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application of local products r-phycoerythrin and monoclonal antibody as a fluorescent antibody probe to detect entamoeba histolytica trophozoites.r-phycoerythrin (r-pe) was extracted from red algae, gracilaria fisheri from pattani province, thailand, with 50 mm sodium phosphate buffer, ph 7.0, followed by precipitation with 30-50% final concentration of saturated ammonium sulphate solution at 0 degree c. the precipitate was further purified by deae-cellulose (de-52) column chromatography. the purified r-pe showed a single band of mr 240 kda by polyacrylamide gel electrophoresis with the maximum absorption and maximum fluorescence emission ...19969279993
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