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seroepidemiological studies of cutaneous leishmaniasis in the campeche state of méxico.seroepidemiological studies of cutaneous leishmaniasis were carried out in 169 individuals in a rural area of the campeche state of méxico. fifty showed cutaneous lesions suggestive of leishmaniasis, 70% were parasite positive and 96% skin test positive. an overall 40% positivity to skin test with montenegro's antigen was found. most of the affected individuals were males from 11 to 30 years-old. antibodies were determined by immunofluorescent antibody test (ifa) and by western blot. two antigen ...19979302408
isoenzyme characterization of leishmania isolated from human cases with localized cutaneous leishmaniasis from the state of campeche, yucatan peninsula, mexico.seventy-five isolates from the state of campeche, mexico, an area endemic for localized cutaneous leishmaniasis (lcl), were characterized by isoenzyme markers (glucose phosphate isomerase, mannose phospate isomerase, nucleoside hydrolase, phosphoglucomutase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase). seventy (93.3%) were identified as leishmania (leishmania) mexicana and 5 (6.7%) as l. (viannia) braziliensis. this is the first report of authochthonus human lcl caus ...19989574789
identification of mexican leishmania species by analysis of pcr amplified dna.leishmania parasites isolated into culture from patients with lcl or dcl from four different mexican states were characterised using polymerase chain reaction (pcr), hybridisation with specific probes, and isoenzymes. pcr of the parasites showed that 10 of 11 of those isolates were members of the mexicana complex. this was confirmed in seven cases by isoenzymes. restriction enzyme digests of pcr products of mexican isolates showed the isolates to be different from the l.(l.) mexicana reference s ...19989821463
molecular probes and the polymerase chain reaction for detection and typing of leishmania species in mexico.leishmaniasis in mexico is a public health problem because all the clinical forms have been recorded in most mexican states. we studied patients showing clinical symptoms of any form of leishmaniasis, from several endemic areas. bone marrow samples, aspirates or skin biopsies were taken and deoxyribonucleic acid (dna) was extracted and amplified by the polymerase chain reaction (pcr) with universal primers ajs1 and deb8, specific for the leishmania subgenus leishmania. the pcr products were then ...200212055821
study of cutaneous leishmaniasis in the state of campeche (yucatan peninsula), mexico, over a period of two years.to study cutaneous leishmaniasis (cl), in the calakmul municipality of the campeche state, during two years.201625629280
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