otomycosis in western nigeria.otomycosis is a recognized clinical entity in the tropical regions of the world. however, there is scanty information on this disease in some parts of sub-saharan africa. the aim of this study was to determine the prevalence and pattern of etiological agents of otomycosis in western nigeria. medical records of patients with otomycosis seen in the otorhinolaryngology department of the university college hospital, ibadan from 1996-2005 were reviewed for all essential clinical data. of the 5784 pat ...200818076598
prevalence of otomycosis in malnourished children in edo state, nigeria.out of the total number at 200 suspected cases of otomycoses consisting of 40 malnourished and 160 apparently healthy children examined in this study between the months of july and august in edo state, 64 cases (32%) were identified to be of fungal aetiology on the basis of positive culture and careful microscopic examination. the state at protein energy malnourishment was deterwined using physicians' comments in their case files. the fungal agents isolated were aspergillus niger 28 (43.8%); a. ...19979646512
isolation and characterisation of antibiotic producing microorganisms in composted nigerian soil.compost samples obtained from different locations within the premises of the university of lagos were analysed to determine the presence and types of antibiotic-producing bacteria, fungi and actinomycetes using nutrient agar, potato dextrose agar and starch casein nitrate agar respectively as culture media. a variety of bacteria were isolated and these included staphylococcus aureus, b. subtilis, b. pumilis, b. lactesporus, b. megaterium, b. pulvifaciens, b. licheniformis, streptococus spp., cor ...200415847340
pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices.microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ...200415691161
mycoflora and occurrence of aflatoxin b1 in dried yam chips from markets in ogun and oyo states, nigeria.seventy-six samples of dried yam chips locally called elubo isu were purchased in 2000 from markets in ogun and oyo states of southwestern nigeria. the samples were assessed for ph, moisture content, associated fungi and aflatoxin b1 contamination. the ph of samples ranged from 5.6 to 6.1,while the moisture contents varied from 6.8 to 14.5% in ogun samples, and 7.1 to 13.6% in samples from oyo. aspergillus and penicillium were the two prevalent genera of fungi, and the number of colony forming u ...200415008353
isolation and characterization of microorganisms from instruments used by pedicurists operating within lagos metropolis, nigeria.eight bacterial and five fungal species were isolated from swab samples taken from instruments used by pedicurists operating at three different sites in lagos, nigeria. the bacterial isolates included micrococcus luteus, micrococcus roseus, staphylococcus epidermidis, staphylococcus aureus, hafnia spp, shigella spp, bacillus subtilis and bacillus spp. the five fungal isolates were identified as aspergillus niger, aspergillus flavus, mucor spp, trichophyton spp and candida albicans. the presence ...200415816270
investigation on the toxicity of fungi from rootstock snacks.chicken embryo bioassay was used to monitor the toxicity of extracts from rootstock snack samples during a 210-day storage period. results show that the relative toxicity values which were initially very low increased significantly as from 120th day (when 32% mortality was recorded) up till the last day when 73% was obtained. toxicity of extracts from axenic cultures of 12 fungal species isolated from the snack samples was also determined. the strains of aspergillus chevalieri, rhizopus nigrican ...19948145803
phytochemical and antimicrobial properties of constituents of "ogwu odenigbo", a popular nigerian herbal medicine for typhoid fever.the ethylacetate-insoluble fraction of the methanol extract of "ogwu odenigbo" a popular nigerian traditional herbal medicine for typhoid fever prepared from the stem bark of cleistropholis patens benth, (annonaceae), was separated into 13 semi-characterized constituents by preparatory tlc. the in vitro antimicrobial activities of the 13 fractions were quantitatively and/or qualitatively assessed by the agar well diffusion method using staphylococcus aureus, bacillus subtilis, escherichia coli, ...200111180528
etiological agents of otomycosis in nigeria.a total of 159 suspected cases of otomycosis comprising 101 adults and 58 children, 61 males and 98 females were investigated. of these, 36 cases were confirmed specifically of mycotic etiology on the basis of microscopic demonstration of fungal structures in epithelial debris/plugs and positive culture. another 31 cases positive for fungi by culture but negative for direct microscopy were considered of doubtful fungal etiology. the predominant etiological agents in the confirmed cases were aspe ...19892503721
effect of fungi fermentation on organoleptic properties, energy content and in-vitro multienzyme digestibility of cassava products (flour & gari).the present study sought to investigate the effect of fungi fermentation on the energy content, sensory quality and the digestibility (in vitro) of cassava products (flour and gari). the fungi fermented cassava products (gari and flour) were produced, by fermenting cassava mash with pure strains of some common saprophytes, namely, aspergillus flavus, aspergillus niger, rhizopus oryzae and saccharomyces spp (baker's yeast and palm wine yeast) for 72 hrs before processing into cassava flour and ga ...200314653508
preliminary investigation of in-vitro antimicrobial activity of two nigerian diospyros species (ebenaceae).the aqueous and alcoholic extracts from the leaves of diospyros bateri and d. monbuttensis were studied for their antimicrobial activities. the alcoholic extracts of the two diospyros spp. showed strong antibacterial activity against a wide range of gram-positive and gram-negative bacteria, while only the aqueous extract of d. bateri showed antibacterial activity against all the bacteria tested. the two fungi included in the study aspergillus niger and candida albicans were found to be resistant ...19882845757
production, acceptability and microbiological evaluation of weaning food formulations.weaning food formulations were produced from pearl millet cultivar (sosat c-88), cowpea and groundnut in the ratio of 70:20:10 and 5 per cent malt from six pearl millet cultivars (sosat c-88, zango, ex-borno, icmv is 94206, gwagwa, gb 8735) and one sorghum cultivar (icsv iii) were added to produce seven formulations plus the one without malt. microbiological quality of eight weaning food formulations was evaluated. twenty weaning mothers were used to determine the acceptability of gruels from th ...200616169859
microbial contamination of seven major weaning foods in nigeria.five million children aged less than five years die annually due to diarrhoea. the aim of the study was to identify some possible contributing factors for persistent diarrhoea. seven weaning foods, including a locally-made food, were evaluated by estimating the microbial load using the most probable number method and aflatoxin levels (afm1, afg1, afg2, and afb2) by immunoaffinity column extraction and high-performance liquid chromatography (hplc) with detection of fluorescence. the results showe ...201121957681
fungi associated with herbal drug plants during storage.samples of sundried herbal drug plant parts stored for sale were purchased from four herbal markets located in ibadan, nigeria. the plant parts were analysed for mycoflora associated with their storage. fungal colony counts ranged from 0.60 x 10(2) to 3.50 x 10(2) within the period of the survey. twenty eight fungal species were isolated with the species of aspergillus niger, a. flavus, fusarium moniliforme, trichoderma viride, penicillium expansum and mucor fragilis being the dominant ones. the ...19969208479
fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria.the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ...19969208478
influence of fungi associated with bananas on nutritional content during storage.botryodiplodia theobromae, rhizopus oryzae, aspergillus niger, a. flavus and fusarium equiseti were found to be associated with the ripening of bananas and also caused rot during storage. bananas stored in baskets with ash fire wood ripened 2-3 days earlier than bananas stored in fibre sacks and under constant light. the infected bananas showed a decrease in the quantity of total soluble sugars, protein, lipid, crude fibre, ash, ascorbic acid and mineral elements when compared with the control f ...19968711953
effects of sodium chloride and relative humidity on growth and sporulation of moulds isolated from cured fish.a total of 14 moulds were isolated from 84 samples of cured fish obtained from several processing centres and retail shops in lagos, ogun and oyo states, nigeria. aspergillus niger, a. flavus and a. chevalieri were the most prevalent. with the exception of basipetospora sp., slight inhibition of mycelial growth and/or sporulation was recorded when isolates were cultured in basal medium containing 5% sodium chloride. the extent of inhibition increased with increasing salt concentrations, and at 2 ...19948028651
fungi associated with stored unprocessed cowpea and groundnut varieties available in borno state, nigeria.five stored unprocessed cowpea (vigna spp) and four groundnut (arachis hypogeae) varieties available in borno state were examined for the mould flora. the degree of infestation of the grains ranged from 31% to 100% and 68% to 86% for surface sterilized cowpea and groundnut respectively. the mould flora commonly encountered were species of the genera aspergillus. penicillium species, scopulariopsis species and trichoderma species were also found. the flora of the cowpea was dominated by aspergill ...19921300349
role of opportunistic fungi in ocular infections in nigeria.the importance of opportunistic fungal pathogens in causing ocular infections is emphasized. a study was conducted over a period of 4 years (1974--1977) to investigate the role of opportunistic fungi in causing mycotic keratitis and to elucidate certain aspects of epidemiology of this disease in nigeria. fifty-nine cases of corneal ulcers of suspected mycotic etiology were investigated. fungal etiology was confirmed in 42 of these cases. the predominant etiological agent was fusarium solani in 1 ...1978745623
the fungal profile of otomycosis patients in ilorin, nigeria.the pathogenic fungi associated with ear infection among 142 patients with clinical signs of otomycosis are reported. 76(54%) were positive for at least one organism. the isolates included: aspergillus (63.2%), candida (35.5%), and mucor (0.7%). further analysis showed that candida species comprised c. albicans (18.4%), c. tropicalis (10.5%), and c. pseudotropicalis (6.6%), while a. fumigatus (39.5%), a. niger (23.7), and mucor (0.7%), were other significant pathogens. males and females were alm ...200111806011
pathogenic organisms in chronic suppurative otitis media in enugu, nigeria.pathogenic organisms in chronic suppurative otitis media from 62 patients were identified. pseudomonas aeruginosa was responsible in 46%, staphylococcus aureus in 29%. proteus mirabilis in 13%, streptococcus pyogenes in 6%, aspergillus niger in 5% and mucor sp. in 2%. the most prevalent organism in children was staphylococcus aureus. the role of fungi in chronic suppurative otitis media is discussed.19836422594
post-processing microflora and the shelf stability of gari marketed in port harcourt.gari was examined for its post-processing microbial content. aerobic mesophilic bacteria and fungi were isolated from all samples. the total viable bacterial counts ranged from 2.0 x 10(2) to 8.0 x 10(4) cfu/g. fungal counts ranged from 1.0 x 10(2) to 1.5 x 10(4) cfu/g. the total viable counts of fresh samples were much lower than those of market and packaged samples. bacillus, micrococcus and proteus spp. were the bacteria isolated, aspergillus niger, aspergillus flavus and penicillium spp. the ...19883170384
mycoflora and nutritional value of shelled melon seeds (citrulus vulgaris schrad.) in nigeria.thirteen fungi were isolated from mouldy shelled melon seeds. the fungi more frequently isolated included species of mucor (11.25%), rhizopus (13.75%), aspergillus (36.25%), macrophoma (2.50%), penicillium (8.75%), alternaria (5.00%), fusarium (8.75%), botrytis (6.25%), torula (3.75%) and geotrichum (3.75%). healthy, shelled melon seeds inoculated with fusarium solani for 7 or 14 days caused increases in the free fatty acid (f.f.a.) content of the healthy seeds from an initial value of 1.06% to ...19902170965
occurrence of keratinophilic fungi and dermatophytes on domestic birds in nigeria.feathers, nails and beaks of one hundred and twenty common birds in nigeria, chicken [50], ducks [20], turkeys [15] and pigeons [35], were examined using the soil plate technique for their mycoflora. 15 species of fungi were recovered and they belong to the genera chrysosporium, trichophyton, microsporum, aspergillus, fusarium, mucor rhizopus, penicillium and trichoderma. microsporum gypseum was the species most frequently isolated (35% of the samples). the most common genus was chrysosporium an ...200212000131
otomycosis in nigeria: treatment with mercurochrome.seventy-two mycologically proven cases of otomycosis (38 males, 34 females) aged 8-80 years were investigated. aspergillus niger was the commonest aetiological agent (43.1%) followed by candida spp. (22.2%), a. flavus (19.4%) and a. fumigatus (15.3%). three topical drugs, namely mercurochrome, clotrimazole and locacorten-vioform, were evaluated for otomycosis therapy in separate groups of 24 patients each. mercurochrome was found to be the most efficacious in terms of healing, relief from sympto ...200111766105
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