cutaneous leishmaniasis of man due to leishmania (viannia) shawi lainson, de souza, póvoa, ishikawa & silveira, in pará state, brazil.leishmania (viannia) shawi lainson, braga, de souza, póvoa, ishikawa & silveira, 1989, was originally recorded from monkeys (cebus apella and chiropotes satanas), sloths (choloepus didactylus and bradypus tridactylus) and coatis (nasua nasua) and the sandfly, lutzomyia whitmani. with a panel of leishmania specific monoclonal antibodies, it was found that 30.5% of the leishmania strains from patients, who had contracted cutaneous leishmaniasis in pará state, were similar, but not identical to l. ...19911822654
cutaneous leishmaniasis in the amazon region: natural infection of the sandfly lutzomyia ubiquitalis (psychodidae: phlebotominae) by leishmania (viannia) lainsoni in pará state, brazil. 19911842393
[cutaneous leishmaniasis in amazonia: isolation of leishmania (viannia) lainsoni from the rodent agouti paca (rodentia: dasyproctidae), in the state of pará, brazil].the isolation of leishmania (v.) lainsoni is recorded for the first time from a wild animal, the rodent agouti paca (rodentia: dasyproctidae), from pará state, north brazil. isolates of the parasite were made from apparently normal skin of 3 specimens of this rodent captured on the island of tocantins, in the municipality of tucuruí, an area subsequently flooded in the formation of the lake associated with the tucuruí hydroelectric dam. no isolations were made from the viscera. identification of ...19911843391
characterization and classification of leishmanial parasites from humans, wild mammals, and sand flies in the amazon region of brazil.ninety-four leishmanial isolates from the brazilian amazon region (amapá, amazonas, pará, and rondônia) were identified and classified using specific monoclonal antibodies and an indirect radioimmunoassay (serodeme analysis); eighty-two were also characterized by enzyme electrophoresis (zymodeme analysis), the results of which were subjected to a numerical phenetic analysis. six isolates from humans (3), didelphis marsupialis (1), lutzomyia olmeca nociva (1), and lu, reducta (1) showed reactivit ...19911858968
leishmania (viannia) shawi sp. n., a parasite of monkeys, sloths and procyonids in amazonian brazil.leishmania (viannia) shawi sp. n., is described from the monkeys cebus apella and chiropotes satanus, the sloths choloepus didactylus and bradypus tridactylus, the procyonid nasua nasua, and the phlebotomine sandfly lutzomyia whitmani, all from primary forest in the state of pará, north brazil. l. (v.) shawi is variably distinguished from all other known species within the subgenus viannia by a combination of biological, biochemical and serological characters, as revealed by studies on morpholog ...19892504099
leishmania (viannia) naiffi sp. n., a parasite of the armadillo, dasypus novemcinctus (l.) in amazonian brazil.a new leishmanial parasite, leishmania (viannia) naiffi sp. n., is described from the nine-banded armadillo, dasypus novemcinctus (edentata: dasypodidae), from para state, north brazil. the parasite grows luxuriantly in diffco blood-agar medium (b47), but poorly in the skin of intradermally inoculated hamsters. a comparison of isoenzyme profiles by starch gel electrophoresis separates the parasite from l. (v) braziliensis and l. (v.) guyanensis by the enzymes asat, alat, pgm, gpi, g6pd, pep, mpi ...19892930120
leishmaniasis in brazil. xxv. sandfly vectors of leishmania in pará state, brazil.leishmania of the braziliensis complex were isolated from various members of the squamiventris series sandflies, including psychodopygus chagasi (costa lima), p.s.maripaensis (ready et al.), p.s.squamiventris (lutz & neiva), and also p.ayrozai (barreto & coutinho) and lutzomyia umbratilis ward & fraiha. three different serodemes of le.b.guyanensis floch were isolated from lu.anduzei rozeboom, lu.umbratilis and lu.whitmani antunes & coutinho, simultaneously captured in the same area. unidentified ...19872979555
leishmaniasis in brazil: xxiii. the identification of leishmania braziliensis braziliensis in wild-caught neotropical sandflies using monoclonal antibodies.using the indirect immunofluorescence test natural flagellate infections of wild-caught sandflies, from the serra dos carajás region of pará state, brazil, were identified by sequentially staining smears made from the infected flies with monoclonal antibodies. with normal methods of isolation 30% of the infections were identifiable, but when monoclonal antibodies specific to leishmania braziliensis braziliensis were used a further 26% were identified. the staining of organisms in smears of natur ...19873445325
dermal leishmaniasis in the amazon region of brazil: leishmania (viannaia) lainsoni sp.n., a new parasite from the state of pará. 19873506634
leishmaniasis in brazil. xxiv. natural flagellate infections of sandflies (diptera: psychodidae) in pará state, with particular reference to the rôle of psychodopygus wellcomei as the vector of leishmania braziliensis braziliensis in the serra dos carajás.between july 1983 and december 1984 natural flagellate infections were found in 114 (1%) of 11,586 female phlebotomine sandflies (diptera: psychodidae) of 21 species. a further 1084 females of 17 other species were not infected. identification of the organisms on a number of occasions confirms the exclusive parasite/vector relationship of leishmania mexicana amazonensis/lutzomyia flaviscutellata and le. braziliensis braziliensis/psychodopygus "wellcomei". undescribed or unidentified leishmania s ...19873686628
identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies.five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ...19873826486
flagellate infections of brazilian sand flies (diptera: psychodidae): isolation in vitro and biochemical identification of endotrypanum and leishmania.flagellate infections were found in 1,063 of 18,895 sand flies collected in the states of amazonas, pará, rondonia and acre, brazil. infection rates were 13.4% (species group shannoni); 7.5% (subgenus nyssomyia); 6.7% (subgenus lutzomyia series cruciata); 0.5% (genus psychodopygus) and 3.1% for other sand flies (various subgenera). leishmania braziliensis guyanensis and l. mexicana amazonensis were isolated, respectively, from the known vectors, lutzomyia umbratilis and l. flaviscutellata. singl ...19853938924
leishmaniasis in brazil: xvii. enzymic characterization of a leishmania from the armadillo, dasypus novemcinctus (edentata), from pará state.a comparison of enzyme profiles, by starch-gel electrophoresis, has distinguished a leishmania of armadillos (dasypus novemcinctus), from pará state, north brazil, from leishmania braziliensis braziliensis, l. braziliensis guyanensis, l. mexicana amazonensis, l. donovani sensu lato (from bahia, brazil), and l. hertigi deanei. the parasite was separated from l. b. braziliensis and l. b. guyanensis by 8 of the 14 enzymes used (asat, alat, pgm, gpi, g6pd, pep, mpi and gd), although differences in t ...19827164148
leishmaniasis in brazil: xvi. isolation and identification of leishmania species from sandflies, wild mammals and man in north para state, with particular reference to l. braziliensis guyanensis causative agent of "pian-bois".a total of 125 wild mammals (14 different species) were examined for evidence of infection with leishmania in an area of primary forest highly endemic for "pian-bois", due to leishmania braziliensis guyanensis, in north pará state, brazil. parasites isolated were characterized biologically, and biochemically on enzymic profiles. l. b. guyanensis was isolated from the viscera of one lesser anteater (tamandua tetradactyla) and one opossum (didelphis marsupialis), and the skin of one rodent (proech ...19817324128
leishmania (viannia) braziliensis: genetic relationships between strains isolated from different areas of brazil as revealed by dna fingerprinting and rapd.leishmania (viannia) braziliensis isolates from two different geographic areas in brazil were studied by dna fingerprinting with the 33.15 multilocal probe and pcr with arbitrary primers (random amplification of polymorphic dna-rapd). the genetic distance of strains was measured by band sharing. the results showed that the strains isolated in minas gerais, southeastern brazil, are very different from those isolated in pará, northern brazil. strains from minas gerais constituted a relatively homo ...19957758549
psychodopygus complexus, a new vector of leishmania braziliensis to humans in pará state, brazil.this paper reports the finding of 4 specimens of psychodopygus complexus, captured in the paragominas region of pará state, brazil, naturally infected with trypanosomatids that were positively identified as leishmania (viannia) braziliensis. attention is drawn to the importance of this species as a vector since it is highly anthropophilic and has a very extensive geographical distribution in the lower amazon region of pará state south of the amazon river, including the island of marajó.19968761563
an outbreak of cutaneous leishmaniasis among soldiers in belém, pará state, brazil, caused by leishmania (viannia) lindenbergi n. sp. a new leishmanial parasite of man in the amazon region.eight cases of cutaneous leishmaniasis are recorded among soldiers of the brazilian forest infantry stationed in belém, state of pará, north of brazil. the infections, all acquired during manoeuvres in nearby degraded primary forest, are attributed to a new member of the subgenus viannia, leishmania (v.) lindenbergi n. sp. a further infection by this parasite was encountered in a woman, who lived very close to the same piece of forest. the new parasite has been characterised and differentiated f ...200211938695
genetic variation in populations of leishmania species in brazil.the population structure of strains of leishmania (viannia) braziliensis sensu lato from pará state and paraná state in brazil, of l. (v.) shawi and l. (leishmania) amazonensis from pará state, and the relationships of type strains of the subgenera l. (viannia) and l. (leishmania) were examined by the random-amplified polymorphic deoxyribonucleic acid (rapd) technique. four different primers (m13-40, qg1, l15996 and delta gt11r) were used. the bands were analysed using the neighbor-joining (nj) ...200212055823
leishmania (viannia) utingensis n. sp., a parasite from the sandfly lutzomyia (viannamyia) tuberculata in amazonian brazil.a leishmanial parasite isolated in 1977 from a specimen of the sandfly lutzomyia tuberculata from pará state, amazonian brazil, has been characterized following its comparison with other species of leishmania from the same region, using isoenzyme profiles, monoclonal antibodies and characterization of the miniexon gene repeat, using the polymerase chain reaction technique (pcr). it is described here under the name of leishmania (viannia) utingensis n. sp.200312847917
characterization in vivo and in vitro of a strain of leishmania (viannia) shawi from the amazon region.the present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro, of leishmania (viannia) shawi, a parasite identified in the amazon region, pará state, in 1989. we demonstrated that promastigotes in the stationary (stat) growth phase are more resistant to complement lysis than in the logarithmic (log) growth phase. ultrastructural analyses and imidazol technique showed accumulation of lipids in stat growth phase pro ...200919567230
etiology of cutaneous leishmaniasis and anthropophilic vectors in juruti, pará state, a preliminary study in juruti, a mining municipality in western pará state, brazil, 12 out of 21 patients suspected of presenting cutaneous leishmaniasis showed positive pcr (ssurdna and g6pd): leishmania (viannia) braziliensis (9/12; 75%) and l. (v.) sp. (3/12; 25%). entomological studies in the same location revealed the presence of 12 different phlebotomine species (n =105). one of the most common species was lutzomyia (psychodopygus) complexa (17%) which is both highly anthropophilic and ...200919851628
leishmania (viannia) braziliensis identification by pcr in the state of para, brazil.the incidence of cutaneous leishmaniasis (cl) is increasing and there is limited surveillance of leishmania species throughout the world. we identified the species associated with cl in a region of amazonia, an area recognized for its leishmania species variability. clinical findings were analyzed and correlated with the species identified in 93 patients. pcr assays were based on small subunit ribosomal dna (ssu-rdna) and g6pd, and were performed in a laboratory located 3,500km away. leishmania ...201121241996
mucosal leishmaniasis caused by leishmania (viannia) braziliensis and leishmania (viannia) guyanensis in the brazilian amazon.leishmania (viannia) braziliensis is a parasite recognized as the most important etiologic agent of mucosal leishmaniasis (ml) in the new world. in amazonia, seven different species of leishmania, etiologic agents of human cutaneous leishmaniasis, have been described. isolated cases of ml have been described for several different species of leishmania: l. (v.) panamensis, l. (v.) guyanensis and l. (l.) amazonensis.201121408116
identification and biological characterization of leishmania (viannia) guyanensis isolated from a patient with tegumentary leishmaniasis in goiás, a nonendemic area for this species in brazil.the aim of this study was to characterize clinical field isolates of leishmania spp. obtained from patients with american tegumentary leishmaniasis (atl) who live in goiás state, brazil. the presumed areas of infection were in goiás, tocantins, and pará states. three isolates of parasites were identified as l. (viannia) braziliensis and one as l. (v.) guyanensis. the in vitro growth profiles were found to be similar for all parasites. nevertheless, in c57bl/6 mice, l. (v.) guyanensis infection w ...201526583102
phenotypic characterization of leishmania spp. causing cutaneous leishmaniasis in the lower amazon region, western pará state, brazil, reveals a putative hybrid parasite, leishmania (viannia) guyanensis × leishmania (viannia) shawi shawi.we phenotypically characterized 43 leishmanial parasites from cutaneous leishmaniasis by isoenzyme electrophoresis and the indirect immunofluorescence antibody test (23 mcabs). identifications revealed 11 (25.6%) strains of leishmania (v.) braziliensis, 4 (9.3%) of l. (v.) shawi shawi, 7 (16.3%) of l. (v.) shawi santarensis, 6 (13.9%) of l. (v.) guyanensis and l. (v.) lainsoni, 2 (4.7%) of l. (l.) amazonensis, and 7 (16.3%) of a putative hybrid parasite, l. (v.) guyanensis/l. (v.) shawi shawi. m ...201425083790
natural leishmania (viannia) infections of phlebotomines (diptera: psychodidae) indicate classical and alternative transmission cycles of american cutaneous leishmaniasis in the guiana shield, brazil.from 1996 to 1999 multi-trapping methods (center of diseases control, cdc) light traps, light-baited shannon traps, and aspiration on tree bases) were used to study the phlebotomine fauna of the "serra do navio" region of the brazilian state of amapá, which is part of the guiana shield. fifty-three species were identified among 8,685 captured individuals. the following species, associated with the transmission of american cutaneous leishmaniasis in amazonian brazil, were captured: nyssomyia umbr ...201728508745
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