| bacillus tequilensis sp. nov., isolated from a 2000-year-old mexican shaft-tomb, is closely related to bacillus subtilis. | a gram-positive, spore-forming bacillus was isolated from a sample taken from an approximately 2000-year-old shaft-tomb located in the mexican state of jalisco, near the city of tequila. tentative identification using conventional biochemical analysis consistently identified the isolate as bacillus subtilis. dna isolated from the tomb isolate, strain 10b(t), and closely related species was used to amplify a bacillus-specific portion of the highly conserved 16s rrna gene and an internal region of ... | 2006 | 16825615 |