Publications

first detection of microsporidia in dairy calves in north america.	fecal specimens were obtained from a total of 413 dairy calves from farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. after removal of fecal debris by sieving and density gradient centrifugation, specimens were examined by fluorescence microscopy, polymerase chain reaction (pcr), and dna sequencing analysis for the presence of microsporidia. microscopic examination revealed no spores. pcr using generic primers for microsporidia revealed 70 positive calves ...	2003	12739131
prevalence of enterocytozoon bieneusi in post-weaned dairy calves in the eastern united states.	fecal specimens were obtained from 3- to 8-month-old post-weaned dairy calves on farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. after removal of fecal debris by sieving and density gradient centrifugation, 59 of 452 calves (13%) from 11 farms in six states were found positive for enterocytozoon bieneusi by pcr and dna sequence analysis. based on gene sequence data this genotype of e. bieneusi found in post-weaned calves was 100% identical to that found ...	2004	15170566
molecular characterization of enterocytozoon bieneusi in cattle indicates that only some isolates have zoonotic potential.	in this study, 338 fecal samples were analyzed for enterocytozoon bieneusi from cattle farms in florida, maryland, michigan, new york, north carolina, pennsylvania, virginia, and portugal. the internal transcribed spacer region (392 bp) of the rrna gene of e. bieneusi was amplified using a nested pcr protocol. thirty-two e. bieneusi-pcr positive samples were sequenced. a high degree of genetic polymorphism, represented by five distinct genotypes (beb1-beb5), was found among the e. bieneusi isola ...	2004	14727187
enterocytozoon bieneusi genotypes in dairy cattle in the eastern united states.	fecal specimens were obtained from 12-24-month-old dairy heifers on farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. pcr positive specimens for enterocytozoon bieneusi were found in 131 of 571 heifers examined (23%) and on all the farms visited. the prevalence of e. bieneusi varied considerably across farms, with the lowest prevalence (4.7%) on md-2 and the highest prevalence (37.8%) on ny-2. all pcr positive specimens that amplified the its region as we ...	2005	16167161
enterocytozoon bieneusi in mature dairy cattle on farms in the eastern united states.	fecal specimens were obtained from mature milking cows on farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. polymerase chain reaction (pcr)-positive specimens for enterocytozoon bieneusi were found in 24 of 541 cows examined (4.4%) and on 12 of 14 farms. the prevalence of e. bieneusi varied considerably from farm to farm, with the lowest prevalence (2.3%) on fl-2 and the highest prevalence (12.5%) on vt-2. none of the cows exhibited signs of diarrhea. all ...	2007	17899197
host specificity and source of enterocytozoon bieneusi genotypes in a drinking source watershed.	to assess the host specificity of enterocytozoon bieneusi and to track the sources of e. bieneusi contamination, we genotyped e. bieneusi in wildlife and stormwater from the watershed of new york city's source water, using ribosomal internal transcribed spacer (its)-based pcr and sequence analyses. a total of 255 specimens from 23 species of wild mammals and 67 samples from stormwater were analyzed. seventy-four (29.0%) of the wildlife specimens and 39 (58.2%) of the stormwater samples from stre ...	2014	24141128