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retrospective serosurvey for human granulocytic ehrlichiosis agent in urban white-footed mice from maryland.archived serum samples from 111 peromyscus leucopus, collected 1984-88 in baltimore city (maryland, usa), were analyzed by indirect immunofluorescence assay. sera from two (2%) individuals contained antibodies reactive to ehrlichia equi and the human granulocytic ehrlichiosis (hge) agent. this suggests that the hge agent or an antigenically related organism has been present in rodent populations in this locality for more than a decade, and was present in the region at least 12 yr before a case o ...19989476244
serologic evidence of infection with ehrlichia spp. in wild rodents (muridae: sigmodontinae) in the united states.rodent (muridae: sigmodontinae) blood and sera collected from 14 states were tested for seroreactivity to a cultured isolate of the human granulocytic ehrlichiosis (hge) agent by using an indirect immunofluorescence assay. of the 1,240 samples tested, 136 (11%) were found to be reactive at titers of > or = 32. rodents with hge agent-specific antibodies were found in new york (23% of 491 samples; geometric mean endpoint titer [gmt] = 441), connecticut (11% of 100 samples; gmt = 481), california ( ...19989508298
antibodies to granulocytic ehrlichiae in white-footed and cotton mice in eastern united states.serum samples, collected from peromyscus leucopus (white-footed mouse) or peromyscus gossypinus (cotton mouse) during 1987 through 1990 in florida, georgia, maryland, mississippi, and north carolina (usa), and in 1997 in southern connecticut were analyzed by indirect fluorescent antibody (ifa) staining methods or western blot procedures for antibodies to granulocytic ehrlichiae. of the 82 sera from white-footed mice in connecticut tested by ifa methods with either the bds or nch-1 strain of the ...199910231752
serologic evidence for rickettsia typhi and an ehrlichial agent in norway rats from baltimore, maryland, usa.we screened serum from 90 norway rats trapped in east baltimore, maryland, usa, from april to november 2005 for antibodies against rickettsia typhi and ehrlichia chaffeensis. six rats had positive titers of > or = 1:64 against r. typhi and did not react with r. akari. in addition, four rats had cross-reactive antibodies with titers of > or = 1:64 against ehrlichia chaffeensis. sera from these rats also cross-reacted with anaplasma phagocytophilum or ehrlichia muris. our data indicate that the ag ...200616989563
serosurvey for selected pathogens in free-ranging american black bears (ursus americanus) in maryland, usa.american black bears (ursus americanus) in maryland, usa, live in forested areas in close proximity to humans and their domestic pets. from 1999 to 2011, we collected 84 serum samples from 63 black bears (18 males; 45 females) in five maryland counties and tested them for exposure to infectious, including zoonotic, pathogens. a large portion of the bears had antibody to canine distemper virus and toxoplasma gondii, many at high titers. prevalences of antibodies to zoonotic agents such as rabies ...201425075540
co-circulating microorganisms in questing ixodes scapularis nymphs in maryland.ixodes scapularis can be infected with borrelia burgdorferi, anaplasma phagocytophilum, bartonella spp., babesia microti, and rickettsia spp., including spotted-fever group rickettsia. as all of these microorganisms have been reported in maryland, the potential for these ticks to have concurrent infections exists in this region. to assess the frequency of these complex infections, 348 i. scapularis nymphs collected in 2003 were screened for these microorganisms by pcr with positives being confir ...200718260514
prevalence of bartonella species, haemoplasma species, ehrlichia species, anaplasma phagocytophilum, and neorickettsia risticii dna in the blood of cats and their fleas in the united states.ctenocephalides felis were killed and collected from 92 cats in alabama, maryland, and texas. the fleas and blood from the corresponding cat were digested and assessed in polymerase chain reaction assays that amplify dna of ehrlichia species, anaplasma phagocytophilum, neorickettsia risticii, mycoplasma haemofelis, 'candidatus m haemominutum' and bartonella species. dna consistent with b henselae, b clarridgeiae, m haemofelis, or 'candidatus m haemominutum' was commonly amplified from cats (60.9 ...200616290092
serologic evidence of a natural infection of white-tailed deer with the agent of human granulocytic ehrlichiosis in wisconsin and maryland.white-tailed deer participate in the maintenance of the ixodes tick life cycle and are reservoirs for some tick-borne infectious agents. deer may be useful sentinels for tick-transmitted agents, such as ehrlichiae. in order to determine whether white-tailed deer are markers of natural transmission or are reservoirs for the human granulocytic ehrlichiosis (hge) agent, we performed indirect immunofluorescent-antibody (ifa) tests and immunoblotting with the hge agent and ehrlichia chaffeensis on se ...19989801331
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