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diagnosis of cutaneous and mucocutaneous leishmaniasis in colombia: a comparison of seven methods.seven methods of diagnosing leishmaniasis were compared in 177 patients presenting with lesions of the skin (165) or mucosa (12) in tumaco and cali, colombia. the three methods of visualizing amastigotes in tissue samples (histological staining of tissue sections, impression smears of punch biopsies, and smears of dermal scraping from slits in the lesion margins) were less sensitive than the four leishmania isolation methods (aspiration of lesion border cultured in biphasic media, aspirate inocu ...19872437815
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