investigation of the prevalence of amoebiasis in izmir province and determination of entamoeba spp. using pcr and enzyme immunoassay.amoebiasis is a common and life-threatening disease. the discrimination of the pathogenic entamoeba histolytica from the non-pathogenic entamoeba dispar could be done by advanced methods such as enzyme immunoassay (eia) and pcr. the aim of this study was to investigate the prevalence of amoebiasis in izmir province, and differentiate the entamoeba species by pcr and eia. stool samples of 2,047 individuals were examined by direct microscopy, formalin ethyl acetate concentration, trichrome stainin ...200818843895
an outbreak of gastroenteritis associated with intestinal parasites.intestinal protozoa are one of the leading causes of waterborne outbreaks. stool samples of 196 residents from a village of izmir, using the public water supply, were collected during an outbreak of gastroenteritis. patients were asked to fill out a questionnaire reporting on gender, age, gastrointestinal symptoms, whether or not there was a toilet in the house, their hygiene practices, and similar symptoms in the household members. of the patients who had gastrointestinal symptoms (74.5%), diar ...200818985582
differentiation of entamoeba histolytica and entamoeba dispar by pcr: a preliminary study in izmir, turkey.the causative agent of amoebiasis is currently attributed to two distinct species (e. histolytica and e. dispar). the aim of this study was to differentiate these species by pcr in stool samples. isolated genomic dna was amplified by pcr and band products of 101 bp (e. dispar) were obtained. all seven stool samples were found to be e. dispar, not e. histolytica. our results demonstrated the significance of e. histolytica/dispar differentiation in the diagnosis of amoebiasis. this study is prelim ...200717319599
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