| bovine herpesvirus type 1 abortions detected by a semi-nested pcr in brazilian cattle herds. | bovine herpesvirus type 1 (bhv-1) was investigated by a semi-nested polymerase chain reaction (sn-pcr) and by mdbk cell culture virus isolation in organ fragments from 55 aborted fetuses collected from beef and dairy cattle herds with history of reproductive problems in the north of paraná state, brazil. a 425 bp amplicon of the bhv-1 glycoprotein d gene was detected in 14 (25.4%) aborted fetuses. bhv-1 was isolated in mdbk cells from the tissue of 5 (9.1%) fetuses. the specificity of positive r ... | 2004 | 15894030 |