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modification of christensen urease test as an inexpensive tool for detection of helicobacter pylori.about half the world population is infected with helicobacter pylori. most live in developing countries where clinical studies face the constraints of high costs of imported rapid diagnostic tests. in this work, we describe and validate a simple local urease test (lut) to determine the presence of the bacterium in gastric biopsies, and report the incidence of infection among symptomatic patients in caracas, venezuela. statistical comparison of lut and clotest (delta west, bentley, australia) (n ...19979294705
short report: socioeconomic and seasonal variations of helicobacter pylori infection in patients in venezuela.infection by helicobacter pylori is recognized as a risk factor for gastric cancer and peptic ulcer disease. venezuela has regions with different gastric cancer risks; the andean region has the highest gastric cancer mortality in the country. we performed a cross-sectional study on 357 patients who underwent endoscopy attending 2 private (n = 76) and one public hospital in caracas, venezuela (n = 215), and one public hospital in the andes (n = 66) to determine h. pylori infection (by a rapid bio ...200212135267
detection of helicobacter pylori dna in the oral cavity and gastroduodenal system of a venezuelan population.dental plaque has been suggested as a reservoir for helicobacter pylori but the hypothesis that the oral microflora may be a permanent reservoir of h. pylori is still controversial. the aims of this study were to determine the presence of h. pylori dna in the gastric antrum and dental plaque of a venezuelan population by pcr and to investigate the relationship between this infection and the oral hygiene index. thirty-two patients from the hospital universitario de caracas, attending for routine ...200212358067
east asian genotypes of helicobacter pylori strains in amerindians provide evidence for its ancient human carriage.phylogenies of indigenous microbes have been used as surrogates for the origins of the hosts that carry them. conversely, polymorphisms may be used to date the spread of a microbial species when information about their host populations is available. therefore, we examined polymorphisms in helicobacter pylori, which persistently colonize the human stomach, to test the hypothesis that they have been ancient inhabitants of humans. three h. pylori loci that previously have been shown to have phyloge ...200212417749
high frequency of gastric colonization with multiple helicobacter pylori strains in venezuelan subjects.multiple helicobacter pylori strains may colonize an individual host. using enzyme-linked immunosorbent assay and line probe assay (lipa) techniques, we analyzed the prevalence of mixed h. pylori colonization in 127 subjects from venezuela, a country of high h. pylori prevalence, from three regions representing different population groups: the andes (merida), where caucasian mestizos predominate, a major city near the coast (caracas), where amerindian-caucasian-african mestizos predominate, and ...200515956377
[importance of igg anti-caga antibodies of helicobacter pylori in venezuelan patients with gastric diseases].helicobacter pylori is one of the most common bacterial infections worldwide. it is associated with chronic gastritis, peptic ulcer disease and constitutes a major risk factor for gastric adenocarcinoma and lymphoma. the aim of this study was to evaluate the specific serologic immunoglobulin g (igg) response to whole cells proteins, caga and urease antigens of helicobacter pylori in a venezuelan population. we evaluated 66 patients from the hospital universitario de caracas, attending in the gas ...200516353543
helicobacter pylori infection in rural and urban dyspeptic patients from venezuela.the goal of this work was to assess the helicobacter pylori prevalence in a rural mestizo population and compare it to an urban population from venezuela. the study was performed in gastric juice samples of 71 dyspeptic patients from caracas (urban) and 39 from tucupita (rural), in the orinoco delta region. helicobacter pylori was detected by amplification of 16s rrna, glmm, and urea genes in 55.0% patients from urban and 87.2% from rural populations. caga was found positive in 51% and 62% urban ...201526195456
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