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canine filariosis in umbria: an update of the occurrence one year after the first observation of autochthonous foci.following the first observation of two autochthonous foci of canine filariosis occurred in umbria region in the year 2001, a survey on prevalence and risk factors was conducted 12 months later to better understand the actual entity of the dirofilaria problem in umbria region. blood samples were collected between january and december 2002 from 2406 dogs living in a total of 7 towns located in the identified areas at risk. blood samples were tested by a modified knott's technique to evaluate the m ...200315267001
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