| molecular detection of ehrlichia ruminantium infection in amblyomma variegatum ticks in the gambia. | in west africa, losses due to heartwater disease are not known because the incidence/prevalence has not been well studied or documented. to develop a diagnostic tool for molecular epidemiology, three pcr-based diagnostic assays, a nested pcs20 pcr, a nested map1 pcr and a nested reverse line blot (rlb) hybridization assay, were evaluated to determine their ability to detect infection in vector ticks, by applying them simultaneously to a. variegatum field ticks to detect ehrlichia ruminantium, th ... | 2007 | 17476576 |
| point seroprevalence survey of ehrlichia ruminantium infection in small ruminants in the gambia. | using the map1-b enzyme-linked immunosorbent assay, we tested 1,318 serum samples collected from sheep and goats at 28 sites in the five divisions of the gambia to determine the ehrlichia ruminantium seroprevalence rates and to assess the risk for heartwater. about half (51.6%) of 639 sheep were positive, with seroprevalence rates per site varying between 6.9% and 100%. the highest seroprevalence was detected in the western part of the country (88.1% in the western division and 62.1% in the lowe ... | 2005 | 15817758 |