Publications

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novel genetic variants of anaplasma phagocytophilum, anaplasma bovis, anaplasma centrale, and a novel ehrlichia sp. in wild deer and ticks on two major islands in japan.wild deer are one of the important natural reservoir hosts of several species of ehrlichia and anaplasma that cause human ehrlichiosis or anaplasmosis in the united states and europe. the primary aim of the present study was to determine whether and what species of ehrlichia and anaplasma naturally infect deer in japan. blood samples obtained from wild deer on two major japanese islands, hokkaido and honshu, were tested for the presence of ehrlichia and anaplasma by pcr assays and sequencing of ...200616461655
molecular survey of anaplasma and ehrlichia infections of feral raccoons (procyon lotor) in hokkaido, japan.infection by anaplasma and ehrlichia in feral raccoons (procyon lotor) in hokkaido, japan, was examined by molecular methods. a polymerase chain reaction (pcr) screen for anaplasmataceae, based on 16s rrna, showed that 38 (5.4%) of 699 raccoons examined were positive. these 38 positive samples were examined for anaplasma phagocytophilum, anaplasma bovis, ehrlichia chaffeensis, and ehrlichia canis infection by species-specific nested pcr. nested pcr results indicated that 36 of the 38 samples wer ...201121395415
molecular survey for anaplasma bovis and anaplasma phagocytophilum infection in cattle in a pastureland where sika deer appear in hokkaido, japan.anaplasma bovis and anaplasma phagocytophilum infection were examined by species-specific nested polymerase chain reaction in cattle in a pastureland where sika deer appear in hokkaido, japan. of the 78 cattle examined, 12 (15%) and 1 (1%) tested positive for infection by a. bovis and a. phagocytophilum, respectively. one cattle tested positive for both. a. phagocytophilum infection rates were significantly lower in cattle than in sika deer (46%), but the infection rate by a. bovis was not signi ...200919168967
detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan.dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ...201019897306
detection of anaplasma phagocytophilum from wild boars and deer in japan.this study was conducted to determine the prevalence and geographic distribution of anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, in wild deer and boars in japan. we analyzed the blood samples of the study animals using pcr-targeting the 16s rdna followed by dna sequencing. wild deer infected with anaplasma spp., including anaplasma bovis and anaplasma centrale were detected in the region from hokkaido to kyushu. the infection rates of a. phagocytophilum, a. ...201121788712
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