genetic and phenotypic characterization of borrelia burgdorferi strains isolated from ixodes ricinus ticks in the province of bolzano, italy.lyme disease is caused by three borrelial species, b. burgdorferi sensu stricto, b. garinii and borrelia group vs461. in a restricted biotope of the bolzano province, in the caldaro community, five clones of two borrelial variants were isolated from ixodes ricinus ticks. a preliminary serological study showed that the two variants cross-reacted with b. burgdorferi b31 and b. garinii n34 strains, respectively. the isolates were genomically related with strains b31 and n34, respectively, sharing a ...19957603344
antigenic and genomic analysis of a borrelia burgdorferi sensu stricto strain isolated from ixodes ricinus ticks in alto adige-south tyrol, italy.a borrelia burgdorferi sensu lato strain isolated from ixodes ricinus ticks in alto adige-south tyrol (northern italy) was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole cell proteins, western immunoblotting analysis (wba) with polyclonal and monoclonal antibodies, and pulsed-field gel electrophoresis (pfge). the isolate named bz6 was identified as belonging to the genospecies b. burgdorferi sensu stricto on the basis of its protein profile and its reac ...19989744686
human seroprevalence against borrelia burgdorferi sensu lato in two comparable regions of the eastern alps is not correlated to vector infection rates.seroprevalences were determined by testing sera of 1607 blood donors from north, east, and south tyrol. in the tyrols, the continental divide delimitates areas with high seroprevalences of igg antibodies against borrelia burgdorferi sensu lato in the north (7.2%) from areas with low seroprevalences in the south (1.5%). to determine borrelia prevalences in unfed ixodes ricinus ticks, 755 questing ticks were tested by pcr. prevalences in nymphal and adult ticks were found to be 19.7% (n=132) and 2 ...201525661649
genospecies of borrelia burgdorferi sensu lato in ixodes ricinus ticks from the autonomous province of trento, italy.sequences of the variable intergenic spacer region 5s (rrfa) 23s (rrlb) rrna were used to identify borrelia genospecies present in ixodes ricinus nymphs collected from the lamar lakes area of the province of trento, italy (overall prevalence=6.3%). four genospecies were identified, one for the first time in this province (b. valaisiana), and three which have been noted previously (b. afzelii, b. garinii, and b. burgdorferi s.s.). in order to compare the genetic variability of these genospecies i ...200717137840
prevalence of borrelia burgdorferi s.l. and anaplasma phagocytophilum in the wood tick ixodes ricinus in the province of trento, italy. 200617033789
seroprevalence study of tick-borne encephalitis, borrelia burgdorferi, dengue and toscana virus in turin province.tick borne encephalitis virus (tbev) is present in some european countries and it is transmitted by a tick bite. ixodes ricinus is the main vector of the infection in italy, where fortunately clinical neurological manifestations, typical of the more serious phase of the disease, are very rarely observed. this behaviour is different from other endemic euroasiatic areas where numerous cases of encephalitis are described. however tbe transmission has not been widely investigated in italy and availa ...200716312014
[ixodes ricinus, transmitted diseases and reservoirs].the tick ixodes ricinus has been recorded in most italian regions especially in thermo-mesophilous woods and shrubby habitats where the relative humidity allow the tick to complete its 3 year developmental cycle, as predicted for the european climatic ranges. this tick acts both as vector and reservoir for a series of wildlife zoonotic pathogens, especially the agents of lyme diseases, tick borne encephalitis and human granulocytic ehrlichiosis, which are emerging in most of europe. to assess th ...200415305699
geographical information systems and bootstrap aggregation (bagging) of tree-based classifiers for lyme disease risk prediction in trentino, italian alps.the risk of exposure to lyme disease in the province of trento, italian alps, was predicted through the analysis of the distribution of ixodes ricinus (l.) nymphs infected with borrelia burgdorferi s.l. with a model based on bootstrap aggregation (bagging) of tree-based classifiers within a geographical information system (gis). data on l ricinus density assessed by dragging the vegetation in 438 sites during 1996 were cross-correlated with the digital cartography of a gis, which included the va ...200212061445
lyme disease in italy, 1983-1996.this paper is a brief review of the epidemiology of lyme disease in italy. the first case of the illness was identified by crovato in liguria in 1983. in the following years, many other cases have been reported from all italian regions with the exception of valle d'aosta, basilicata and calabria. the exact number of cases in our country is not known because lyme disease was not a notifiable disease until 1990, but on the basis of literature data, at least 1324 cases have been observed in the fou ...19989812324
ixodes ricinus (acari: ixodidae) infestation on roe deer (capreolus capreolus) in trentino, italian alps.the most important tick-deer system potentially supporting the epidemiology of lyme disease in the italian alps is that regarding ixodes ricinus (l.) and roe deer (capreolus capreolus l.). in this study, the pattern of tick infestation on 562 male roe deer harvested in september 1994 in 56 game districts of trentino, northern italy, was assessed. the prevalence and density of infestation by i. ricinus were analyzed by a model based on classification and regression trees (cart), using both discre ...19979419849
classification tree methods for analysis of mesoscale distribution of ixodes ricinus (acari:ixodidae) in trentino, italian alps.cases of lyme disease and tick-borne encephalitis were recognized recently in the province of trento, italian alps. assessment of areas of potential risk for these tick-borne diseases is carried out by a model based on classification and regression trees (cart), using both discrete and continuous variables. data on ixodes ricinus (l.) occurrence resulted from extensive sampling carried out by standard methods in 99 sites over an area of approximately 2,700 km2 in the province of trento. a series ...19968961635
antibodies to borrelia burgdorferi in sheep and goats. alto adige-south tyrol, italy.a serologic survey for antibodies to borrelia burgdorferi was conducted on sheep and goat serum samples collected in alto adige-south tyrol, italy, in 1990. sera were tested by indirect immune fluorescence assay (iifa) and microbial adherence immobilization assay (maia). iifa and/or maia anti-b. burgdorferi antibodies were detected in 14.1% of the 269 sheep and 36.8% of the 133 goats examined. iifa and maia were both positive in 4 out of 38 positive sheep sera (10.5%) and 21 out of 49 positive g ...19968722314
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