Publications

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nosological study of borna disease virus infection in race horses.to investigate the prevalence of diseases in the borna disease virus (bdv) antibody positive race horses, we undertook seroepidemiological studies of bdv infection on 125 culled race horses in hokkaido, japan. the serological study disclosed the presence of antibodies only to bdv-p40 or -p24 in 19.2% (24/125) and 3.2% (4/125) of culled horses, respectively. antibodies to both bdv-p40 and -p24 were found in 24.0% (30/125) of these horses. of particular note was the finding that locomotorium disor ...200211750144
a serosurvey of borna disease virus infection in wild rats by a capture elisa.for a serological diagnostic test for borna disease (bd), we developed a capture elisa with specificity and sensitivity based on detection of antibodies against bd virus (bdv) p40 protein. using our capture elisa system, the antibody response of rats inoculated intracerebrally with bdv at 4 weeks after birth showed a sharp increase from 1 to 4 weeks postinoculation (p.i.) and a steady level after 5 weeks p.i. to investigate prevalence of bdv infection among wild rats, we examined sera of rattus ...199910081747
high prevalence of borna disease virus infection in healthy sheep in japan.previous seroepidemiological and molecular epidemiological studies of borna disease virus (bdv) showed considerably high rates of infection in horses, cattle, cats, and humans in hokkaido, japan. here, we further demonstrate high rates of specific antibodies to bdv and bdv rna in peripheral blood mononuclear cells (pbmcs) from healthy sheep bred on the same island. the bdv prevalences by immunoblotting and/or reverse transcriptase pcr were 0% (0 of 19) in newborns (<1 month old), 51.7% (15 of 29 ...19979144374
sero-epidemiological analysis of vertical transmission relative risk of borna disease virus infection in dairy herds.borna disease virus (bdv) is a virus that causes a neurological disease in domestic animals, including a variety of animal species in japan. few studies have examined the mode of transmission of this virus in cattle, and the exact mechanisms underlying the transmission of the virus need to be elucidated. this study aimed to examine the contribution of vertical transmission of the virus, which occurs when the virus is transmitted from the mother to offspring during gestation or birth. we used an ...201627498995
borna disease virus in raccoons (procyon lotor) in japan.we have examined the seroprevalence of bdv in wild raccoons (procyon lotor) in hokkaido, japan. serum samples from raccoons were examined using elisa and western blot assays to detect the presence of serum antibodies that react specifically to bdv antigens. among 549 investigated individuals, eleven (2.0%) showed a positive reaction to bdv antigens. brain tissue samples from five individuals were subjected to rt-pcr, which detected bdv sequences in three of them. sequence analysis revealed a hig ...200919721351
epidemiological survey of border disease virus among sheep from northern districts of japan.the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ...201121778667
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