typing of human group a rotavirus with alkaline phosphatase-labeled oligonucleotide probes or a monoclonal enzyme immunoassay in unfrozen stools of children with diarrhea in developed countries, serotypes (or g types) have been identified in > 70% of group a rotavirus using monoclonal enzyme immunoassays (meias); however, these assays have identified < 50% of rotavirus g types from developing countries presumably because the vp7 antigens were damaged by freezing and thawing during transportation of specimens. the vp7 (g) serotypes of rotavirus in unfrozen stool collected from children with acute diarrhea in bangkok were determined using meia and compared to hybri ...19957714487
typing of human group a rotavirus with alkaline phosphatase-labeled oligonucleotide probes.rotavirus (rv) in stools of children less than 1 year of age with diarrhea in bangkok in 1989 were serotyped by monoclonal enzyme immunoassay (meia). rna extracted from these specimens was tested for hybridization with alkaline phosphatase (ap) and 32p-labeled oligonucleotides constructed from the nucleotide sequences of vp7 of human g types 1 (hug1ac), 2 (hug2ac), 3 (hug3ac), and 4 (hug4ac). of 148 specimens that contained rv, 72% (106/148) hybridized with rv g type specific ap-labeled oligonuc ...19921331307
the incidence of rotavirus a isolates of g genotype in thailand in 2002-2004.rotaviruses are the leading cause of severe gastroenteritis among infants and young children worldwide. between november 2002 and march 2004, 36 stool specimens of 108 children with acute diarrhea in bangkok, thailand were found positive for rotavirus a (rv-a) by rt-pcr. the 36 isolates were subjected to genotyping by rflp analysis and direct sequencing of a part of the gene for major outer capsid glycoprotein vp7. the sequences obtained were subjected to phylogenetic analysis. among the isolate ...200516047738
molecular characterization of group a human rotaviruses in bangkok and buriram, thailand during 2004-2006 reveals the predominance of g1p[8], g9p[8] and a rare g3p[19] strain.the aim of this study has been to determine the incidence of diverse human rotavirus strains circulating in thailand between october 2004 and april 2006 by means of molecular characterization. pediatric patients aged between 2 months and 5 years diagnosed with acute diarrhea (n=307) in bangkok and buriram, thailand were tested for human rotavirus a (rv-a) by rt-pcr. a total of 130 specimens (42.3%) were found rv-a positive and 126 were characterized by direct sequencing of the capsid glycoprotei ...200818228123
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