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rickettsia conorii in greece: comparison of a microimmunofluorescence assay and western blotting for seroepidemiology.since 1972, there have been no reports of mediterranean spotted fever (msf) in greece. in 1991, a seroepidemiologic survey was conducted in three rural villages in the province of fokida in central greece, using both an immunofluorescence assay (ifa) and western blot to evaluate the prevalence of specific igg and igm antibodies to rickettsia conorii, and to compare these two techniques. of 254 sera tested by ifa, 148 (58.3%) were positive for igg at a titer > or = 32 and 117 (46.1%) at a titer > ...19938333571
first isolation and identification of rickettsia conorii from ticks collected in the region of fokida in central greece.three different spotted-fever group rickettsiae-rickettsia conorii, r. massiliae, and r. rhipicephali-were detected and identified by pcr-restriction fragment length polymorphism analysis in rhipicephalus ticks collected from domestic animals in the fokida region of greece, where a high seroprevalence of antibodies to r. conorii was previously demonstrated. the infection rate of ticks was 1.6%. moreover, r. conorii was isolated from one rhipicephalus sanguineus tick.200312843081
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