| [subclinical infection, clinical infection and vaccination with junin virus]. | | 1976 | 180380 |
| microgeographic distribution of allele frequencies in populations of calomys laucha (rodentia, cricetidae). | the microgeographic differentiation of allelic frequencies was analysed in two population samples of calomys laucha, collected in a grid set in cultivated fields at laguna larga (córdoba province, argentina). one of the samples (n = 79) was obtained in may (autumn), when the population density is at a maximum, and the other (n = 85) in november (spring), at the onset of reproductive activity. fst values for the november sample show significant differences between the six groups over which the sa ... | 1991 | 1880049 |
| hantavirus infection in laboratory and wild rodents in argentina. | serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ... | 1990 | 1981381 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| frequency of rodents in habitats near pergamino, argentina, as related to junín virus. | | 1971 | 5553268 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| [retrospective detection of hantavirus clinical infections in argentina]. | hantavirus activity in rodents and human beings in argentina has been known since the 1980's. in this study, we retrospectively investigated hantavirus infections among argentine hemorrhagic fever (ahf) cases notified between 1987 and 1994, without virological confirmation. igg and igm antibodies to hantavirus were tested by elisa. among 1028 patients included in the study, we found 13 recent infections (1.26%) and 13 remote infections (1.26%). igg antibodies determined in 745 healthy persons li ... | 1996 | 8734923 |
| protective efficacy of a live attenuated vaccine against argentine hemorrhagic fever. ahf study group. | argentine hemorrhagic fever (ahf), caused by the arenavirus junin, is a major public health problem among agricultural workers in argentina. a prospective, randomized, double-blind, placebo-controlled, efficacy trial of candid 1, a live attenuated junin virus vaccine, was conducted over two consecutive epidemic seasons among 6500 male agricultural workers in the ahf-endemic region. twenty-three men developed laboratory-confirmed ahf during the study; 22 received placebo and 1 received vaccine (v ... | 1998 | 9466512 |
| [laboratory evidence of the activity of junin virus in the southeast of cordoba: hypothesis on its epidemiology]. | | 1964 | 14144586 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| rapid diagnosis of argentine hemorrhagic fever by reverse transcriptase pcr-based assay. | argentine hemorrhagic fever (ahf) is an endemo-epidemic disease caused by junín virus. this report demonstrates that a reverse transcriptase (rt) pcr-based assay developed in our laboratory to detect junín virus in whole blood samples is sensitive and specific. the experiments were conducted in a double-blinded manner using 94 clinical samples collected in the area in which ahf is endemic. the rt-pcr-based assay was compared with traditional methodologies, including enzyme-linked immunosorbent a ... | 1995 | 7542268 |
| [new findings on junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in argentina]. | in conjunction with field trials for a vaccine against argentine hemorrhagic fever (ahf), small mammals were trapped during a 28-month period (1 november 1987 to 13 march 1990) in 3 epidemiologically defined areas of the central argentine pampas: northern and central buenos aires provinces were included in the ahf "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern santa fe province is the current high-incidence area for ahf; the nonendemic a ... | 1991 | 7476104 |
| [prevalence of subclinical junin virus infection in a select population of an endemic area of argentinian hemorrhagic fever]. | | 1982 | 7132697 |
| junin virus activity in two rural populations of the argentine hemorrhagic fever (ahf) endemic area. | to determine the prevalence of inapparent infection with junin virus among the rural population and its relation to the clinical disease, a serological study was carried out in two zones of the endemic area of argentine hemorrhagic fever (ahf). from the first appearance of ahf in the zones (1963) and the moment of the survey (1977), 14 years had passed. a total of 695 serum samples were obtained, 540 from córdoba and 155 from buenos aires. of the 695 serum samples, 83 were positive for neutraliz ... | 1983 | 6317799 |
| [immunization against argentina hemorrhagic fever using a strain of attenuated junin virus. 3. serological reactions in volunteers]. | | 1969 | 5802652 |
| [immunization against argentina hemorrhagic fever using a strain attenuated junin virus. ii. imunization of volunteers, clinical and laboratory analysis]. | | 1969 | 5795134 |
| [immunization against argentinian hemorrhagic fever using an attenuated strain of junin virus. i. modified strain of junin virus. immunization of guinea pigs]. | | 1969 | 5346952 |
| geographic patterns of allele frequencies in calomys musculinus reservoir-host of junin virus. | | 1986 | 3543610 |
| [junin virus activity in humans and rodents of non-endemic areas of the province of buenos aires]. | | 1985 | 3020341 |
| experimental infection of akodon molinae (rodentia, cricetidae) with junín virus. | experimental infection with the xj-clone 3 strain of junín virus in laboratory bred akodon molinae, a cricetid rodent inhabiting the borders of endemic argentine hemorrhagic fever areas, was studied. suckling animals inoculated intracerebrally proved sensitive and became chronically infected. sixty percent of the rodents showed neurologic involvement, with mortality reaching 60%. virus was recovered from the brain at 7, 15, 21, 37, and 57 days postinfection (pi). by immunofluorescence (if), vira ... | 1986 | 3009705 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |
| a longitudinal study of junin virus activity in the rodent reservoir of argentine hemorrhagic fever. | we monitored junin virus (jv) activity in rodent populations for 30 months at seven mark-recapture grids located in agricultural fields and adjacent roadsides and fence lines in endemic and nonendemic areas of argentine hemorrhagic fever. blood and oral swabs taken from rodents captured at five-week intervals were analyzed by enzyme-linked immunosorbent assay for jv antigen (ag). calomys laucha and c. musculinus were the most frequently captured rodents, making up 47% and 22% of captures, respec ... | 1992 | 1335214 |
| clinical and epidemiological patterns of argentine haemorrhagic fever. | the epidemiology of argentine haemorrhagic fever (ahf) is closely related to cricetine rodents acting as natural hosts of junin virus. the endemo-epidemic area, which has increased 5 times since the disease was first recognized 15-20 years ago, is located in a densely populated region of argentina. it has been shown that the virus of lcm is active in humans and rodents of the ahf endemic area; this demonstrates the simultaneous presence of two arenaviruses pathogenic for man in a given geographi ... | 1975 | 1085212 |
| [candid#1 vaccine against argentine hemorrhagic fever produced in argentina. immunogenicity and safety]. | a clinical study in 946 human volunteers was done to compare candid #1 vaccine manufactured in argentina with the vaccine produced in usa that had been previously used. the efficacy was evaluated using immunogenicity measured by the detection of neutralizing antibodies as a subrogate marker. safety was evaluated comparing the rate of adverse events. both vaccines showed a comparable rate of seroconversion, slightly higher than the efficacy estimated from previous studies (95.5%). there were no s ... | 2010 | 20529769 |
| treatment of argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a rodent-borne illness caused by the arenavirus junin that is endemic to the humid pampas of argentina. ahf has had significant morbidity since its emergence in the 1950s, with a case-fatality rate of the illness without treatment between 15% and 30%. the use of a live attenuated vaccine has markedly reduced the incidence of ahf. present specific therapy involves the transfusion of immune plasma in defined doses of neutralizing antibodies during the prodromal ... | 2008 | 18054395 |
| [preclinical assay of candid #1 vaccine against argentine hemorrhagic fever made in argentina]. | candid #1 vaccine against argentine hemorrhagic fever produced in usa versus lots of the same vaccine made in argentina were compared in guinea pigs regarding safety, immunogenicity and protective efficacy against a challenge with pathogenic junin virus. lots no exp 3, 7a and 8a of argentine origin as well as lot tsi 5-1-92 from usa were inoculated in guinea pigs of 250-400 g in two consecutive assays. ten animals inoculated with saline performed as normal controls in each experiment. parameters ... | 2005 | 16193711 |
| evaluation of chemical and antiviral properties of essential oils from south american plants. | the essential oils of seven aromatic plants from córdoba, san luis and san juan provinces (argentina) were isolated by steam distillation and analysed by a gas chromatography/mass spectrometry technique. the oils were screened for cytotoxicity and in vitro inhibitory activity against herpes simplex virus type 1 (hsv-1), dengue virus type 2 (denv-2) and junin virus (junv) by a virucidal test. the oils showed a variable virucidal action according to the virus. the better relationship between cytot ... | 2005 | 16130522 |
| recent range expansion and low levels of contemporary gene flow in calomys musculinus: its relationship with the emergence and spread of argentine haemorrhagic fever. | the geographic distribution of haplotype diversity in the rodent calomys musculinus, sampled from 16 wild populations of argentina, was analysed on two geographical scales. the species is the natural reservoir of the junin virus, the etiological agent of the argentine haemorrhagic fever (ahf). in all, 24 composite haplotypes were recognised in the mtdna d-loop region. haplotypes 1 and 2, internal in the network, were the most frequent and were present in almost all populations. the absence of la ... | 2004 | 15316554 |
| restricted gene flow in calomys musculinus (rodentia, muridae), the natural reservoir of junin virus. | random amplified polymorphic dna polymerase chain reaction (rapd-pcr) markers were used to evaluate the relative contribution of gene flow as a determinant of the population genetic structure of the wild rodent calomys musculinus (the reservoir of argentine hemorrhagic fever [ahf]) in central argentina. one hundred eighty-seven individuals from 13 populations (9 of them from the endemic zone of ahf and 5 from areas outside it) were analyzed using 78 polymorphic rapd loci. genetic variation withi ... | 2003 | 14691315 |
| gene flow among calomys musculinus (rodentia, muridae) populations in argentina. | calomys musculinus is a sigmodontinae rodent inhabiting periodically disturbed habitats in the central eastern plains of argentina. it is the natural reservoir host of junin virus, the etiological agent of argentine hemorrhagic fever (ahf). in order to analyze the levels of gene flow among populations of this species, allozymic variability at 26 loci was studied in 291 individuals from the endemic zone of ahf and localities outside it. all populations showed high levels of polymorphism (he betwe ... | 2002 | 11990761 |
| [neutralization test for lymphocytic choriomeningitis virus for distinguishing between two arenavirus infections in argentina]. | the active coexistence of two pathogenic arenaviruses, junin (junv) and lymphocytic choriomeningitis (lcmv), in the same region of argentina, has been known since the early 70's, and records of clinical and subclinical human infections by one and/or the other agent have been continuously produced for the last 25 years. anti-lcmv antibody is currently searched only by indirect immunofluorescence, a test that shows cross reactions among a number of arenaviruses yielding, in the cases of lcmv and j ... | 2001 | 11833256 |
| genetic diversity of the junin virus in argentina: geographic and temporal patterns. | rna was purified from 39 strains of cell-cultured junin virus (jun) from central argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory jun strains, xj cl3 and xj #44. jun-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (gp1) gene. genetic diversity among jun strains studied was up to 13% at the nt level and up to 9% at th ... | 2000 | 10873755 |
| clinical case definitions for argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a potentially lethal infection in argentina. the case-fatality ratio is >15%, but treatment reduces the mortality rate to <1%. diagnosis is based on clinical and laboratory criteria, but no case definition has been validated. a chart review was conducted for patients hospitalized with suspected ahf. individuals with a fourfold rise in antibody titer were classified as cases. the combination of a platelet count of <100,000/mm3 and a white blood cell (wbc) coun ... | 1999 | 10452640 |
| virucidal activity and chemical composition of essential oils from aromatic plants of central west argentina. | the essential oils of seven aromatic plants from central west argentina were isolated by steam distillation and analyzed by a gas chromatography/mass spectrometry technique. the oils were screened for cytotoxicity and in vitro inhibitory activity against herpes simplex virus type 1 (hsv-1), dengue virus type 2 (denv-2) and junin virus (junv). the oils showed a variable virucidal action according to the virus. junv was the least susceptible virus in comparison with hsv-1 and denv-2. the better re ... | 2010 | 20839642 |
| argentine hemorrhagic fever vaccines. | argentine hemorrhagic fever (ahf), an acute disease caused by junin virus (junv, arenaviridae), has been an important issue to public health in argentina since the early 1950s. the field rodent calomys musculinus is junv natural reservoir and human disease is a consequence of contact with infected rodents. a steady extention of ahf endemic area is being observed since the first reports of the disease. important achievements have been made in: (a) improvement of methods for the etiological diagno ... | 2011 | 21451263 |
| viral diversity of jun+¡n virus field strains. | the argentine hemorrhagic fever, an endemic disease present in a much of argentina, is caused by the jun+¡n virus (junv). currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (xj13, xj#44 and candid#1), as well as mc2 (rodent isolate) and iv4454 (human isolate). in this article, we report sequence information on two fragments of genomic segment s of viral isolates from the endemic area. a nested-rt-pcr was used to amplify discre ... | 2011 | 21689697 |