| differential diagnosis of goatpox virus in taiwan by multiplex polymerase chain reaction assay and high-resolution melt analysis. | the a32l gene from a goatpox virus (gtpv) strain isolated from a goat in yunlin county (taiwan) displays several substitutions compared with the sequence of the kenyan gtpv vaccine strain sgp0240 and the pellor gtpv strain. samples from the skin lesions on 6 goats with gtpv infection or from goats with orf virus (orfv) infection were tested in a multiplex polymerase chain reaction (pcr) system that used primers gpf, gpr1, and gpr2 as well as previously published primers specific for orfv. these ... | 2014 | 24567543 |
| purification and functional motifs of the recombinant atpase of orf virus. | our previous study showed that the recombinant atpase encoded by the a32l gene of orf virus displayed atp hydrolysis activity as predicted from its amino acids sequence. this viral atpase contains four known functional motifs (motifs i-iv) and a novel aydg motif; they are essential for atp hydrolysis reaction by binding atp and magnesium ions. the motifs i and ii correspond with the walker a and b motifs of the typical atpase, respectively. to examine the biochemical roles of these five conserve ... | 2011 | 21540113 |
| phylogenetic analysis and characterization of korean orf virus from dairy goats: case report. | an outbreak of orf virus infection in dairy goats in korea was investigated. suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. orf virus was detected by electron microscopy and viral dna was identified by pcr. to reveal the genetic characteristics of the korean strain (orf/09/korea), the sequences of the major envelope protein (b2l) and orf virus interferon resistance (vir) genes were determined and then compared with published referenc ... | 2009 | 19835585 |
| differential diagnosis of orf viruses by a single-step pcr. | the complete nucleotide sequence of the a32l gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an atpase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central taiwan. dna sequence analysis of the a32l genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. this finding led to the de ... | 2009 | 19409929 |
| phylogenetic analysis of parapoxviruses and the c-terminal heterogeneity of viral atpase proteins. | two outbreaks of orf virus (a parapoxvirus) infection in goats found in nantou and taiping of central taiwan were investigated. the nucleotide and the amino acid sequences of viral b2l, e3l and a32l genes in these two outbreaks were analyzed, and each of their phylogenetic trees were also constructed. in the a32l gene, an unexpected deletion of 24 nucleotides was found in the taiping strain. the a32l gene can encode an atpase and is supposed to be involved in virion dna packaging. the 24 nucleot ... | 2009 | 19061942 |
| identification and phylogenetic analysis of orf virus from goats in taiwan. | an outbreak of contagious ecthyma in goats in central taiwan was investigated. the disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (pcr) and sequence of major envelope protein (b2l) gene. the entire protein-coding region of b2l gene were cloned and sequenced. phylogenetic analysis of b2l amino acid sequences showed that the orf virus identified in this outbreak was ... | 2007 | 17682935 |
| suppression of influenza virus infection by the orf virus isolated in taiwan. | orf virus (orfv), a member of parapoxvirus, is an enveloped virus with genome of double-stranded dna. orfv causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. in general, detection of viral dna and observing orfv virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the orfv in cell culture using virus-containing tissue as inoculum is known to be difficult. in this work, the orfv (hoping st ... | 2015 | 25855509 |