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prevalence of borrelia burgdorferi s.i. in ixodes ricinus ticks from styria (austria) and species identification by pcr-rflp analysis.a total of 1163 i. ricinus ticks were collected in 3 different regions (15 localities) in styria (austria) in june 1997 and examined for the presence of spirochetes by dark field microscopy. the mean infection rate was 20.8%. among 310 adults, 24.2% were positive and among 853 nymphs, 19.6% were positive. all 15 collection areas were shown to harbour infected nymphs with a positivity rate ranging from 5.8% (3/52) to 32.1% (18/56). isolation attempts in bskii medium resulted in 29 isolates. speci ...19989987185
prevalence of borrelia burgdorferi sensu lato in the tick ixodes ricinus in the styrian mountains of austria.a total of 691 ixodes ricinus (22 male, 39 female, 501 nymphs and 129 larvae), the tick vector of lyme borreliosis, were collected by flagging from vegetation in 11 areas at altitudes between 789 m and 1350 m above sea level in mixed woodland with pasture land (cattle) in the province of styria in austria. the ticks were individually examined for presence of borrelia burgdorferi sensu lato by dark-field microscopy and 107 of them by real-time pcr. attempts to cultivate borreliae were made in bsk ...200617160607
horses and borrelia: immunoblot patterns with five borrelia burgdorferi sensu lato strains and sera from horses of various stud farms in austria and from the spanish riding school in vienna.grazing animals are continuously exposed to tick bites. consequently, one may expect that horses will become infected with the various pathogens carried by ticks including borrelia burgdorferi sensu lato. whether horses may develop clinical disease due to this pathogen is controversially discussed. we were interested to learn about the infection of horses with borrelia burgdorferi sensu lato within one season by studying the dynamics of the humoral immune response in paired blood samples. the ma ...200212141766
[seroepidemiological studies of zoonotic infections in hunters in southeastern austria--prevalences, risk factors, and preventive methods].the aim of this study was to investigate the seroprevalences to zoonotic pathogens in hunters, to propose preventive measures and to obtain more information about the occurrence of zoonotic pathogens in local wild animal populations. from 146 male and 3 female hunters originating from the south-eastern austrian federal states of styria and burgenland blood samples were taken and anamnestic data were obtained using a questionnaire. the serological investigations included the following viral, bact ...200312894685
the outcome of lyme borreliosis in children.austria is an endemic area for lyme borreliosis. the igg seroprevalence of healthy blood donors as investigated by a dako flagellum-elisa in graz/styria is 13%. in order to determine whether this high seroprevalence is caused by infection in childhood, 36 children aged 3 to 18 years (mean, 10.1 years) were followed up over 2 to 20 months (mean, 11.1 months) and reinvestigated for clinical symptoms and antibodies against b. burgdorferi by a commercial flagellum elisa and a commercial b. garinii w ...199910666805
prevalence of antibodies to borrelia burgdorferi flagellin in styrian blood donors.lyme borreliosis and tick-borne encephalitis (tbe) are the most common diseases in austria caused by tick bites. tbe endemic areas are well defined. it seemed to be of interest to compare prevalence data of antibodies against borrelia burgdorferi (b.b.) to tbe endemic and non endemic areas. blood samples (n = 1162) were obtained from healthy blood donors in combination with a standardized questionnaire during 21 excursions to 7 selected regions of styria, austria. serum samples were screened for ...19938219495
[sero-epidemiological studies of zoonotic infections in hunters--comparative analysis with veterinarians, farmers, and abattoir workers].the aim of this study was to investigate seroprevalences to zoonotic pathogens in hunters, to compare the results with other predisposed occupational groups already investigated and to propose preventive measures. blood samples were taken from 146 male and 3 female hunters from the provinces of styria and burgenland in the south-east of austria and anamnestic data were obtained using a questionnaire. the serological investigations included the following bacterial, viral and parasitic zoonotic ag ...200315508783
species identification of borrelia burgdorferi sensu lato from tick and human isolates in styria (austria) by pcr-rflp analysis.seventy-one isolates of borrelia burgdorferi sensu lato (b.b.s.l.) derived from ixodes ricinus ticks (50 strains) and patients (21 strains) were characterised by pcr-rlfp analysis. in four cases the human isolates were obtained from the cerebrospinal fluid (csf) of patients with clinical symptoms of neuroborreliosis and in 17 cases from skin biopsies of patients with dermatological manifestation of lyme borreliosis. ixodes ricinus isolates originated from 14 localities in three regions (mur vall ...199910666817
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