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random amplified polymorphic dna analysis of clinically and environmentally isolated cryptococcus neoformans in nagasaki.we examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of cryptococcus neoformans var. neoformans (serotype a) in the southern japanese prefecture of nagasaki. the random amplified polymorphic dna profiles obtained by using three primers revealed six patterns among 21 clinical isolates and three patterns among 8 environmental isolates. pattern i was the most common (18 of 29 isolates) and was found among isolates obtained throughou ...19958586730
[study on pulmonary cryptococcosis disclosed by chest radiographic screening in nagasaki prefecture].we conducted a retrospective investigation of 20 cases of pulmonary cryptococcosis discovered by chest radiographic screening in nagasaki prefecture among 2,011,577 persons over a period of 9 years, apr. 1989-mar. 1998. eight males and 12 females between 15 and 72 years of age(mean: 46.6 years) were diagnosed as having pulmonary cryptococcosis. the mean detectability of pulmonary cryptococcosis by screening was 0.99 per 10(5) persons: 1.30 for residents, 0.51 for school children and 0.80 for wor ...200011244725
[isolation of cryptococcus neoformans from environments (pigeon excreta) in nagasaki].cryptococcosis is one of the serious deep-seated mycoses in immunocompromised patients, especially those with aids. cryptococcus neoformans ordinarily lives in natural environments such as soil and reproduces in pigeon excreta. it spreads in the air and infects human by inhalation. we isolated c. neoformans from pigeon excreta in hospitals, private houses, parks in nagasaki from october to december in 1994. c. neoformans was isolated from 4 of 8 samples (50%) of pigeon excreta and the isolation ...19957616009
multilocus sequence typing of cryptococcus neoformans in non-hiv associated cryptococcosis in nagasaki, japan.cryptococcosis is primarily caused by two cryptococcus species, i.e., cryptococcus neoformans and c. gattii. both include several genetically diverse subgroups that can be differentiated using various molecular strain typing methods. since little is known about the molecular epidemiology of the c. neoformans/c. gattii species complex in japan, we conducted a molecular epidemiological analysis of 35 c. neoformans isolates from non-hiv patients in nagasaki, japan and 10 environmental isolates from ...201322901045
epidemiology studies of clinical isolates of cryptococcus neoformans of japan by restriction fragment length polymorphism.previous epidemiological studies of cryptococcus neoformans infection in japan showed that only c. neoformans var. neoformans is present and serotype a is the most common with frequencies in excess of 95%. a dna fingerprinting method, using a genomic dna probe (ut-4p), has become available recently which discriminates between individual isolates in a population that are morphologically and serologically indistinguishable. fifty-two serotype a isolates of c. neoformans were obtained from three di ...19947876673
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