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[genotype distribution of enterovirus a species isolated in shandong province, china].in order to study the genotypes and molecular evolution of human enterovirus (hev) a species in shandong province, stool samples were collected from afp and hfmd patients in shandong province and virus isolation was performed. reverse transcription-polymerase chain reactions (rt-pcr) specific for ev71 and cva16 were performed with the virus isolates from hfmd patients. positive isolates were selected for entire vp1 coding gene amplification and sequencing. isolates with negative pcr results and ...200920077929
an outbreak of hand, foot, and mouth disease associated with subgenotype c4 of human enterovirus 71 in shandong, china.an outbreak of hand, foot, and mouth disease (hfmd) included 1149 people in linyi city, shandong province, china, in 2007: three children died.200919269888
isolation and genomic characterization of three enterovirus 90 strains in shandong, china.enterovirus 90 (ev90) is a newly identified serotype of the species human enterovirus a, and few nucleotide sequences of ev90 are available. in this study, three ev90 strains were isolated from acute flaccid paralysis (afp) cases in shandong province, china, in 2001 and 2003. sequence analysis revealed 96.7-98.0 % vp1 nucleotide identity among themselves and 77.7-92.3 % to other ev90 strains. complete genome analysis provided evidence of recombination in the non-capsid coding region of strain 01 ...201323081679
[identification and genetic characterization of coxsackieviruses a2, 6, 8 and 12 isolated in shandong province].in previous study, molecular typing method was performed to identify human enteroviruses (hevs) isolates collected from acute flaccid paralysis (afp) cases from 1989 to 2011 and hand, foot and mouth disease (hfmd) patients, and 8 hev-a serotypes were identified. in order to explore the genotypes and molecular evolution characteristics of hev-a in shandong province, viral rna of the remaining isolates was extracted and entire vp1 coding region was amplified, sequenced and identified with hev-a pr ...201223233927
the complete genome sequence of an enterovirus 76 isolate in china reveals a recombination event.enterovirus 76 (ev76) is a new member of species human enterovirus a (hev-a). so far, the only complete genome sequence of the prototype strain from france has been available. in this study, we determined the complete nucleotide sequence of strain 04360, isolated from an acute flaccid paralysis patient in shandong province, china in 2004. sequence analysis revealed 80.7-94.7% vp1 nucleotide identity to other ev76 strains and provided evidence of recombination with other types of hev-a in the p2 ...201121755310
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