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positive findings of borrelia burgdorferi in culex (culex) pipiens pipiens larvae in the surrounding of brno city determined by the pcr method.after first finding borrelia in the midgut of imago mosquitoes, we concentrated on the presence of borrelia in mosquito development stages--larvae of the third or fourth instar. in the summer season in the years of 2000-2001 a total of 439 culex (culex) pipiens pipiens larvae were collected from a barrel of rainwater in the obrany holiday area of brno city (east moravia, czech republic). the larvae midgut was observed under dark-field microscopy. ten dfm positive samples (2.28%) were further ana ...200212498597
isolation, cultivation and identification of borrelia burgdorferi genospecies from ixodes ricinus ticks from the city of brno, czech republic.a total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected by flagging of vegetation in suburban woods of pisarky park (city of brno) from july to october 2002. the midgut of each tick was dissected out and transferred individually into bsk-h medium. after cultivation, all specimens were examined by dark-field microscopy (dfm) for the presence of borreliae. out of 305 tick samples, 45 were (14.8%) dfm positive. the following polymerase chain reaction (pcr) then revealed ...200717655181
serologic survey of the wild boar (sus scrofa) for borrelia burgdorferi sensu lato.sera of 642 wild boars (sus scrofa) shot by hunters in ten administrative regions of the czech republic during 1995-2000, were tested by indirect hemagglutination assay (iha) for the presence of anti-borrelia igg. antibodies to borrelia burgdorferi sensu lato (bb) were detected in serum samples from all 10 regions, and overall seroprevalence rate was 12.8%. titres of antibodies ranged from 1:80 to 1:640. borrelia antibodies were most frequent in the animals from three administrative regions of t ...200918973452
a longitudinal study of the prevalence of borreliae in ticks in the urban locality of brno-pisárky, czech republic. 200819263859
[monitoring the prevalence of pathogenic borrelia burgdorferi sensu lato in ticks in two brno locations.]objective: the aim of the article is to present the results of monitoring the prevalence of the spirochete borrelia burgdorferi sensu lato (bbsl) in ixodes ricinus ticks in two different locations in the city of brno, pisárky and zamilovaný hájek (zh). at the same time, factors potentially affecting the activity of ticks in these locations were studied. results of the monitoring carried out in the first three spring months of the year 2011 are compared with 2010 results. material and methods: ...201122161755
identification of borrelia burgdorferi genospecies isolated from ixodes ricinus ticks in the south moravian region of the czech republic.during 2008-2012, a total of 466 ticks ixodes ricinus removed from humans were collected and tested for the presence of borrelia burgdorferi sensu lato (bbsl). ticks were collected in all districts of the south moravian region of the czech republic (cz).201526706968
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