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serodiagnosis of borreliosis: indirect immunofluorescence assay, enzyme-linked immunosorbent assay and immunoblotting.lyme disease is an infectious, multi-system, tick-borne disease caused by genospecies of borrelia burgdorferi bacteria sensu lato, characterized by remarkable heterogeneity. in this situation choosing an optimal antigen array for diagnostic tests seems problematic. the serological tests for borrelia routinely done in laboratories often produce ambiguous results, which makes a proper diagnosis rather complicated and thus delays the implementation of an appropriate treatment regimen. thirty-seven ...201121258869
prevalence of dna and antibodies to borrelia burgdorferi sensu lato in dogs suspected of borreliosis.the aim of the paper was an attempt to correlate clinical signs with the presence of dna of borrelia burgdorferi (sensu lato) s.l. and the antibodies against b. burgdorferi s.l. in the blood of dogs. among the animals studied there were 62 dogs delivered to the veterinary clinic in szczecin and 30 from the municipal animal shelter in szczecin with varied clinical signs of borreliosis. in all cases the owners admitted frequent contacts of their dogs with ticks, both in the past, as well as shortl ...200516457474
[seasonal activity of the ticks ixodes ricinus in the lakesides and forests habitats of szczecin and environs and its infestation with the spirochete borrelia burgdorferi].the article presents the results of researche (1999-2000) on numbers and seasonal activity of ixodes ricinus in selected lakesides and forests habitats of szczecin and on occurence of the spirochetes borrelia burgdorferi sensu lato in ticks. a total of 8065 ticks were collected from 6 localities : 647 larvae, 4549 nymphs, 1624 females and 1244 males. the spirochetes b. burgdorferi were found (using the pcr techniques) in 2271 specimens e.c. 28.1% whole population. numbers infected larvae were 21 ...200116894752
detection of borrelia burgdorferi sensu lato in mosquitoes (culicidae) in recreational areas of the city of szczecin.the mosquitoes were caught within the period of their highest activity, i.e. june august 2001, in recreational areas of the city of szczecin. spirochetes, borrelia burgdorferi sensu lato were detected in mosquitoes with the aid of the method of indirect immunofluorescence (ifa) using rabbit anti-borrelia burgdorferi antibodies and goat anti-rabbit igg marked with fluorescein isothiocyanate (fitc). a total of 639 mosquito females representing genera aëdes (99.1%) and culex (0.9%) were collected. ...200212088398
the prevalence of spirochete borrelia burgdorferi sensu lato in ticks ixodes ricinus and mosquitoes aedes spp. within a selected recreational area in the city of szczecin.the aim of this study was to determine the prevalence of spirochete borrelia burgdorferi s.l. in ticks ixodes ricinus and mosquitoes aedes spp. within the bukowa forest, collected between 2000 and 2001. the study covered 215 ticks (193 nymphs and 22 adults) and 947 mosquitoes female of the genus aedes. spirochetes of borrelia burgdorferi s.l. were detected in the arthropods studied with the method of indirect immunofluorescence assay (ifa). positive readings of the immunological reaction were st ...200415236506
coexistence dna of borrelia burgdorferi sensu lato and babesia microti in ixodes ricinus ticks from north-western poland.the tick ixodes ricinus may carry microorganisms which cause serious human and animal diseases, i.a., the lyme disease (borreliosis), caused by the spirochaete borrelia burgdorferi sensu lato and babesiosis, induced by the protozoan babesia microti. both microbe species may co-occur in the same and other species of the genus tick and produce a mixed infection in humans and animals. the major objective of the study was to identify dna of b. burgdorferi and b. microti in the i. ricinus ticks colle ...200212088393
[isolation of borrelia burgdorferi sensu lato in ticks ixodes ricinus by polymerase chain reaction (pcr)].attempts were made to identify the causative orgamsm of lyme disease in szczecin from tick ixodes ricinus as a vector. ticks were collected in 1997 year in forest areas of szczecin, from localites associated with numerous attendance of people. the method used in this study was the polymerase chain reaction (pcr) on the flagellin structural gene fla of borrelia burgdorferi sensu stricto. the flagellin pcr primer set reaction was conservative for b. burgdorferi sensu stricto, b. afzelii and b. gar ...200016886357
[anaplasma phagocytophila and protozoans of babesia genus in dogs from endemic areas of lyme disease in north-western poland].infections caused by the spirochete borrelia burgdorferi sensu lato may be accompanied by other microorganisms, such as anaplasma, ehrlichia and babesia. these pathogens are transmitted by the ticks and are a risk to humans and animals. ixodes ricinus ticks collected from recreational areas of szczecin and northwestern poland contained dna of the pathogens mentioned above and cases of double and triple coinfection have been documented. the aim of this paper was to determine if dogs suspect to ti ...200416865968
[studies on lyme disease incidence rates in selected groups of forestry workers in west pomerania, 2005-2014].the data collected by sanitary-epidemiological stations in 2005-2014 were analyzed to determine the incidence rates of borreliosis lyme disease in the west pomerania group of workers exposed to tick bites.201728345681
the occurrence of borrelia burgdorferi sensu lato in the populations of ixodes ricinus in forest areas of szczecin during 2000-2001.the aim of the present study was to determine the seasonal abundance of ticks, ixodes ricinus and their infection frequency with spirochetes, borrelia burgdorferi in wooded areas of the vicinity of the city of szczecin during 2000-2001. the ticks were collected from april to october. presence of borrelia burgdorferi sensu lato was detected with the method of indirect immunofluorescence (ifa). the results showed differences in seasonal activity of ticks within the years of the study. in the year ...200312852727
ticks and mosquitoes as vectors of borrelia burgdorferi s. l. in the forested areas of szczecin.the aim of the study was to determine the infection level of adult forms and larvae of ticks and mosquitoes with borrelia burgdorferi in the forested areas of szczecin. a total of 1699 ticks ixodes ricinus, including 1422 nymphs, 277 adult forms and 2862 mosquito females representing the genera aedes (89.6%) and culex (10.4%) were collected between the years 2004 and 2005. a further 3746 larvae and 1596 pupae of culex pipiens pipiens were colleted from water bodies. borrelia burgdorferi s. l. wa ...200718274258
identification of borrelia burgdorferi genospecies inducing lyme disease in dogs from western poland.canine lyme borreliosis may be caused by three borrelia burgdorferi sensu lato genospecies. the prevalence of infection by borrelia species was determined by nested polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) with the enzyme fsp4h i in the blood of dogs naturally infested by ticks in an endemic region of poland. blood samples were collected from 98 dogs of various breeds, delivered to the veterinary clinic in szczecin (northwestern poland) for various reas ...200515782655
[epidemiologic-clinical aspects of tick borne borreliosis in the szczecin province].the aim of the study was to assess if szczecin voivodeship is an area of endemic borreliosis, assess the risk due to b. burgdorferi infection in habitants and evaluate clinical manifestations of borreliosis. the study was conducted in 1993-1995, material comprised 299 persons (211 men and 88 women) aged from 3 to 78 years, divided into two groups. group i consisted of foresters working in four forestry districts, occupationally exposed to tick bite. group ii was completed of szczecin voivodeship ...199910909488
borrelia burgdorferi sensu lato infection in mosquitoes from szczecin area.the aim of the study was to determine the level of infection in mosquitoes with spirochetes borrelia burgdorferi sensu lato in the woody areas of szczecin. the mosquitoes were collected from may to september 2003. the spirochetes, borrelia burgdorferi s. l., present in mosquitoes were detected in mosquitoes with indirect immunofluorescence assay (ifa) using rabbit anti-borrelia burgdorferi antibodies and goat anti-rabbit igg marked with fluorescein isocyanate (fitc). a total of 1557 females and ...200617044261
[occurrence of genomic species of borrelia burgdorferi sensu lato in populations of ixodes ricinus ticks from west pomerania].the aim of this study was to determine the incidence of borrelia burgdorferi sensu lato infections in ixodes ricinus ticks from recreational areas of the province of west pomerania. ticks (nymphs and adults) were collected between april and october 2000 and 2001 from 10 sampling sites. the presence of the spirochete, borrelia burgdorferi sensu lato, was confirmed with an indirect fluorescence assay (ifa) and the polymerase chain reaction (pcr). in addition, a nested pcr technique was used to det ...200214601491
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