| bovine piroplasms in minorca (balearic islands, spain): a comparison of pcr-based and light microscopy detection. | the present study provides the first epidemiological data regarding infection by theileria and babesia piroplasms in cattle in minorca. more than 94% of the studied animals were positive for the presence of theileria sp., and of those, 41.3% were positive for the presence of theileria annulata. these results indicate that the prevalence of mediterranean theileriosis caused by t. annulata is very high in minorcan dairy farms and that other theileria sp. are also present in the area. the prevalenc ... | 2001 | 11502372 |
| reverse line blot hybridization used to identify hemoprotozoa in minorcan cattle. | piroplasmosis, a tick-borne protozoal disease, is an important disease affecting domestic and wild animals. we performed pcr-based reverse line blot hybridization (rlb) assays on blood samples obtained from 133 cattle exposed to ticks in field conditions in minorca (balearic islands, spain) in three different seasons. the oligonucleotides used were those for theileria annulata, t. buffeli, t. taurotragi, t. velifera, babesia bigemina, b. bovis, b. divergens, and b. major. the rlb technique allow ... | 2002 | 12381567 |
| development and evaluation of a real-time pcr assay for the quantitative detection of theileria annulata in cattle. | the tick-borne apicomplexan bovine parasite theileria annulata is endemic in many tropical and temperate areas, including minorca (balearic islands, spain). real-time pcr is widely used for the detection of piroplasms but quantification is not commonly considered. | 2012 | 22889141 |
| monitoring piroplasms infection in three cattle farms in minorca (balearic islands, spain) with previous history of clinical piroplamosis. | bovine piroplasmosis are tick-borne protozoan diseases caused by parasites of the genera theileria and babesia. three friesian cattle farms (f1-f3) with previous history of clinical piroplamosis were selected in minorca (balearic islands, spain). blood samples were collected from 8 to 11 animals every two months throughout a year and, a newly developed multiplex dna bead-based suspension array based on the luminex(®) xmap technology was used to monitor for the presence of piroplasms. the assay i ... | 2012 | 22884914 |