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standardisation of an indirect enzyme-linked immunosorbent assay for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis) in italy.an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis linnaeus, 1758). the test accuracy was evaluated on milk samples from the campania region in italy. a total of 100 negative samples were collected from 10 officially brucellosis-free herds in salerno province, while 30 positive samples were collected from 3 herds in caserta province, where animals gave positive results to the official tests and ...201121240852
efficacy of brucella abortus vaccine strain rb51 compared to the reference vaccine brucella abortus strain 19 in water buffalo.approximately 250,000 water buffalo (bubalus bubalis) live in the campania region of southern italy where the breeding of this species is very popular. of these animals, almost 150,000 are concentrated in the caserta province where the prevalence of brucella abortus in this species represents approximately 20% at herd level. the italian brucellosis eradication programme provides a slaughter and vaccination strategy for this province. b. abortus strain rb51 (rb51) has become the official vaccine ...201020391363
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