| livestock farmers' perception and epidemiology of bovine trypanosomosis in kwale district, kenya. | we did cross-sectional surveys in kwale district, kenya to determine the epidemiology of bovine trypanosomosis and livestock owners' perceptions of the disease. the surveys involved relative importance of trypanosomosis, examination of the current disease constraints, current control practices and drug-use patterns. informal meetings were held with farmers and cattle census undertaken. tsetse-fly densities and trypanosomosis prevalences in cattle were determined. a total of 132 farmers were inte ... | 2007 | 17324481 |
| identification of trypanosomes in glossina pallidipes and g. longipennis in kenya. | the polymerase chain reaction (pcr) was used to identify trypanosomes in glossina pallidipes and g. longipennis caught in kenya. of 3826 flies dissected, 188 (4.9%) were parasitologically positive overall. the infection rate in g. pallidipes was 5.7% (187 of 3301 flies), but only one of 525 g. longipennis was infected (infection rate 0.2%). there was a higher infection rate in female g. pallidipes flies than male flies (chi(2) = 18.5, p < 0.001) and odds ratio = 2.5 (95% 1.6, 3.7). the infected ... | 2004 | 15019587 |
| a comparison of susceptibility of two allopatric populations of glossina pallidipes for stocks of trypanosoma congolense. | a colony of glossina pallidipes austen which originated from nguruman, rift valley province, kenya, was significantly more susceptible to infection (19.3%) with a stock of trypanosoma congolense broden isolated from g. pallidipes in nguruman than a colony of the same species which originated from shimba hills, coast province, kenya (5.6%). male g.pallidipes from nguruman were significantly more susceptible than females to this t.congolense stock whilst the susceptibility of both sexes of g.palli ... | 1993 | 8268493 |
| trypanosoma congolense: host responses following tsetse transmitted infection of kilifi isolates in goats. | east african x galla goats, when infected with trypanosoma congolense isolates from the kilifi area of kenya by glossina morsitans centralis, did not develop the characteristic chancre reaction at the bite sites, whereas bites of tsetse infected with the cloned t. congolense il.1180 from serengeti, tanzania, resulted in chancres in the same goats. histological changes could not be observed in skin biopsies collected 8 or 9 days after infection with kilifi isolates. however, all goats became para ... | 1987 | 3582569 |
| genetically discrete populations of trypanosoma congolense from livestock on the kenyan coast. | twenty-seven stocks of nannomonas trypanosomes isolated from livestock in 1982 on a ranch at kilifi on the kenyan coast were characterized by isoenzyme electrophoresis and by the abilities of the parasite's dna to hybridize to two repetitive sequence dna probes. all the kilifi stocks which were examined had isoenzyme patterns which were markedly different from the 75 patterns previously described from 78 stocks of trypanosoma congolense. on average only 15% of the enzyme bands present in the kil ... | 1988 | 3405633 |
| characterization of trypanosoma congolense serodemes in stocks isolated from cattle introduced onto a ranch in kilifi, kenya. | a herd of 20 cattle was introduced on a ranch in kilifi, coast province of kenya, where they were in contact with glossina austeni for 6 months. in total, 65 trypanosome isolates were made from these animals. examination of the isolates revealed that 61 were trypanosoma congolense and 4 were t. theileri. out of the 61 t. congolense isolates, 55 were successfully passaged and cloned in mice to provide trypanosome populations for further analyses. the stocks and their clones were inoculated into g ... | 1987 | 3295687 |
| the chromosome profiles of trypanosoma congolense isolates from kilifi, kenya and their relationship to serodeme identity. | chromosomal dna from 117 trypanosoma congolense clones from 54 stocks, isolated from cattle introduced onto a ranch in kilifi in the coastal area of kenya, was fractionated by the orthogonal field alternation gel electrophoresis technique. the technique resolved chromosomes in the size range of 100 kb-1 mb. the chromosome profile for cloned trypanosome populations was relatively stable with regard to number and size of the chromosome bands following transmission in mice, cattle, goats or tsetse ... | 1988 | 3173429 |
| epidemiology of animal trypanosomiasis on a cattle ranch in kilifi, kenya. | a study of the epidemiology of animal trypanosomiasis was carried out on a 2500 ha cattle ranch, with a history of trypanosomiasis, in the coast province of kenya in 1982. the tsetse survey on the ranch revealed one breeding focus of glossina austeni in a thicket of approximately 50 ha. trypanosomes were detected in 20% of the 46 dissected tsetse. during the study period of 9 months, 0.8% of the 3315 samples collected from 2300 ayrshire x sahiwal crossbred cattle were found infected with trypano ... | 1987 | 2884841 |