne dengue fever that is transmitted by the aedes aegypti mosquito. we analyzed the recorded dengue incidence in sao paulo city, brazil, in 2010-2011, in terms of multiple environmental and socioeconomic variables. geographical information systems, thermal remote sensing images, and census data were used to classify city areas accord ... | 2015 | 25523076 |
| aedes aegypti entomological indices in an endemic area for dengue in sao paulo state, brazil. | to evaluate the most productive types of properties and containers for aedes aegypti and the spatial distribution of entomological indices. | 2013 | 24346572 |
| cost of production of live attenuated dengue vaccines: a case study of the instituto butantan, sao paulo, brazil. | a vaccine to prevent dengue disease is urgently needed. fortunately, a few tetravalent candidate vaccines are in the later stages of development and show promise. but, if the cost of these candidates is too high, their beneficial potential will not be realized. the price of a vaccine is one of the most important factors affecting its ultimate application in developing countries. in recent years, new vaccines such as those for human papilloma virus and pneumococcal disease (conjugate vaccine) hav ... | 2012 | 22406455 |
| estimating potential demand and supply of dengue vaccine in brazil. | dengue is endemic in brazil. several dengue vaccine candidates, including one at the butantan institute in sao paulo, are being evaluated in clinical trials and may be licensed in several years. this study estimates the potential doses of dengue vaccine needed in brazil under different scenarios in the first 5 years after vaccine introduction. estimates were based on 2015-2022 country population projections. an estimated country population of 200-209 million with an annual 3.3-3.5 million cohort ... | 2011 | 21734468 |
| phylogenetic characterization of dengue virus type 2 in espírito santo, brazil. | over the last decade, the espírito santo state, brazil has become an endemic dengue fever location with annual outbreaks of varying magnitude. it is still unclear which geographical route allowed the virus entry in the state and how it has genetically changed since then. therefore we have set out to study the local molecular constitution of the virus and determine phylogenetic similarities and differences with other brazilian locations, as well as locations worldwide. viral envelope genes were p ... | 2011 | 21562769 |
| [dengue: sero-epidemiological survey and virological surveillance in campinas, são paulo, brazil]. | the epidemiology of dengue in the municipality of campinas, são paulo, brazil, was studied in 1998 using a randomized sero-epidemiological survey. epidemiological surveillance data from 1996-2003 were also analyzed, with an emphasis on virological surveillance. 1,260 individuals participated in the survey and had blood samples drawn by finger stick on filter paper. blood samples were tested by eia-icc, an enzyme immunoassay using infected cells as antigen. dengue antibody prevalence (14.79%) was ... | 2007 | 17334580 |
| molecular analysis of the dengue virus type 1 and 2 in brazil based on sequences of the genomic envelope-nonstructural protein 1 junction region. | the genomic sequences of the envelope-non-structural protein 1 junction region (e/ns1) of 84 den-1 and 22 den-2 isolates from brazil were determined. most of these strains were isolated in the period from 1995 to 2001 in endemic and regions of recent dengue transmission in são paulo state. sequence data for den-1 and den-2 utilized in phylogenetic and split decomposition analyses also include sequences deposited in genbank from different regions of brazil and of the world. phylogenetic analyses ... | 2004 | 15286818 |
| [dengue fever: a post-epidemic sero-epidemiological survey in an urban area setting at a northwestern county of são paulo state, brazil]. | the objective of this study was to evaluate the real size of the epidemics registered in the urban area of the county of santa bárbara d'oeste, sp, brazil, from april to june, 1995. the measurement of the epidemiological validity of the official surveillance system criteria and its positive predicted value were adopted as specific goals. | 1999 | 10689373 |
| [laboratory diagnosis and symptoms of dengue, during an outbreak in the ribeirão preto region, sp, brazil]. | a dengue type 1 outbreak started in the ribeirao preto region, north of sao paulo state, brazil, in november of 1990. about 3500 dengue cases were confirmed by blood tests until february of 1991. the virus research unit of the faculty of medicine of ribeirao preto-sao paulo state university, studied 502 dengue suspect cases. the serologic diagnosis of dengue type 1 was confirmed by haemagglutination inhibition test (hai) in 19% of the cases. diagnosis was done later by using an enzyme immuno ass ... | 1992 | 1340025 |