characterization of leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in santa catarina state, southern brazil.four leishmania sp. samples were isolated from autochthonous human cases of american cutaneous leishmaniasis (acl) in santa catarina state, southern brazil. these strains were characterized using indirect immunofluorescence with a panel of leishmania-specific monoclonal antibodies (mabs), and by pcr amplification and hybridization assay of the mini-exon gene with group specific probes. the results obtained with the mabs were in agreement with the genetic marker. two isolates (mhom/br/89/jsc89-h1 ...200010643912
lutzomyia (nyssomyia) whitmani s.l . (antunes & coutinho, 1939)(diptera: psychodidae: phlebotominae): geographical distribution and the epidemiology of american cutaneous leishmaniasis in brazil--mini-review.the aim of the present study, in view of the widespread geographical distribution of lutzomyia (nyssomyia) whitmani s.l. in brazil, in close association with the regions of transmission of leishmania (viannia) braziliensis and l. (v.) shawi, is to investigate the distribution of this sand fly species and american cutaneous leishmaniasis (acl) in relationship to vegetation and landscape ecology throughout brazil. thematic maps were elaborated by the mapinfo programme, giving information on the sp ...200717426877
natural infection of nyssomyia neivai (pinto, 1926) (diptera: psychodidae, phlebotominae) by leishmania (viannia) spp. in brazil.a study of the natural infection of phlebotomine sand flies by leishmania (viannia) was conducted in a focus of cutaneous leishmaniasis in piçarras, on the northeastern coast of the brazilian state of santa catarina. in total, 562 female nyssomyia neivai were collected by miniature light traps near houses, separated in 61 pools and examined by pcr and southern blot hybridization. eight pools, four of them from the same light trap/night, were positive. this is the first finding of natural infecti ...200919178921
multilocus sequence analysis for leishmania braziliensis outbreak investigation.with the emergence of leishmaniasis in new regions around the world, molecular epidemiological methods with adequate discriminatory power, reproducibility, high throughput and inter-laboratory comparability are needed for outbreak investigation of this complex parasitic disease. as multilocus sequence analysis (mlsa) has been projected as the future gold standard technique for leishmania species characterization, we propose a mlsa panel of six housekeeping gene loci (6pgd, mpi, icd, hsp70, mdhmt ...201424551258
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