molecular evidence for anaplasma phagocytophilum and borrelia burgdorferi sensu lato in ixodes ricinus ticks from eastern slovakia.ixodes ricinus ticks (20 males, 20 females and 20 nymphs) collected in kosice, slovakia were examined for the presence of anaplasma phagocytophilum and borrelia burgdorferi sensu lato (s.l.) by pcr. 38.3 % of the tested ticks carried single infection of b. burgdorferi s.l. and 8.3 % were infected with a. phagocytophilum. double infection of both pathogens was detected in 5 % of tested ticks. these results indicate that both b. burgdorferi s.l. and a. phagocytophilum co-circulate in the enzootic ...200314677924
[igg antibodies to borrelia in dogs in the area of kosice].an elisa investigation showed that 26.9% of dogs in kosice region were positive for anti-borrelia igg antibodies. a significantly higher seroprevalence was detected only in hunting dogs (40.0%) compared with service dogs (11.8%; p < 0.05). an analysis of seroprevalence by age showed a significant difference only between dogs in the categories 1-3 years (37.8%) and under 1 year of age (8.3%; p < 0.05). in other age groups no significant differences in seroprevalence were observed. no significant ...19968686135
The genospecies B. burgdorferi s.l., isolated from ticks and from neurological patients with suspected Lyme borreliosis.Lyme borreliosis (LB) is the most disseminated tick-borne disease in the Northern hemisphere, and infestation with ticks is one of the essential factors influencing transmission of the disease to humans. This work intends to compare the occurrence of borrelia circulating in indigenous ticks and in patients suffering from neurological diseases.201121876490
occurrence of igg antibodies to anaplasma phagocytophilum in humans suspected of lyme borreliosis in eastern slovakia.human granulocytic anaplasmosis (hga) and lyme borreliosis (lb) are tick-borne and emerging infectious diseases caused by the anaplasma phagocytophilum and borrelia burgdorferi species. in europe, including slovakia, the principal vector of both pathogens is the common tick - ixodes ricinus, in which double infections with these pathogens have been reported. the aim of our study was evidence of igg antibodies against a. phagocytophilum in blood sera of humans with suspects lb from several clinic ...200920047263
elisa comparison of three whole-cell antigens of borrelia burgdorferi sensu lato in serological study of dogs from area of kosice, eastern slovakia.three antigens of borrelia burgdorferi sensu lato (b. burgdorferi sensu stricto--slovak strain ir 105, b. garini - slovak strain k 24 and b. burgdorferi sensu stricto--american strain b 31) were compared by elisa on a group of dogs from urban agglomeration of kosice, eastern slovakia. of 256 serum samples from dogs examined for the presence of anti-borrelia igg antibodies, 128 (50%) were positive with ir 105 antigen, 107 (41.7%) with k 24 and 74 (28.9%) with b 31. the seroprevalence between stra ...19989852559
potential foci of lyme borreliosis in 1997 the presence of ticks ixodes ricinus infected with the agent of lyme borreliosis was detected on the vegetation of kosice urban agglomeration. the density of ticks on the vegetation of pericentral area islands of forest stands and neglected gardens was 2.4 times lower than that observed in a peripheral park forest. only sporadical findings of ticks were reported from the industrial central part of town with remnants of uncultivated vegetation. central parks and housing estates were actua ...200010965444
occurrence of borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii in the ixodes ricinus ticks from eastern slovakia.a total of 2816 unfed adults nymphs of ixodes ricinus ticks were collected from vegetation in kosice (eastern slovakia) from 1994 to 1997. prevalence of borrelia burgdorferi s. l. in i. ricinus ticks, detected by dark field microscopy, varied and depended upon the year and the habitat of the collected ticks. the lowest prevalence was observed in 1994 (4.8%). during 1995 it increased to 17.2% and during the next two years decreased to 15.5% and 14.2%. the rate of infection varied from 2.1 to 23.3 ...200010845258
[preliminary identification of borrelia burgdorferi isolated from ixodes ricinus ticks in eastern slovakia].the first finding of spirochetes borrelia burgdorferi sensu lato in eastern slovakia pertains to ticks ixodes ricinus collected in the kosice park forest in september 1995. the authors tested 7 strains using immunoblotting with 4 monoclonal antibodies (mab): h9724--specific to the genus borrelia, identifying 41 kda flagellar protein, h5332--specific to ospa protein of b. burgdorferi, j 8.3 and i 17.3 specific to ospa and ospb proteins of b. afzelii. all the strains belong to the genus borrelia ( ...19979162456
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